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17β estradiol e2 e2758

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17β-estradiol (E2) (E2758) is a chemical compound that is commonly used as a reference standard in laboratory research and analysis. It is the primary and most potent natural estrogen in humans and other mammals. The compound has a molecular formula of C18H24O2 and a molar mass of 272.38 g/mol.

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Lab products found in correlation

Tamoxifen citrate t9272, by Merck Group (2 mentions) Col1a1 nb600 450, by Novus Biologicals (2 mentions) Dual luciferase assay system, by Promega (1 mentions) Sc 30080, by Santa Cruz Biotechnology (1 mentions) Pierce mitochondria isolation kit, by Thermo Fisher Scientific (1 mentions) Coomassie protein assay kit, by Thermo Fisher Scientific (1 mentions) 3 nitrotyrosine elisa kit, by Abcam (1 mentions) Ab3576, by Abcam (1 mentions) Ab9051, by Abcam (1 mentions) Ab17339, by Abcam (1 mentions) Ab6002, by Abcam (1 mentions) Ab24684, by Abcam (1 mentions) Ab9053, by Abcam (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) 5 bromouridine, by Merck Group (1 mentions) Anti brdu, by BD (1 mentions) Alamarblue assay, by Thermo Fisher Scientific (1 mentions) Lowry assay, by Bio-Rad (1 mentions)

Spelling variants of this product

17β-estradiol (455 citations) 17β-estradiol (E2) (283 citations) Estradiol (242 citations) Estradiol (E2) (50 citations) E2758 (42 citations) 17β-estradiol (17β-E2, (4 citations)

5 protocols using 17β estradiol e2 e2758

1

Antibody-based Protein Quantification in Cellular Studies

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Antibodies for β-actin (sc-47778), COX2 (sc-19999), MMP1 (sc-21731), NRF1 (sc-101102), and SOD2 (sc-30080) were obtained from Santa Cruz Biotechnology. Antibodies for acetyl-histone H4 K5, K8, K12, and K16 (H4K5,8,12,16ac, #PA5-40084) were obtained from Invitrogen. Antibodies for anti-histone H3 acetyl K9, K14, K18, K23, K27 (H3K9, 14, 18, 23, 27ac, ab47915), ERα (ab3575), ERβ (ab3576), H4K20me1 (ab9051), H4K20me3 (ab9053), H4R3me1 (ab17339), H3K9me2 (ab1220), H3K9me3 (ab8898), H3K27me2 (ab24684) and H3K27me3 (ab6002), H2AX (ab20669), and γH2AX (ab2893) were obtained from Abcam. The antibody for 8-oxo-dG (4354-MC-050) was obtained from Novus Biologicals. The 3-nitrotyrosine (3-NT) was measured using 3-Nitrotyrosine ELISA Kit (ab116691 from Abcam) per manufacturers’ instructions. The mitochondrial fraction was isolated using a Pierce Mitochondria Isolation Kit (Pierce Biotechnology) according to manufacturers’ instructions. The protein concentration was measured using the Coomassie Protein Assay Kit (Pierce Biotechnology) per manufacturers’ instructions. Luciferase activity assay was carried out using the Dual-Luciferase™ Assay System (Promega) and the transfection efficiency was normalized using a cotransfected renilla plasmid (23 (link)). 17β-estradiol (E2, #E2758) and TNFα (#T0157) were obtained from Sigma.
Xiang D., Zhao M., Cai X., Wang Y., Zhang L., Yao H., Liu M., Yang H., Xu M., Li H., Peng H., Wang M., Liang X., Li L, & Yao P. (2020). Betulinic Acid Inhibits Endometriosis Through Suppression of Estrogen Receptor β Signaling Pathway. Frontiers in Endocrinology, 11, 604648.
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2

Immunohistochemical Analysis of Breast Cancer

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17β-estradiol (E2) (E2758) and tamoxifen citrate (T9272) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Biotinylated goat anti-rabbit (BA-100), Avidin-biotin-complex (ABC) (PK-4000), ImmPACT DAB (SK-4105) were purchased from Vector Labs (Burlingame, CA, USA). Antibodies against the following proteins were purchased from these vendors: STAT5A (sc-1081), ERα (sc-542), Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); progesterone receptor (PR, A0098), Dako (Carpinteria, CA, USA); Ki-67 (ab15580), FN1 (ab23750), POSTN (ab14041), Abcam (Cambridge, MA, USA); c-JUN (cs 9165), PAK1 (cs 2602), pAKT S473 (cs 3787; cs9271), AKT (cs9272), pERK1/2 (cs 9101), ERK1/2 (cs9102), Cell Signaling Technology (Danvers, MA, USA), COL1A1 (NB600–450), Novus Biologicals, Centennial, CO, USA).
Jallow F., O’Leary K.A., Rugowski D.E., Guerrero J.F., Ponik S.M, & Schuler L.A. (2019). Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer. Oncogene, 38(43), 6913-6925.
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3

