1260 infinity gpc instrument

Molecular weights
and dispersities were determined for the various homopolymers and
diblock copolymers using an Agilent 1260 Infinity GPC instrument.
The setup comprised a pump, a degasser, three columns in series (PL-Aquagel
Mixed-H, OH-30, and OH-40), and a refractive index detector. The column
and detector temperature was set at 30 °C, and the flow rate
was 1.0 mL min^–1. Calibration was achieved using
nine near-monodisperse poly(ethylene oxide) standards (2.1–969
kDa), and data were analyzed using Agilent Technologies GPC/SEC software.
For PMPC_x and its copolymers, the eluent
was an aqueous solution containing 0.20 M NaNO₃ and 0.05
M Trizma buffer at pH 7.0. For PATAC_x and
its copolymers, the eluent was an aqueous solution containing 0.50
M CH₃COOH and 0.30 M NaH₂PO₄ at pH
2.0. For PAMPS_x and its copolymers, the
eluent was a 70% v/v aqueous solution containing 0.20 M NaNO₃ and 0.01 M NaH₂PO₄ at pH 7.0, with 30% v/v
methanol.

Molecular weights
and dispersities were determined for the various homopolymers and
diblock copolymers using an Agilent 1260 Infinity GPC instrument.
This setup comprised a pump, a degasser, two PL-gel 5 μm Mixed-C
columns in series, and a refractive index detector. HPLC-grade DMF
containing 10 mM LiBr was used as the eluent, the column and detector
temperature was set to 60 °C, and the flow rate was 1.0 mL min^–1. Calibration was achieved using ten near-monodisperse
poly(methyl methacrylate) standards (370–2,520,000 g mol^–1), and data were analyzed using Agilent Technologies
GPC/SEC software.