Kaempferol (K) and luteolin (L) were purchased from Indofine (Hillsborough, New Jersey, USA). Tamarixetin (T), (-)-epicatechin (EC), (-)-gallocatechin (GC), (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG) were purchased from Extrasynthese (Genay, France). Quercetin (Q), isorhamnetin (I), Dimethyl sulfoxide (DMSO), Mc Coy's 5A medium, poly-DL-alanine and green tea catechins (GTC) mix (containing (+)-catechin (C), (-)-catechin gallate (CG), EC, ECG, EGCG, GC and (-)-gallocatechin gallate (GCG), 100 µg/mL of each component delivered in acetonitrile: water (8:2) with 5% 1 M HCl) were purchased from Sigma-Aldrich (Schnelldorf, Germany). Penicillin, streptomycin, Dulbecco's phosphate buffered saline (DPBS) and GlutaMax were purchased from Gibco TM (Life Technologies, Paisley, United Kingdom). Foetal bovine serum (FBS) was purchased from Lonza TM (VWR, Verviers, Belgium). Ascorbic acid was purchased from Prolabo TM (VWR, Leuven, Belgium). Acetonitrile (ACN) and formic acid (UPLC-MS grade) were purchased from BioSolve BV (Valkenswaard, The Netherlands). High-purity water was produced with a Synergy UV water purification system from Merck Millipore (Amsterdam, The Netherlands).
Poly dl alanine
Manufactured by Merck Group
Sourced in Germany, United States, United Kingdom
Poly-DL-alanine is a synthetic polymer composed of the amino acid DL-alanine. It is used as a laboratory reagent and research tool for various applications, such as in the study of protein structure and function.
Automatically generated - may contain errors
Lab products found in correlation
Trifluoroacetic acid tfa, by Merck Group (3 mentions)
Acetaminophen, by Merck Group (2 mentions)
Taurine, by Merck Group (2 mentions)
Milli q water ddh2o, by Merck Group (2 mentions)
Conductive indium tin oxide coated glass slides, by Bruker (2 mentions)
Superfrost plus, by Thermo Fisher Scientific (2 mentions)
1 5 diaminonaphtalene 1 5 dan 97.0 maldi matrix, by Merck Group (2 mentions)
Acetonitrile acn, by Avantor (2 mentions)
L alanine, by Merck Group (2 mentions)
Human liver microsomes hlm, by Sekisui (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Ascorbic acid, by Avantor (1 mentions)
Epicatechin, by Extrasynthese (1 mentions)
Gallocatechin gallate, by Merck Group (1 mentions)
Catechin gallate, by Merck Group (1 mentions)
Tamarixetin, by Extrasynthese (1 mentions)
Epigallocatechin gallate (egcg), by Extrasynthese (1 mentions)
Gallocatechin, by Extrasynthese (1 mentions)
Epicatechin gallate, by Extrasynthese (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
9 protocols using poly dl alanine
1
Bioactive Compound Acquisition and Preparation
2
Metabolite Profiling of M. balsamina Leaves
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HPLC-grade acetonitrile and methanol were purchased from Romil (Microsep, Johannesburg, Gauteng, South Africa). Formic acid was obtained from Merck (Darmstadt, Hessen, Germany) and deionised water was obtained using a Milli-Q water purification system (Millipore, Milford, MA, USA). Poly-DL-alanine used for TWCCSN2 calibration was purchased from Sigma-Aldrich (St. Louis, MO, USA). M. balsamina leaves were collected from Letsitele (23°57′51″ S 30°22′39″ E), Goedplaas (23°31′34.0″ S 30°03′20.2″ E) and Mankweng (23°53′47.7″ S 29°43′44.1″ E) in the Limpopo province of South Africa and subsequently identified at the Larry Leach Herbarium at the University of Limpopo.
3
Analysis of Benzalkonium Chlorides and Analogs
4
Lipid Standard Validation Protocol
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Lipid standards (Table S1) used in the external validation were purchased from Avanti polar lipid Co., Ltd (Sweden). LC-MS grade acetonitrile (ACN), isopropyl alcohol (IPA) and methanol (MeOH) were purchased from Thermo Fisher Scientific Ltd. Co (Boston, USA).
Ultrapure water was obtained using an 18 Ω m Milli-Q system (Millipore Corporation, Billerica, MA). Poly-DL-alanine was purchased from Sigma-Aldrich (U.K.). Male zebrafish were purchased from Model Animal Research Center of Nanjing University (Nanjing, China). All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Shenzhen Research Institute of The Hong Kong Polytechnic University and approved by the Animal Ethics Committee of Shenzhen Research Institute of The Hong Kong Polytechnic University.
Ultrapure water was obtained using an 18 Ω m Milli-Q system (Millipore Corporation, Billerica, MA). Poly-DL-alanine was purchased from Sigma-Aldrich (U.K.). Male zebrafish were purchased from Model Animal Research Center of Nanjing University (Nanjing, China). All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Shenzhen Research Institute of The Hong Kong Polytechnic University and approved by the Animal Ethics Committee of Shenzhen Research Institute of The Hong Kong Polytechnic University.
5
MALDI-MRMS Metabolite Profiling
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All reagents were of HPLC grade. Milli-Q water (ddH2O; Millipore) was prepared in-house.
