Avance 500 mhz

Manufactured by Agilent Technologies

The Avance 500 MHz is a high-performance nuclear magnetic resonance (NMR) spectrometer manufactured by Agilent Technologies. It operates at a frequency of 500 MHz and is designed to provide accurate and reliable analysis of various chemical compounds and materials.

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Lab products found in correlation

Synapt g1 mass spectrometer, by Waters Corporation (1 mentions) Inova 500 mhz, by Agilent Technologies (1 mentions) Vnmrs 500 mhz, by Agilent Technologies (1 mentions)

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Avance 500

2 protocols using avance 500 mhz

NMR and MS Characterization of Compounds

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Nuclear magnetic resonance (NMR) spectra were recorded in D₂O, CD₃OD, or CDCl₃ at 25°C using Bruker Avance 500 MHz, Varian Inova 500 MHz, and Agilent 600 MHz (3 mm cold probe) instruments, as indicated on the spectra in the Supplementary Figures S1–S4 and Supplementary Tables S1–S3. Spectra run in D₂O were not re-referenced. Spectra run in CD₃OD were referenced to the residual solvent peaks at 3.31 ppm and 49.0 ppm for ¹H and ¹³C NMR, respectively. Spectra run in CDCl₃ were referenced to residual solvent peaks at 7.25 and 77.0 ppm for for ¹H and ¹³C NMR, respectively. Accurate-mass MS data were recorded on a Waters Synapt G1 mass spectrometer after calibration with a sodium formate solution with a mass range from 100 to 2000 m/z. System suitability was obtained during each run, in which the difference between the calculated and observed m/z values were less than 5 ppm for each of four standard compounds, with m/z ranging from 152 to 609.

Stulberg E.R., Lozano G.L., Morin J.B., Park H., Baraban E.G., Mlot C., Heffelfinger C., Phillips G.M., Rush J.S., Phillips A.J., Broderick N.A., Thomas M.G., Stabb E.V, & Handelsman J. (2016). Genomic and Secondary Metabolite Analyses of Streptomyces sp. 2AW Provide Insight into the Evolution of the Cycloheximide Pathway. Frontiers in Microbiology, 7, 573.

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NMR Structural Characterization of Metalated α3DIV

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NMR experiments for the structure determination were performed at 25 °C on a Varian (Agilent) INOVA 800 MHz NMR spectrometer, equipped with a triple resonance cold probe. The NMR acquisition parameters for the 800 MHz spectrometer are reported in Supporting Information, Table 1. ¹⁵N-HSQC spectra were collected for the metalated α₃DIV species and their corresponding apo controls. These experiments were performed on a Bruker Avance 500 MHz with room temperature triple resonance probes and a Varian VNMRS 500 MHz equipped with a switchable probe. The N-HSQC experiments for the Pb(II)- N-α₃DIV and its apo control were collected at 25 °C, while the Hg(II)- N-α₃DIV and its apo control were obtained at 9 °C. The N-HSQC experiments were also performed at 25 °C for apo N-α₃DIV with pH conditions of 5.8 and 8.6. The spectra were processed using NMRPIPE^{24 (link)} and then visualized and analyzed with SPARKY.²⁵

Plegaria J.S., Dzul S.P., Zuiderweg E.R., Stemmler T.L, & Pecoraro V.L. (2015). Apoprotein Structure and Metal Binding Characterization of a de Novo Designed Peptide, α3DIV, that Sequesters Toxic Heavy Metals. Biochemistry, 54(18), 2858-2873.

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