Cell proliferation analysis was performed by taking pictures as described (Chen et al., 2014 (link)). Briefly, H9c2 cells were seeded in DMEM supplemented with 10% FBS in 6-well plates at a density of 2 × 105 cells/well and grown overnight at 37°C in a humidified incubator with 5% CO2. The next day, maduramicin (0–1 μg/ml) was added. In some cases, cells were pretreated with okadaic acid for 1 h or infected with Ad-MKK1-R4F, Ad-dn-PP2A and Ad-GFP for 24 h. Then maduramicin (0.5 and 1 μg/ml) was added. After incubation for 48 h, images were taken with an Olympus inverted phase-contrast microscope equipped with the Quick Imaging system.
Quick imaging system
Manufactured by Olympus
Sourced in United States
The Quick Imaging system is a laboratory equipment designed for rapid image capture and analysis. It provides a fast and efficient way to acquire high-quality images of samples. The core function of the system is to enable quick and precise image acquisition for various scientific and research applications.
Automatically generated - may contain errors
9 protocols using quick imaging system
1
Cell Proliferation Analysis of H9c2 Cells
2
Evaluating FC101 Cytotoxicity in COS7 and HEK 293
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COS7 and HEK 293 cells were seeded at a density of 2×104 cells per well in 6-well plates. The next day, FC101 (0–5 µM) was added. After incubation for 4–6 days until the control cells were nearly 100% confluent, images were taken with an Olympus inverted phase-contrast microscope equipped with the Quick Imaging system. All cells were then trypsinized and enumerated using a Z1 Coulter Counter (Beckman Coulter, Fullerton, CA). Cells treated with vehicle alone (phosphate buffered saline, PBS) were used as a control.
3
Evaluation of FC101 and Pathway Modulators
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Cells were seeded at a density of 2 × 105 cells/well in a 6-well plate. The next day, cells were treated with FC101 (0–1 μM), following pre-incubation with/without SP600125 (1 μM) for 30 min. In some cases, after infection with Ad-PP2A, Ad-PP5 and Ad-GFP, respectively, cells were exposed to FC101 (0–1 μM) for 24 h. Additionally, cells were seeded at a density of 2 × 104 cells/well in a 6-well plate. The next day, the cells were exposed to FC101 (0–1 μM) for 4–6 days following pretreatment with or without NAC (5 mM) for 1 h. Finally, images were taken with an Olympus inverted phase-contrast microscope equipped with the Quick Imaging system.
4
Maduramicin Inhibits Cell Growth
5
Cell Morphology and Proliferation Assay
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Cell morphological analysis and cell proliferation assay were performed as described [24] (link). Briefly, cells were seeded in 6-well plates at a density of 1×104 cells/well and cultured in the growth medium overnight at 37°C in a humidified incubator with 5% CO2. The next day, maduramicin (0–1 µg/ml) was added. After incubation for 5 days, images were taken with an Olympus inverted phase-contrast microscope equipped with the Quick Imaging system. The cells were then trypsinized and enumerated using a Z1 Coulter Counter (Beckman Coulter, Fullerton, CA, USA). Cells treated with the vehicle (DMSO) alone served as a control.
6
Celastrol-Induced Cell Morphology Changes
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Cells were seeded at a density of 1 × 106 cells/well in a six-well plate. Next day, 0.5 μM celastrol was added. After 24 h, images were taken with an Olympus inverted phase-contrast microscope (Olympus Optical Co., Melville, NY, USA) (200×) equipped with the Quick Imaging system.
7
Evaluating CPX Effects on Rh30 and RD Cells
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Rh30 and RD cells were seeded in 6-well plates (2 × 105 cells/well). The next day, the cells were treated with CPX (0, 5 and 20 μM). After incubation for 72 h, images were taken with an Olympus inverted phase-contrast microscope (Olympus Optical Co., Melville, NY) (200×) equipped with the Quick Imaging system.
8
Brevinin-1RL1 Inhibits A549 and HCT116 Cell Growth
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A549 and HCT116 cells were seeded (1 × 105 cells/well) in complete culture medium. The next day, the cells were treated with Brevinin-1RL1 (0, 6 and 12 μM) for 24 h, images were taken with an Olympus inverted phasecontrast microscope (Olympus Optical Co., Melville, NY, USA) (200×) equipped with the Quick Imaging system.
9
Neuroprotective Effects of Rapamycin
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PC12 cells, SH-SY5Y cells and primary neurons were seeded in a PDL-uncoated or -coated 6-well plate (5 × 105 cells/well) or 96-well plate (1 × 104 cells/well). The next day, the cells were exposed to Cd (10 and/or 20 μM) for 24 h following pre-incubation with/without rapamycin (200 ng/ml) for 48 h, with 5 replicates of each treatment. Subsequently, the images for morphological analysis were taken under an Olympus inverted phase-contrast microscope (Olympus Optical Co., Melville, NY, USA) (200 ×) equipped with the Quick Imaging system. Caspase-3/7 activity was determined using Caspase-Glo® 3/7 Assay Kit (Promega, Madison, WI, USA), following the instructions of the supplier.
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