Pl aquagel oh column

AAPS and the AAPS-iron(III) complex were each dispersed in ultrapure water to make the solutions (2% g/ml). The particle size distributions of the samples were determined by a laser particle size analyzer (Mastersizer3000, Malvern Instruments Ltd, United Kingdom) with a shading rate of 10-20% and scattering intensity of 35. The relative molecular weights of AAPS and AAPS-iron(III) complex were estimated on an Agilent 1100 HPLC system equipped with a refractive index detector (RID) and a PL aquagel-OH Column (79911GF-083, Agilent). The sample was filtered on a 0.45-μm pore membrane before injection (20 μL) and eluted with Na₂SO₄ solution (0.1 M) in PBS buffer (0.01 M, pH 6.8) at a flow rate of 0.7 mL/min. The column temperature was maintained at 40°C.

The thermal stability of O-acetylated glucomannan was determined by differential scanning calorimetry (DSC) and size-exclusion chromatography–high-performance liquid chromatography (SEC–HPLC). Samples solutions were prepared at the pH indicated unless specified otherwise (Figure 2). Two sets of identical samples of each polysaccharide were prepared: One set for DSC analysis and the other set for HPLC analysis. The storage temperature of polysaccharides for HPLC analysis was 65 °C. A MicroCal VP-DSC system (Malvern Instruments, Malvern, UK) was used at 25–90 °C with a scan rate of 1 °C/min. Analyses of thermograms were done with Origin™ software (OriginLab, Northampton, MA, USA). All experiments were carried out at a polysaccharide concentration of 2 mg/mL for DSC and HPLC analyses. For SEC-HPLC, 0.1 M NaCl was used as the eluent at a flow rate of 0.5 mL/min and the injection volume was 20 µL; a PL aquagel-OH column (Agilent Technologies, Santa Clara, CA, USA) was employed at 30 °C.

¹H NMR spectra were recorded on a spectrometer operating at 400 MHz (Bruker AMX-400). The polydispersity of PBLG-Br were determined by gel permeation chromatography (GPC) (Agilent 1260). The measurements were performed using DMF as the eluent at a flow rate of 1 ml/min at 40°C and a series of narrow PMMA standards for the calibration of the columns. The GPC measurement of PLG-b-PMPC diblock copolymer was conducted on a water GPC system (Agilent PL aquagel-OH column, EcoSEC (HLC-8320GPC with distilled water as eluent (25°C, flow rate: 1 ml/min, and polyethylene glycol as standards). Transmission electron microscopy (TEM) measurements were performed on a Hitachi H-600 transmission electron microscope with an accelerating voltage of 100 KV. A drop of the micelle solution (1 mg/ml) was deposited onto a 230 mesh copper grid and treated with 2% phosphotungstic acid at pH 6.5 negative stain solution, then allowed to dry at room temperature before measurements. Inductively coupled plasma mass spectrometry (ICP-MS, VG PQExCell, Thermo Jarrell Ash, USA) was used for determined the quantity of CDDP.