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Mouse antibody against asah1 612302

Manufactured by BD
Sourced in United States

The mouse antibody against ASAH1 (612302) is a laboratory reagent used in research applications. It is designed to detect and study the ASAH1 (Acid Sphingomyelinase-Like Phosphodiesterase 3A) protein, which is involved in various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and other immunoassays to investigate the expression and localization of the ASAH1 protein in biological samples. The core function of this product is to provide researchers with a tool for the investigation of ASAH1 in their research studies.

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2 protocols using mouse antibody against asah1 612302

1

Antibody-based targeting of Sph-1P in cancer cells

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Mouse antibody against ASAH1 (612302) was purchased from BD Biosciences (San Jose, CA, USA). Anti-actin, carmofur, temozolomide (TMZ), and N-oleoylethanolamine (OE), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HRP-conjugated goat anti-mouse IgG was supplied by R&D Systems, Inc. (Minneapolis, MN, USA). SDS-PAGE and western blot materials were obtained from Life Technologies, Inc. (Grand Island, NY, USA). Murine anti-Sph-1P monoclonal antibody, (LT1002) and humanized anti-Sph-1P monoclonal antibody (LT1009) were obtained from Lpath, Inc. (16 (link),17 (link)).
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2

Antibody-based Assay for ASAH1

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Mouse antibody against ASAH1 (612302) was purchased from BD Biosciences (San Jose, CA). Anti-actin, carmofur, temozolomide (TMZ), and N-oleoylethanolamine (OE), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Aldrich (St. Louis, MO). DMSO, used to dissolve carmofur, temozolomide, and OE, was purchased from Sigma Aldrich (St. Louis, MO). HRP-conjugated goat anti-mouse IgG was supplied by R&D Systems, Inc. (Minneapolis, MN). SDS-PAGE and Western blotting materials and the Annexin V kit were obtained from Life Technologies, Inc. (Grand Island, NY).
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