Conductive indium tin oxide (ITO) coated glass slides were purchased from Bruker Daltonics (Bremen, Germany). Adhesive slides SuperFrost Plus TM were obtained from Thermo Fisher Scientific (Waltham, Massachusetts, USA) for histological analysis. Trifluoroacetic acid (TFA) and 1,5-diaminonaphtalene (1,5-DAN, ≥ 97.0%) MALDI matrix were purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile (ACN) was obtained from VWR (Darmstadt, Germany). For external calibration of the Bruker solarix magnetic resonance mass spectrometer (MRMS), a mixture of poly-DL-alanine (10 mg/mL), L-alanine ≥ 99.5% (5 mg/mL) and taurine ≥ 99% (5 mg/mL; all from Sigma Aldrich) in water was used.
Conductive indium tin oxide (ITO) coated glass slides were purchased from Bruker Daltonics (Bremen, Germany). Adhesive slides SuperFrost Plus TM were obtained from Thermo Fisher Scientific (Waltham, Massachusetts, USA) for histological analysis. Trifluoroacetic acid (TFA) and 1,5-diaminonaphtalene (1,5-DAN, ≥ 97.0%) MALDI matrix were purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile (ACN) was obtained from VWR (Darmstadt, Germany). For external calibration of the Bruker solarix magnetic resonance mass spectrometer (MRMS), a mixture of poly-DL-alanine (10 mg/mL), L-alanine ≥ 99.5% (5 mg/mL) and taurine ≥ 99% (5 mg/mL; all from Sigma Aldrich) in water was used.
6
MALDI-MRMS Imaging Mass Spectrometry
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All reagents were of HPLC grade. Milli-Q water (ddH2O; Millipore) was prepared in-house. Conductive indium tin oxide (ITO)-coated glass slides were purchased from Bruker Daltonics (Bremen, Germany). Adhesive slides SuperFrost PlusTM were obtained from Thermo Fisher Scientific (Waltham, Massachusetts, USA) for histological analysis. Trifluoroacetic acid (TFA) and 1,5-diaminonaphtalene (1,5-DAN, ≥97.0%) MALDI matrix were purchased from Sigma-Aldrich (St. Louis, MO). 4-phenyl-α-cyanocinnamic acid amide (PhCCAA) matrix was obtained from SiChem (Bremen, Germany). Acetonitrile (ACN) was obtained from VWR (Darmstadt, Germany). For external calibration of the Bruker solarix magnetic resonance mass spectrometer (MRMS), a mixture of poly-DL-alanine (10 mg/mL), L- alanine ≥ 99.5% (5 mg/mL), and taurine ≥ 99% (5 mg/mL; all from Sigma-Aldrich) was used in water.
7
Proteomic Sample Preparation Protocol
8
Phospholipid and Metabolite Standards for Lipidomics
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Phosphatidylcholine (PC) and
phosphatidylethanolamine (PE) standards (Supporting Information Document 1, Table S1 ) and SPLASH Lipidomix
were acquired from Avanti Polar Lipids. Acetaminophen, caffeine, carnosine, S-(5′adenosyl)-l -methionine (SAM), and poly-dl -alanine were purchased from Sigma-Aldrich. Adenosine 5′-monophosphate
(AMP) was purchased from Cayman Chemical.l -Histidine, sucrose,
ammonium formate, formic acid (FA), HPLC grade acetonitrile (ACN),
methanol (MeOH), 2-propanol (BuOH), and water were purchased from
ThermoFisher Scientific. MassPREP enolase digest and alcohol dehydrogenase
digest were purchased from Waters. NIST SRM 1950 was acquired from
the National Institute of Standards and Technology (NIST).
phosphatidylethanolamine (PE) standards (
were acquired from Avanti Polar Lipids. Acetaminophen, caffeine, carnosine, S-(5′adenosyl)-
(AMP) was purchased from Cayman Chemical.
ammonium formate, formic acid (FA), HPLC grade acetonitrile (ACN),
methanol (MeOH), 2-propanol (BuOH), and water were purchased from
ThermoFisher Scientific. MassPREP enolase digest and alcohol dehydrogenase
digest were purchased from Waters. NIST SRM 1950 was acquired from
the National Institute of Standards and Technology (NIST).
9
Poly-DL-Alanine Characterization Protocol
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All materials were obtained from Sigma-Aldrich
(Germany) unless stated otherwise. Acetonitrile (ACN) was purchased
from Merck KGaA, (Darmstadt, Germany). Water was obtained using an
18 Ω m Milli-Q (EMD Millipore Corporation, Billerica, MA). All
chemicals and solvents were of analytical grade or higher purity.
Poly-dl -alanine (product number P9003) was purchased from
Sigma-Aldrich (U.K.). Platelets (PLT), red blood cells (RBC), and
plasma were obtained from the Blood Bank, Landspitali-University Hospital,
Reykjavik, Iceland). Urine was obtained from a male volunteer. This
study was approved by the National Bioethics Committee of Iceland
and the Icelandic Data Protection Authority.
(Germany) unless stated otherwise. Acetonitrile (ACN) was purchased
from Merck KGaA, (Darmstadt, Germany). Water was obtained using an
18 Ω m Milli-Q (EMD Millipore Corporation, Billerica, MA). All
chemicals and solvents were of analytical grade or higher purity.
Poly-
Sigma-Aldrich (U.K.). Platelets (PLT), red blood cells (RBC), and
plasma were obtained from the Blood Bank, Landspitali-University Hospital,
Reykjavik, Iceland). Urine was obtained from a male volunteer. This
study was approved by the National Bioethics Committee of Iceland
and the Icelandic Data Protection Authority.
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