Hb 8064

Manufactured by American Type Culture Collection

Sourced in United States

The HB-8064 is a laboratory equipment product designed for cell culture applications. It is a high-performance incubator that provides a controlled environment for the growth and maintenance of cell lines. The product specifications and technical details are available upon request.

Automatically generated - may contain errors

Lab products found in correlation

27 protocols using hb 8064

Xenograft Models for Evaluating Anti-Cancer Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 14

Various human cancer cells, such as human hepatocellular carcinoma Hep3B cells (ATCC #HB-8064) and human colorectal cancer LoVo cells (ATCC #CCL-229), can be used to establish xenograft models in nude mice. In order to assess the inhibitory effects of ACPs and ACVPs on tumor growth, various concentrations of each ACP and each ACVP (e.g., from 0.1-10 mg/kg) is administered twice weekly intravenously in the mice after tumor cell implantation. Tumor growth is measured weekly up to 7 weeks.

US11518984B2. Protein inhibitors to complement and VEGF pathways and methods of use thereof (2022-12-06). AP Biosciences, Inc. [TW], Innovent Biologics, Inc. [KY]. Inventors: Jeng-Horng Her [US], Huang-Tsu Chen [US].

+ Open protocol

+ Expand

Cell Culture and Antibody Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Hep3B2.1–7 (Hep3B) cell line was obtained from ATCC (HB-8064; ATCC, Manassas, VA); the HuH-7 cell line was from the laboratory of Dr. Greg Gores (Mayo Clinic, Rochester, MN). The Rab10 knockout fibroblasts were supplied by the laboratory of David James (University of Sydney, Australia). Cells were maintained in minimum essential medium (MEM) media containing 10% fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 μg/mL of streptomycin (Life Technologies, Carlsbad, CA), and grown at 37°C in 5% CO₂.
The rabbit Rab10 antibody was from Sigma-Aldrich (R8906; St. Louis, MO). The Rab10(4E2) antibody was from GeneTex (GTX82800; Irvine, CA). Dyn2 antibodies were prepared as detailed.^{(9 (link))} Alexa Fluor secondary antibodies were used for immunofluorescence along with ProLong Antifade reagent (Thermo Fisher Scientific, Waltham, MA). Horseradish peroxidase (HRP) secondary antibodies (Invitrogen) for western blotting analysis, detected using SuperSignal West Pico substrate (Thermo Fisher Scientific). Diacylglycerol O-acyltransferase (DGAT) inhibitors, were purchased from Sigma-Aldrich.

Li Z., Weller S.G., Drizyte-Miller K., Chen J., Krueger E.W., Mehall B., Stöckli J., Casey C.A., Cao H, & McNiven M.A. (2020). Maturation of Lipophagic Organelles in Hepatocytes Is Dependent Upon a Rab10/Dynamin-2 Complex. Hepatology (Baltimore, Md.), 72(2), 486-502.

+ Open protocol

+ Expand

Cell Culture Protocols for Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hep3b [American Type Culture Collection (ATCC) HB-8064], SK-HEP-1 (ATCC HTB-52), PC-3 (ATCC CRL-1435), and THLE-3 (ATCC CRL-11233) cells were purchased from ATCC (Manassas, VA). Huh7 was provided by P. Tran’s laboratory from the Johns Hopkins University School of Medicine. Hep3b and SK-HEP-1 cells were cultured in minimal essential medium (MEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, 100 μM MEM nonessential amino acids solution, and 1 mM sodium pyruvate. Huh7 and PC-3 cells were cultured in high-glucose Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. THLE-3 cells were cultured in bronchial epithelial cell growth medium (BEBM) with the additives from the kit [BEGM Bullet Kit (CC3170), Lonza/Clonetics Corp., Walkersville, MD], except gentamycin-amphotericin and epinephrine, 10% FBS, and 1% penicillin-streptomycin. For THLE-3 culture, flasks and plates were coated with 0.01 mg/ml of human fibronectin, 0.03 mg/ml of bovine collagen type 1, and 0.01 mg/ml of bovine serum albumin dissolved in BEBM basal medium and incubated overnight at 37°C. Coating solution was aspirated before seeding. THLE-3 cells were not used beyond passage 5. Cell cultures were maintained in a humidified incubator, at 37°C, with 5% CO₂.

Vaughan H.J., Zamboni C.G., Hassan L.F., Radant N.P., Jacob D., Mease R.C., Minn I., Tzeng S.Y., Gabrielson K.L., Bhardwaj P., Guo X., Francisco D., Pomper M.G, & Green J.J. (2022). Polymeric nanoparticles for dual-targeted theranostic gene delivery to hepatocellular carcinoma. Science Advances, 8(29), eabo6406.

