Antibodies against β-Actin (AC026, 1:10000), NUDT1 (A13330, 1:1000 for immunoblots, 1:200 for immunoprecipitation), NOX4 (A11274, 1:1000), H3 (A2348, 1:1000), NCL (A5904, 1:1000) were obtained from ABclonal. PLK1 (4513 S, 1:1000), Phospho-PLK1 (Thr210) (5472 T, 1:1000), MYC (13987, 1:1000 for immunoblots), cleaved-Caspase 3 (Asp175) (9661 S, 1:1000 for immunoblots, 1:100 for IHC) were obtained from Cell Signaling technology. Flag-tag (F1804, 1 μg for immunoprecipitation) was obtained from Sigma–Aldrich. N-MYC (sc-53993, 1:1000 for immunoblots, 4 μg for ChIP) and PCNA (sc-56, 1:2000 for IHC) were obtained from Santa Cruz Biotechnology. 8-oxo-dG (AB5830, 1:300 for IHC) and γH2AX (05-636, 1:200 for IHC, 1:100 for IF, 1:1000 for immunoblots) were obtained from Millipore. HRP-conjugated goat anti-mouse (115-035-003, 1:5000) and anti-rabbit (111-035-003, 1:5000) secondary antibodies were obtained from Jackson ImmunoResearch Laboratories. The Phospho-NUDT1 S121 antibody was generated by Dia-An Technology by immunizing rabbits with phosphorylated S121 peptides (PDD-(phospho)S-YWF) conjugated with carrier protein keyhole limpet hemacyanin.
Other chemicals include 4-OH Tamoxifen, Tetracycline HCl, and TH287 (Selleck Chemicals); Acetylcysteine, BI6727, MG132, Pomalidomide and SBE-β-CD (MCE); CHX (Sigma–Aldrich); DAPI (Yeasen); Alexa488-conjugated avidin (Invitrogen).
Other chemicals include 4-OH Tamoxifen, Tetracycline HCl, and TH287 (Selleck Chemicals); Acetylcysteine, BI6727, MG132, Pomalidomide and SBE-β-CD (MCE); CHX (Sigma–Aldrich); DAPI (Yeasen); Alexa488-conjugated avidin (Invitrogen).