Equal amounts of protein (30 μg) were separated by electrophoresis in NuPAGE Bis-Tris Gels (Life Technologies) and transferred to nitrocellulose membranes. The membranes were hybridized with various primary antibodies (APP 6E10, 1/500, Covance; PS1, 1/1000, Millipore; APP C-ter, 1/500, Millipore; Actin, 1/2000, Abcam; GAPDH, 1/1000 Abcam; Total Tau, 1/1000, Santa Cruz; PSD-95, Invitrogen, 1/2000; Synaptophysin, Santa Cruz, 1/200; GAD65, Abcam, 1/2000; GLT-1, 1/1000, Frontier Science; GLAST, 1/1000, Frontier Science; NeuN, Millipore, 1/1000). Various secondary antibodies was also used (ECL Anti-rabbit Horseradish Peroxidase linked, 1/2000, GE Healthcare; ECL Anti-mouse Horseradish Peroxidase linked, 1/2000, GE Healthcare; ECL Anti-rat Horseradish Peroxidase linked, 1/2000, GE Healthcare).
Ecl anti rabbit horseradish peroxidase linked
Manufactured by GE Healthcare
ECL Anti-rabbit Horseradish Peroxidase linked is a reagent used in immunodetection techniques. It contains a horseradish peroxidase-conjugated antibody specific to rabbit immunoglobulins. This reagent can be used to detect and visualize rabbit primary antibodies in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions)
Gapdh, by Abcam (2 mentions)
Synaptophysin, by Santa Cruz Biotechnology (1 mentions)
App c ter, by Merck Group (1 mentions)
Total tau, by Santa Cruz Biotechnology (1 mentions)
Psd 95, by Thermo Fisher Scientific (1 mentions)
Gad65, by Abcam (1 mentions)
Neuronal nuclei (neun), by Merck Group (1 mentions)
App 6e10, by Fortrea (1 mentions)
Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions)
Trem2, by R&D Systems (1 mentions)
Dyrk1a 7d10, by Abnova (1 mentions)
Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions)
P stat3 y705, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Vimentin, by Abcam (1 mentions)
Fusion fx7, by Vilber (1 mentions)
2 protocols using ecl anti rabbit horseradish peroxidase linked
1
Western Blot Analysis of Neurological Proteins
2
Quantitative Western Blot Analysis
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Equal amounts of protein (30 μg) were separated by electrophoresis in NuPAGE Bis-Tris Gels (Life Technologies) and transferred to nitrocellulose membranes. The membranes were hybridized with various primary antibodies (DYRK1A 7D10 (1/250, Abnova), DYRK1A D1694 (1/500, Sigma), GFAP (DAKO, 1/4000), Vimentin (1/1000, Abcam), pSTAT3(Y705) (1/1000, Cell Signaling), STAT3 (1/1000, Cell Signaling), IBA1 (1/2000, Wako), CD68 (1/1000, BioLegend), IDE (1/200, Santa Cruz), TREM2 (R&D Systems, 1/500), GAPDH (1/1000, Abcam)). Various secondary antibodies were also used (ECL Anti-mouse horseradish peroxidase linked, 1/2000, GE Healthcare; ECL Anti-rabbit horseradish peroxidase linked, 1/2000, GE Healthcare). Membranes were developed using enhanced chemiluminescence (Thermo Fisher Scientific). Signals were detected with Fusion FX7 (Vilber Lourmat) and analyzed and quantified using ImageJ (NIH).
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