Animal bed system

MRI was performed with a Bruker Biospec 9.4 Tesla instrument using Paravision 5.1 software (Bruker Biospin, Billerica, MA). A Bruker 72 mm ID volume coil was used for excitation and a custom 24 mm surface coil for signal reception (Doty Scientific Inc., Columbia, SC). Mice were anesthetized with isoflurane gas and respiration observed with a MRI-compatible physiological monitoring system (SA Instruments Inc., Stony Brook, NY). Animals were imaged in supine position on a Bruker animal bed system with circulating heated water to maintain body temperature. A 2D T2-weighted RARE sequence was used for imaging of the abdomen. The following imaging parameters were used: TR/TE = 2000/25ms, echo spacing = 12.5ms, ETL = 4, 2 averages, 29 contiguous axial slices with 1 mm thickness, FOV = 30×30 mm and matrix = 300×300 for an in-plane resolution of 100 µm. Prospective respiratory gating was used to minimize motion artifacts. Tumor volumes were quantitated using ImageJ software.

MRI was performed with a Bruker Biospec 9.4 Tesla instrument using Paravision 5.1 software (Bruker Biospin, Billerica, MA). A Bruker 72 mm ID volume coil was used for excitation and a custom 24 mm surface coil for signal reception (Doty Scientific Inc., Columbia, SC). Mice were anesthetized with isoflurane gas and respiration observed with a MRI-compatible physiological monitoring system (SA Instruments Inc., Stony Brook, NY). Animals were imaged in supine position on a Bruker animal bed system with circulating heated water to maintain body temperature. A 2D T2-weighted RARE sequence was used for imaging of the abdomen. The following imaging parameters were used: TR/TE = 2000/25 ms, echo spacing = 12.5 ms, ETL = 4, 2 averages, 29 contiguous axial slices with 1 mm thickness, FOV = 30 × 30 mm and matrix = 300 × 300 for an in-plane resolution of 100 μm. Prospective respiratory gating was used to minimize motion artifacts. Tumors volumes were quantitated using ImageJ software.