After enucleation, the lens and cornea were removed and each eye cup incubated in 4% PFA, incubated in a 30% sucrose solution before being frozen in OCT compound (VWR, Lutterworth, UK). Eyes were sectioned and dried before permeabilizing with 0.2% Triton-X 100. Slides were washed in PBS before blocking with 10% bovine serum albumin and 10% donkey serum. Slides were washed before primary antibody incubation (goat polyclonal, ABIN343052, AntibodiesOnline [Aachen Germany], used 1/200 in 1% bovine serum albumin, 1% serum) and secondary antibody incubation (donkey anti-goat 488, ab1050129 [Abcam, Cambridge, UK] used 1/400 in 1% bovine serum albumin and 1% serum). Hoescht stain was performed (Sigma-Aldrich, Gillingham, UK) (1/2,000 in PBS) before a final wash in PBS. ProLong Diamond antifade mounting medium (Life Technologies Ltd, Paisley, UK) was applied and slides were sealed before imaging.
Abin343052
Manufactured by Antibodies-online
Sourced in Germany
The ABIN343052 is a lab equipment product. It is designed for use in laboratory settings. The core function of this product is to perform a specific task or procedure required in a laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using abin343052
1
Immunohistochemical Analysis of Ocular Tissues
2
Immunohistochemical Analysis of Ocular Tissues
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After enucleation, the lens and cornea were removed and each eye cup incubated in 4% PFA, incubated in a 30% sucrose solution before being frozen in OCT compound (VWR, Lutterworth, UK). Eyes were sectioned and dried before permeabilizing with 0.2% Triton-X 100. Slides were washed in PBS before blocking with 10% bovine serum albumin and 10% donkey serum. Slides were washed before primary antibody incubation (goat polyclonal, ABIN343052, AntibodiesOnline [Aachen Germany], used 1/200 in 1% bovine serum albumin, 1% serum) and secondary antibody incubation (donkey anti-goat 488, ab1050129 [Abcam, Cambridge, UK] used 1/400 in 1% bovine serum albumin and 1% serum). Hoescht stain was performed (Sigma-Aldrich, Gillingham, UK) (1/2,000 in PBS) before a final wash in PBS. ProLong Diamond antifade mounting medium (Life Technologies Ltd, Paisley, UK) was applied and slides were sealed before imaging.
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