Immunohistochemical Analysis of Breast Cancer

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17β-estradiol (E2) (E2758) and tamoxifen citrate (T9272) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Biotinylated goat anti-rabbit (BA-100), Avidin-biotin-complex (ABC) (PK-4000), ImmPACT DAB (SK-4105) were purchased from Vector Labs (Burlingame, CA, USA). Antibodies against the following proteins were purchased from these vendors: STAT5A (sc-1081), ERα (sc-542), Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA); progesterone receptor (PR, A0098), Dako (Carpinteria, CA, USA); Ki-67 (ab15580), FN1 (ab23750), POSTN (ab14041), Abcam (Cambridge, MA, USA); c-JUN (cs 9165), PAK1 (cs 2602), pAKT S473 (cs 3787; cs9271), AKT (cs9272), pERK1/2 (cs 9101), ERK1/2 (cs9102), Cell Signaling Technology (Danvers, MA, USA), COL1A1 (NB600–450), Novus Biologicals, Centennial, CO, USA).
Jallow F., O’Leary K.A., Rugowski D.E., Guerrero J.F., Ponik S.M, & Schuler L.A. (2019). Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer. Oncogene, 38(43), 6913-6925.
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4

Estrogen Receptor Signaling Pathway Assay

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EHT 1864 was purchased from APExBIO (B5487). 17-β-estradiol (E2) (E2758), 4-hydroxytamofen (OHT) (H7904), Fulvestrant (I4409), and 5-bromouridine (850187) were purchased from Sigma. MCF-7 cells were maintained in Dr. Marc Lippman’s laboratory. Other cell lines were from ATCC. Cell lines were authenticated with short tandem repeat profiling and were regularly tested for mycoplasma in house. MCF-7 cells were cultured in IMEM (Invitrogen) with 5% fetal calf serum (FBS) (Hyclone). Other cells were maintained in DMEM (Invitrogen) with 10% FBS. For estrogen deprivation, the cells were shifted to IMEM containing 5% charcoal-treated FBS (cFBS) (Hyclone) for three days. The antibodies against ER (sc-543 and sc-8002) and SRC-3 (sc-7216) were from Santa Cruz Biotechnology. The antibodies against RAC1 were from ThermoFisher Scientific (PA1091) and Millipore (clone 23A8, 05-389). RAC3 antibody was from Abcam (ab129062). AR antibody was from Millipore (06-680). Anti-BrdU was from BD Biosciences (555627).
Sun J., Gaidosh G., Xu Y., Mookhtiar A., Man N., Cingaram P.R., Blumenthal E., Shiekhattar R., Goka E.T., Nimer S.D, & Lippman M.E. (2021). RAC1 plays an essential role in estrogen receptor alpha function in breast cancer cells. Oncogene, 40(40), 5950-5962.
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5

Antibody Validation for APLNR and AMPK

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Antibodies raised against apelin receptor (APLNR) (sc-517300, mouse) were from Santa Cruz Biotechnology (Dallas, Texas, USA); antibodies against total AMPKα (2532, rabbit), phospho-AMPKα (Thr 172 ) (2535, rabbit) were obtained from Cell Signaling Technology (Beverly, MA, USA); antibody raised against fluorescein isothiocyanate (FITC) conjugated-α-Tubulin (F2168, rabbit) was purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies (111-035-003, rabbit) and (115-035-003, mouse) were purchased from Cedarlane (Burlington, Canada). 17β-estradiol (E2; E2758) was purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Apelin-13 (13523) was obtained from Cayman Chemical, (Michigan, USA). Lowry assay was obtained from Bio-Rad (Hercules, CA). Western Blot Chemiluminescence Reagent Plus system was obtained from (NEN Life Science Products). AlamarBlue assay was purchased from Invitrogen (Indianapolis, USA). The Lysotracker green (L7526) was purchased from Thermofisher (San Diego, California, USA).
Bouchelaghem R., Mader S., Gaboury L., Messarah M., Boumendjel M, & Boumendjel A. (2022). Estrogens desensitize MCF-7 breast cancer cells to apelin-induced autophagy and enhanced growth under estrogen starvation: a possible implication in endocrine resistance. Cellular and molecular biology (Noisy-le-Grand, France), 68(9).
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