Anti p21

Manufactured by Bioss Antibodies

Sourced in China

Anti-p21 is a primary antibody that specifically binds to the p21 protein. p21 is a cyclin-dependent kinase inhibitor that plays a role in cell cycle regulation. This antibody can be used for various research applications, such as Western blotting, immunohistochemistry, and flow cytometry, to detect and analyze the p21 protein.

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Lab products found in correlation

Brown dab precipitate, by Maixin Group (1 mentions) Anti cdk4, by Bioss Antibodies (1 mentions) Anti cdk6, by Bioss Antibodies (1 mentions) Anti ki67, by Maixin Group (1 mentions) Anti phb2, by Abcam (1 mentions) Anti cdk2, by Abcam (1 mentions) Anti lc3, by Abcam (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti mouse ck 14, by BioLegend (1 mentions) Anti s nitrosocysteine, by Abcam (1 mentions) Anti cd44, by Bioss Antibodies (1 mentions) Anti human ck 14, by Thermo Fisher Scientific (1 mentions) Anti human ck 18, by Thermo Fisher Scientific (1 mentions) Anti dynll1, by Abcam (1 mentions) Anti erbb2, by Thermo Fisher Scientific (1 mentions) Anti gm130, by Cell Signaling Technology (1 mentions) Anti p27, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti v5, by Thermo Fisher Scientific (1 mentions) Anti β tubulin, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti p21

Immunohistochemical Analysis of Senescence and Mitophagy

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Immunohistochemistry (IHC) staining was performed to detect the expression of senescence and mitophagy proteins. After antigens repaired, all sections were incubated with the primary antibody overnight at 4°C after blockage of endogenous peroxidases. The primary antibodies were used as follows: anti-Ki67 (Maixin), anti-wtp53 (1:300, Bioss), anti-p21 (1:300, Bioss), anti-CDK2 (1:50, Abcam), anti-CDK4 (1:100, Bioss), anti-CDK6 (1:400, Bioss), anti-PHB2 (1:200, Abcam) and anti-LC3 (1:100, Abcam). Next, the sections were incubated with secondary antibody (Maixin, China) at 37°C for 30 minutes. Finally, slides were evaluated using the brown DAB precipitate (Maixin, China) and were performed with hematoxylin.
All sections were observed under the microscope (Olympus BX61, Japan) to randomly select three regions at 200× magnification. Image-Pro Plus software was used for MOD quantitative analyses (MOD = integral optical density/measurement area).

Lu Y., Li L., Chen H., Jing X., Wang M., Ge L., Yang J., Zhang M, & Tang X. (2021). Peroxiredoxin1 Knockdown Inhibits Oral Carcinogenesis via Inducing Cell Senescence Dependent on Mitophagy. OncoTargets and therapy, 14, 239-251.

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Comprehensive Antibody Protocol for Cell Analysis

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The following antibodies were used. Anti-β-Galactosidase (Bioss, bs-4960R), anti-alkaline phosphatase (Novus Biologicals, NBP1-32948); anti-heterochromatin protein 1-γ (HP1-γ, phospho Ser 93, Bioss, bs-3221R); anti-p21 (Bioss, bs-10129R); anti-p27 (phospho Thr 187, Abcam, ab75908); anti-NOS-1, 2 (Thermo Fisher Scientific, PA1-033 and -036); anti-NOS-3 (Sigma-Aldrich, SAB-4300435); anti-ErbB2 (Thermo Fisher Scientific, PA5-16395); anti-pSMAD3 (Ser423/Ser425, Novous Biological, NBP1-77836); anti-CD24 (Novus Biologicals, NBP1-4639055); anti-CD44 (Bioss, bs-2507R); anti-S-Nitroso-Cysteine (Abcam, ab50185 or Alpha Diagnostics, NISC11-A); anti-Integrin α6 (BD Biosciences, 555734); anti-GM130 (Cell Signaling, 12480 S); anti-human CK 14 (ThermoFisher, MA511599); anti-human CK 18 (ThermoFisher, PA514263); anti-mouse CK 14 (BioLegend, 905301); anti-human CK 8/18 (DSHB, Troma-I); anti-Cleaved Caspase3 (Cell Signaling, #9664); anti-β-Actin (Sigma, A1978); anti-DYNLL1 (Abcam, ab51603); and anti-ADMA (EMD Millipore, 09-814).

