2 d oxy d glucose

Na₂SO₄ from Laboratoire MAT (Quebec City, QC, Canada), 1.0 M NaOH and HCl solutions as well as acetonitrile optima^® LC/MS were provided from Fisher Scientific (Montréal, QC, Canada). Trifluoroacetic acid (TFA) was obtained from J.T. Baker (Phillipsburg, NJ, USA) and KCl was purchased from ACP Inc (Montreal, QC, Canada). Fetal Bovine Serum (FBS) and trypsin (0.25% solution) were obtained from Invitrogen (Burlington, ON, Canada). Nylon filters were from Mandel Scientific (Guelph, ON, Canada). 2-déoxy-D-glucose (nonradioactive), CaCl₂, Hepes-Na and MgSO₄ were obtained from Sigma Aldrich (Oakville, ON, Canada), NaCl from VWR international (Montreal, QC, Canada). D-2-deoxy-[3H] glucose (radioactive) from Perkin Elmer (Woodbridge, ON, Canada). Finally, Pierce^® BCA Protein Assay Kit BCA, from Pierce Biotechnology (Rockford, IL, USA).

KCl was obtained from ACP Inc (Montreal, QC, Canada) and Na₂SO₄ from Laboratoire MAT (Québec city, QC, Canada). Formic acid, 1.0 M HCl, and 1.0 M NaOH solutions were from Fisher Scientific (Montreal, QC, Canada), trifluoroacetic acid was purchased from J.T. Baker (Phillipsburg, NJ, USA). NaCl, Acetonitrile optima^® liquid chromatography-mass spectrometry (LC/MS), and water grade were from VWR international (Montréal, QC, Canada). Concerning the glucose uptake experiments, the alpha-Minimal Essential Medium (α-MEM), Fetal Bovine Serum (FBS), and trypsin (0,25% solution) were obtained from Invitrogen (Burlington, ON, Canada). The 2-déoxy-D-glucose (non-radioactive), CaCl₂, Hepes-Na, and MgSO4 were purchased from Sigma Aldrich (Oakville, ON, Canada). D-2-deoxy-[³H] glucose was from Perkin Elmer (Woodbridge, ON, Canada) and Pierce^® BCA Protein Assay Kit BCA was from Pierce Biotechnology (Rockford, IL, USA). Insulin was from CHUL’s pharmacy (Québec, QC, Canada). Also, the L6 skeletal muscle cells line, derived from neonatal rat thigh skeletal muscle, were provided by Dr. A. Klip, Hospital for Sick Children (Toronto, ON, Canada).