+ Open protocol

+ Expand

Xenograft Tumor Growth Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 14

Various human cancer cells, such as human hepatocellular carcinoma Hep3B cells (ATCC# HB-8064) and human colorectal cancer LoVo cells (ATCC# CCL-229), can be used to establish xenograft models in nude mice. In order to assess the inhibitory effects of ACPs and ACVPs on tumor growth, various concentrations of each ACP and each ACVP (e.g., from 0.1-10 mg/kg) is administered twice weekly intravenously in the mice after tumor cell implantation. Tumor growth is measured weekly up to 7 weeks.

US09988611B2. Protein inhibitors to complement and VEGF pathways and methods of use thereof (2018-06-05). AP Biosciences, Inc. [TW], Innovent Biologies, Inc. [KY]. Inventors: Jeng-Horng Her [US], Huang-Tsu Chen [US].

+ Open protocol

+ Expand

Hep3B Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hep3B cells (ATCC^® HB-8064) were cultured in a 6-well plate and treated with 100 μL media containing 20 μM EdU. After continuous incubation at 37° C with 5% CO₂ for 24 h and 48 h, the cells were fixed with 4% paraformaldehyde for 15 min and incubated with 0.5% Triton X-100 in phosphate-buffered saline (PBS) for 10–15 min. Fluorescence microscopy was employed to acquire and analyze the images. All the experiments were performed at least three times, and the data were represented as mean ± standard deviation (SD).

Zhao Y., Yang B., Chen D., Zhou X., Wang M., Jiang J., Wei L, & Chen Z. (2021). Combined identification of ARID1A, CSMD1, and SENP3 as effective prognostic biomarkers for hepatocellular carcinoma. Aging (Albany NY), 13(3), 4696-4712.

+ Open protocol

+ Expand

Isolation and Characterization of CD133-Positive Liver and Lung Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

A human hepatoma cell line (Huh7, PTA-4583) and a transformed liver cell line (Hep3B, HB-8064) were obtained from ATCC (Manassas, VA). The cells were cultured in growth medium [Dulbecco’s modified Eagle’s medium (DMEM; Sigma‒Aldrich: St. Louis, MO) supplemented with 10% fetal bovine serum (FBS; GeminiBio, West Sacramento, CA), 0.1% nonessential amino acids (Gibco-BRL), 1% Glutamax-1 (Gibco-BRL, Grand Island, NY), and 1% antibiotic-antimycotic solution (Thermo Fisher Scientific, Irwindale, CA)]. Human lung cancer cell lines [Calu3 (HTB-55) and H1299 (CRL-5803)] were obtained from ATCC. These cultured cells were maintained in Eagle’s minimum essential medium (Gibco-BRL) supplemented with 1% Glutamax-1, 1% P/S, and 10% FBS. CD133-positive/negative Huh7, Hep3B, and H1299 cells were isolated using CD133-conjugated magnetic microbeads (AC133, Cell Isolation Kit, Miltenyi Biotec, Bergisch Gladbach, Germany). Two rounds of magnetic separation were performed. CD133-positive cells were cultured in growth medium as described above. The isolation quality was controlled by flow cytometry with an antibody against a different CD133 epitope (Santa Cruz Biotechnology, Dallas, TX).

Choi H.Y., Zhu Y., Zhao X., Mehta S., Hernandez J.C., Lee J.J., Kou Y., Machida R., Giacca M., Del Sal G., Ray R., Eoh H., Tahara S.M., Chen L., Tsukamoto H, & Machida K. (2024). NOTCH localizes to mitochondria through the TBC1D15-FIS1 interaction and is stabilized via blockade of E3 ligase and CDK8 recruitment to reprogram tumor-initiating cells. Experimental & Molecular Medicine, 56(2), 461-477.

+ Open protocol

+ Expand

Photocaged Dihydrotetrazine Cytotoxicity in Hep 3B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human Hep 3B hepatocellular carcinoma cells (ATCC, HB-8064) were grown in the complete medium Eagle’s minimal essential medium (EMEM) with high glucose (Life Technologies–Gibco, 11995073) supplemented with 10% heat-inactivated FBS (Omega Scientific, FB02), 100 U ml⁻¹ penicillin and 100 μg ml⁻¹ streptomycin (Life Technologies–Gibco, 15140122). Cells were grown at 37 °C in a humidified atmosphere containing 5% CO₂. Hep 3B cell lines were plated at 30,000 cells per well on 96-well plates in 200 μl of EMEM complete medium and incubated for 24 h to promote cell adhesion and growth. The cells were then treated, respectively, with photocaged dihydrotetrazine 1c (8 μM), TCO-Dox 3c (5.5 μM), side product 4c (5.5 μM) and Dox 5a (5.5 μM), with or without irradiation by LED light (450 nm, 18 W) for 2 min, followed by incubation at 37 °C for 24 h. Cell viability assays of Hep 3B cancer cells were carried out next.
ATCC has profiled cell lines using polymorphic short tandem repeat (STR) loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818) plus amelogenin for gender identification.