Ren G., Zheng X., Bommarito M., Metzger S., Walia Y., Letson J., Schroering A., Kalinoski A., Weaver D., Figy C., Yeung K, & Furuta S. (2019). Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFβ. Scientific Reports, 9, 6688.

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Apoptosis Signaling Pathway Analysis

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Cisplatin (HY-17394), Z-VAD-FMK (HY-16658), doxorubicin (HY-15142), and staurosporine (62996-74-1) were purchased from MCE (Middlesex, NJ, USA). ML216 was purchased from MCE (HY-12342) and Selleck (S0469, Houston, TX, USA). Hydroxyurea (V900323) and etoposide (E1383) were purchased from Sigma (St. Louis, MO, USA). The antibodies used included anti-DBC1 (Bethyl Labotatories, Montgomery, TX, USA, A300-434A), anti-V5 (Invitrogen, Waltham, MA, USA, R960-25), anti-Flag (Sigma, F7425), anti-BLM (Bethyl Labotatories, A300-110A), anti-GAPDH (Millipore, St. Louis, MO, USA, MAB374), anti-p21 (BD Biosciences, San Jose, CA, USA, 556430), anti-β Tubulin (Santa Cruz, Dallas, TX, USA, SC-166729), anti-p53 (Santa Cruz, SC-126), anti-Bcl-2 (Santa Cruz, SC-7382), anti-p16 (Santa Cruz, SC-56330), anti-phospho-H2A.X (Millipore, 07-164-25UG), anti-β-actin (Absin, Shanghai, China, ABS830031), anti-PARP1 (CST, Danvers, MA, USA, 9542S), anti-cleaved PARP1 (CST, 5625S), anti-BLM (Bioss, Beijing, China, bs-12872R) and anti-p21 (Bioss, bs-0741R). Secondary antibodies were horseradish peroxidase-coupled sheep anti-mouse IgG (GE Healthcare, Chicago, IL, USA, NA931 or ZSGB-BIO, Beijing, China, ZB-2301), donkey anti-rabbit IgG (GE Healthcare, NA934), and horseradish peroxidase-coupled sheep anti-mouse IgG (ZSGB-BIO, ZB-2305).

Cui F., Han X., Zhang X., Wang S., Liang N., Tan Q., Sha W, & Li J. (2022). ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1–BLM Interaction. Cells, 12(1), 145.

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Immunofluorescence analysis of p21, c-Myc, and NF-κB

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FaDu cells were grown on slides, fixed in 4% paraformaldehyde in PBS for 15 min at RT and washed with PBS. Cells were permeabilized with 0.2% Triton X-100 in PBS for 10 min. Cells were blocked with 5% goat serum for 1 hour, and incubated with rabbit polyclonal anti-p21 (1:100; Bioss Antibodies), rabbit polyclonal anti-C-Myc (1:100; Bioss Antibodies), rabbit monoclonal anti-NFκB p65 (1:100, Abcam) overnight at 4°C. The next day cells were washed 3 times with PBS, and incubated with goat Alexa 555 conjugated anti-rabbit IgG (1:400, Abcam) for 1 hour at room temperature in the dark. Cells were mounted in 70% glycerol and images were taken by laser confocal microscopy (Fluo-View FV1000; Olympus, Japan).
Detection of the fluorescent intensity (FI) of FaDu cells stained with anti-p21 or anti-C-Myc antibodies were preformed under a laser scanning confocal microscope. Positive signals were analyzed as mean fluorescent intensity (MFI) using the FV10-ASW 4.0 software (Fluo-View FV1000; Olympus, Japan). In brief, 100 cells from each treatment group were analyzed in a blinded manner. All images were captured under the same camera settings.

Niu K., Guo C., Teng S., Zhou D., Yu S., Yin W., Wang P., Zhu W, & Duan M. (2020). Pepsin promotes laryngopharyngeal neoplasia by modulating signaling pathways to induce cell proliferation. PLoS ONE, 15(1), e0227408.

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