HepG2 cells were infected with Tg-PbANKA/LISP2 or WT PbANKA SPZ and cultured for 48 h. Supernatants were recovered and the amount of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
Cytokine specific elisa kits
Manufactured by BD
Cytokine-specific ELISA kits are laboratory equipment used to quantify the levels of specific cytokines in biological samples. These kits employ the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of target cytokines.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated goat anti mouse secondary antibody, by Cell Signaling Technology (1 mentions)
Nunc immuno plate, by Thermo Fisher Scientific (1 mentions)
O phenylenediamine dihydrochloride substrate, by Merck Group (1 mentions)
Ox ldl, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
4 protocols using cytokine specific elisa kits
1
Cytokine production in HepG2 cells
2
Parasite Antibody Detection ELISA Protocol
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To detect parasite-specific antibodies, 96-well plates (Nunc-immuno plate; Thermo Scientific, Rockford, IL) were coated with parasite protein extract from asexual blood stages in carbonate buffer, pH 9.6, for 2 h at 37 °C. After the plates were saturated with 1% (w/v) pork gelatine, each serum was assayed at serial dilutions and incubated overnight for 2 h at 37 °C. Specific binding was detected using HRP-conjugated goat anti-mouse secondary antibody (Cell Signalling technology®, Danvers, MA) followed by the addition of o-phenylenediamine dihydrochloride (OPD) substrate (Sigma-Aldrich; St.Louis, MO). Hydrogen chloride (HCl) 1 N was used to block the reaction. The optical density (OD) was read at 490–655 nm. Each sample was tested against non-immune serum and PBS as background controls. Amounts of IL-12p70, IFN-γ and IL-6 in the serum were analysed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
3
Cytokine and Biomarker Measurement
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TNF-α, IL-12 p40, and IFN-γ levels in the serum as well as IL-12 p40 and IL-10
levels in the culture medium were measured using the respective
cytokine-specific ELISA kits (BD Bioscience, San Diego, CA).21 (link),22 (link) CRP was
determined using ELISA Kit, Mouse (Kamiya Biomedical Company, Seattle, WA),
while ALT and glucose were measured using a DRI-CHEM 3500V system (Fuji Film,
Tokyo, Japan).
levels in the culture medium were measured using the respective
cytokine-specific ELISA kits (BD Bioscience, San Diego, CA).21 (link),22 (link) CRP was
determined using ELISA Kit, Mouse (Kamiya Biomedical Company, Seattle, WA),
while ALT and glucose were measured using a DRI-CHEM 3500V system (Fuji Film,
Tokyo, Japan).
4
LPS and oxLDL Stimulate Cytokine Secretion
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GFP-expressing RAW264.7 cells were stimulated by LPS (5 μg/ml, Sigma) or oxLDL (10 μg/ml) for 24 h, and secretion of IL-12 or TNF-α into the supernatant was analyzed using cytokine specific ELISA kits according to manufacturer instructions (BD Bioscience).
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