Gamma secretase inhibitor (GSI,MRK-003; Merck) was made fresh weekly and dosed at 300 mg/kg in 0.5% methocel by oral gavage (Lewis et al., 2007 (link); Sparey et al., 2005 (link)). For pathology, we dosed mice once weekly for 4 weeks and sacrificed 6 hr after the last dose (n = 5 doses). For flow analysis, we dosed mice once weekly for 1 week and sacrificed mice 6 hr after the last dose (n = 2 doses). Fresh solution of L-NAME (L-NG-nitroarginine methyl ester, 100 μM in 1× PBS; Sigma-Aldrich) was administered daily at 0.4 mg/kg. For pathology and flow analysis, mice were injected intraperitoneally (i.p.) daily for 7 days and sacrificed 6 hr after the last dose. MEK inhibitor (PD0325901; Pfizer) was made fresh weekly and dosed at 1.5 mg/kg/day in 0.5% methocel/0.2% Tween 80, by oral gavage. For pathology, we dosed mice every day for 3 weeks. For flow analysis, we dosed mice every day for 7 days and sacrificed 6 hr after the last dose.
Mek inhibitor
Manufactured by Pfizer
The MEK inhibitor is a laboratory product that functions to inhibit the activity of the MEK enzyme. MEK is a key component of the MAPK/ERK signaling pathway, which plays a role in cellular processes such as proliferation, differentiation, and survival. The MEK inhibitor can be used in research settings to study the effects of MEK inhibition on various biological systems.
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2 protocols using mek inhibitor
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Gamma Secretase Inhibitor Dosing Protocol
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Gamma Secretase Inhibitor Dosing Protocol
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Gamma secretase inhibitor (GSI,MRK-003; Merck) was made fresh weekly and dosed at 300 mg/kg in 0.5% methocel by oral gavage (Lewis et al., 2007 (link); Sparey et al., 2005 (link)). For pathology, we dosed mice once weekly for 4 weeks and sacrificed 6 hr after the last dose (n = 5 doses). For flow analysis, we dosed mice once weekly for 1 week and sacrificed mice 6 hr after the last dose (n = 2 doses). Fresh solution of L-NAME (L-NG-nitroarginine methyl ester, 100 μM in 1× PBS; Sigma-Aldrich) was administered daily at 0.4 mg/kg. For pathology and flow analysis, mice were injected intraperitoneally (i.p.) daily for 7 days and sacrificed 6 hr after the last dose. MEK inhibitor (PD0325901; Pfizer) was made fresh weekly and dosed at 1.5 mg/kg/day in 0.5% methocel/0.2% Tween 80, by oral gavage. For pathology, we dosed mice every day for 3 weeks. For flow analysis, we dosed mice every day for 7 days and sacrificed 6 hr after the last dose.
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