Total RNA was isolated from human breast cancer cells (Non-silenced and LASP-1 KD cells) cultured on 3D-Matrigel using the RNA-STAT 60 kit (Amsbio, Lake Forest, CA). The quality of the total RNA was tested at the Vanderbilt VANTAGE core and the RNA integrity number (RIN value) ranged from 9.6–10 (high quality). Total RNA with high RIN values and 28S:18S ratio was subjected to microarray analysis. The changes in gene expression upon knock down of LASP-1were analyzed with Affymetrix human Gene 1.0 ST and 2.0 ST arrays (Affymetrix, Santa Clara, CA). The microarray experiments were performed in 2–4 biological replicates. The results were analyzed by PARTEK Genomics software.
Human gene 1.0 st and 2 0 st arrays
Manufactured by Thermo Fisher Scientific
The Human Gene 1.0 ST and 2.0 ST arrays are high-density oligonucleotide microarray products designed for the analysis of the human transcriptome. These arrays provide comprehensive coverage of protein-coding and non-coding transcripts, allowing for the measurement of gene expression levels across the entire genome.
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Lab products found in correlation
2 protocols using human gene 1.0 st and 2 0 st arrays
1
Transcriptome analysis of LASP-1 knockdown
2
Transcriptome analysis of LASP-1 knockdown
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Total RNA was isolated from human breast cancer cells (Non-silenced and LASP-1 KD cells) cultured on 3D-Matrigel using the RNA-STAT 60 kit (Amsbio, Lake Forest, CA). The quality of the total RNA was tested at the Vanderbilt VANTAGE core and the RNA integrity number (RIN value) ranged from 9.6–10 (high quality). Total RNA with high RIN values and 28S:18S ratio was subjected to microarray analysis. The changes in gene expression upon knock down of LASP-1were analyzed with Affymetrix human Gene 1.0 ST and 2.0 ST arrays (Affymetrix, Santa Clara, CA). The microarray experiments were performed in 2–4 biological replicates. The results were analyzed by PARTEK Genomics software.
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