Kolb-Mäurer A., Pilgrim S., Kämpgen E., McLellan A.D., Bröcker E.B., Goebel W, & Gentschev I. (2001). Antibodies against Listerial Protein 60 Act as an Opsonin for Phagocytosis of Listeria monocytogenes by Human Dendritic Cells. Infection and Immunity, 69(5), 3100-3109.

+ Open protocol

+ Expand

Cell Culture Protocols for HEK293T and HEP3B

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T/17 cells (sex: female) were obtained from ATCC (catalog CRL-11268), maintained in DMEM, and supplemented with sodium pyruvate, 10% heat-inactivated fetal bovine serum, and glutamine. HEP3B cells (sex: male) were obtained from ATCC (catalog HB-8064) and maintained in modified eagle medium supplemented with sodium pyruvate, 10% heat-inactivated fetal bovine serum, nonessential amino acids, penicillin-streptomycin, and glutamine. Cells were cultured at 37°C in a humidified incubator with 5% CO₂, and monolayers were disrupted at 80% to 100% confluence with Trypsin-EDTA.

Frumento N., Figueroa A., Wang T., Zahid M.N., Wang S., Massaccesi G., Stavrakis G., Crowe JE J.r., Flyak A.I., Ji H., Ray S.C., Shaw G.M., Cox A.L, & Bailey J.R. (2022). Repeated exposure to heterologous hepatitis C viruses associates with enhanced neutralizing antibody breadth and potency. The Journal of Clinical Investigation, 132(15), e160058.

+ Open protocol

+ Expand

ER Stress Response in Liver Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HepG2 (HB-8065; ATCC, Virginia, USA), Hep3B (HB-8064; ATCC) and Huh7 (kindly provided by Dr. Olivier Govaere (University of Leuven, Belgium)) cells were cultured in DMEM supplemented with 10 % foetal bovine serum (Life Technologies, Ghent, Belgium). None of the three cell lines used require ethical approval. Cells were incubated for 24 h or 48 h with a PERK inhibitor (0.3 μM; GSK2656157, NoVi Biotechnology, Shandong, China), an IRE1 inhibitor (8 μM; 4μ8C, Calbiochem, Massachusetts, USA), tauroursodeoxycholic acid (TUDCA, 1 mM), tunicamycin (1 μM), thapsigargin (150 nM) or quercetin (100–300 μM) and compared to equal volumes of solvent. All reagents were obtained from Sigma (Diegem, Belgium) unless stated otherwise. Hypoxic atmosphere (1 % oxygen) was established in a hypoxic chamber (AnaeroGen; Oxoid, Basingstoke, UK). Experiments were carried out in quadruplicate and independently repeated three times.
Detailed information regarding total RNA extraction, quantitative real-time PCR, Western blotting, and immunohistochemistry is provided in the Additional file 1.

Vandewynckel Y.P., Laukens D., Devisscher L., Bogaerts E., Paridaens A., Van den Bussche A., Raevens S., Verhelst X., Van Steenkiste C., Jonckx B., Libbrecht L., Geerts A., Carmeliet P, & Van Vlierberghe H. (2016). Placental growth factor inhibition modulates the interplay between hypoxia and unfolded protein response in hepatocellular carcinoma. BMC Cancer, 16, 9.

+ Open protocol

+ Expand

Culturing Hepatocellular Carcinoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hep3B (hepatocellular carcinoma, ATCC® HB-8064), HepG2 (hepatoblastoma, ATCC® HB-8065) and SK-Hep1 (hepatocellular adenocarcinoma, ATCC® HTB-52) cells were purchased form the American Type Culture Collection (ATCC, Manassas, VA, USA). Hep3B and SK-Hep1 were cultured in Dulbecco’s Modified Eagle’s Medium (D MEM; Gibco by Life Technologies) supplemented with 4.5 g/L D-glucose, 10% heat-inactivated FBS, 2 mM L-glutamine (Gibco by Life Technologies), 1 mM sodium pyruvate (Gibco by Life Technologies) and 10 mM HEPES (Gibco by Life Technologies). HepG2 was cultured in Minimum Essential Medium (MEM; Gibco by Life Technologies) supplemented with heat-inactivated 10% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate and 10 mM HEPES. The cells were maintained in a humidified atmosphere of 5% CO₂ and 95% air at 37 °C.

Choi J.W., Lee E.S., Kim S.Y., Park S.I., Oh S., Kang J.H., Ryu H.A, & Lee S. (2019). Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer. BMC Cancer, 19, 817.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!