Levels of interferon alpha (IFNα) in serum were measured using IFNα ELISA kit (Cloud-Clone Corp., Houston, TX, USA) specific for rhesus monkeys in accordance with the manufacturer’s instructions. Results were detected by absorbance measurement at 450 nm on Multiscan Microplate Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Concentrations of IFNα were calculated relative to the calibration curve plotted using standard samples included in the kit.
Multiscan microplate spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Multiscan microplate spectrophotometer is a lab equipment product designed to measure the absorbance or optical density of samples in a microplate format. It can be used to quantify various biomolecules, assess cell viability, and perform other spectrophotometric analyses.
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6 protocols using multiscan microplate spectrophotometer
1
Quantification of Serum IFNα in Rhesus Monkeys
2
Caki-1 Cell Metabolic Activity Assay
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The metabolic activity of Caki-1 cells was assessed using CellTiter 96™ AQueous Nonradioactive Cell Proliferation Assay, according to the manufacturer’s instructions (Promega, Madison, WI, USA). The optical density of the wells was measured at 450 nm using a Multiscan microplate spectrophotometer (Thermo LabSystems, Milford, MA, USA).
3
Cell Proliferation Assay with CCK-8
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The cell proliferation capacity of siRNA-transfected cultures was determined using Cell Counting Kit-8 solution (Dojindo, Gaithersburg, Kumamoto, Japan) according to the manufacturer’s protocol. Briefly, cells were seeded at a concentration of 5 × 103 cells/100 μl/well in 96-well culture plates and treated with 10 μl/well of Cell Counting Kit-8H solution during the last 2 h of culturing. The optical density of the wells was measured at 450 nm using a Multiscan microplate spectrophotometer (Thermo LabSystems, Milford, MA).
4
Cell Proliferation Assay for 786-O and Caki-1
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The metabolic activity of 786-O and Caki-1 cells was assessed using CellTiter 96™ AQueous Nonradioactive Cell Proliferation Assay (Promega, WI, USA). The optical density of the wells was measured at 450 nm using a Multiscan microplate spectrophotometer (Thermo LabSystems, MA, USA).
5
Cell Proliferation Assay for 786-O and Caki-1
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The metabolic activity of 786-O and Caki-1 cells was assessed using CellTiter 96™ AQueous Nonradioactive Cell Proliferation Assay (Promega, WI, USA). The optical density of the wells was measured at 450 nm using a Multiscan microplate spectrophotometer (Thermo LabSystems, MA, USA).
6
In-vitro Cytotoxicity Assay for Compound Screening
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In-vitro cytotoxicity was performed as described previously by Chowdhury et al. 17 Briefly, cells were cultured in 96 well plates. After 24h, cells were treated with different treatments for specific period of time. Thereafter, MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) (SRL) was added to each treated and control well and incubated for 4h. Formazan crystals were solubilized in DMSO (dimethyl sulfoxide) and readings were obtained at 570nm with a differential filter of 630nm using Multiscan Microplate Spectrophotometer (Thermo Scientific). Percentage of viable cells was calculated using the following formula: Viability (%) = (mean absorbance value of drug-treated cells) / (mean absorbance value of control) ×100. A concentration of 0.2% DMSO was found to be non-toxic and was used for dissolving BMPP-Pt, and used as control in cytotoxicity experiments.
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