Two digital acousto-optical deflectors (DTSXY-400-1064; AA Opto Electronic, Orsay, France) control the x-y position of the laser in a custom-built setup (see Fig. S4 ). They are used to create two optical traps in time-sharing mode at a switching rate of 20 kHz using a digital controller (DDSPA2X-D423b-34-0dB; AA Optoelectronics). The continuous wave IR-laser beam (YLM-1-1064-LP; IPG Photonics, Oxford, MA) is widened to slightly overfill the back aperture of the focusing objective (UPLSAPO60XW/IR, NA 1.2, 60×, water immersion; Olympus, Melville, NY) and the light is collected by a long distance objective (60×, water immersion, LUMPLFL 60×, NA 0.9; Olympus). A four-quadrant photodiode (QPD; Cat. No. G6849; Hamamatsu, Hamamatsu City, Japan) is connected to an amplifier (Öffner MSR-Technik, Plankstadt, Germany) to detect the position of trapped beads. Two controllers (PCIe-6259 and NI PCIe-6363; National Instruments, Austin, TX) are used for data acquisition and setup control, allowing a sampling rate of 500 kHz per channel of the QPD. The control of the setup and the analysis of the data are realized using the softwares LabVIEW 2010 (National Instruments) and MATLAB 2011b (The MathWorks, Natick, MA).
Ni pcie 6363
Manufactured by National Instruments
Sourced in United States
The NI PCIe-6363 is a high-performance data acquisition (DAQ) device that provides a PCIe interface for data acquisition and control applications. It offers 32 single-ended or 16 differential analog input channels, four analog output channels, and 48 digital I/O lines.
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2 protocols using ni pcie 6363
1
Dual-trap Optical Tweezers with Acousto-Optical Control
2
Optogenetic Gamma Oscillation Induction
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Optogenetic stimulation was used to measure evoked EEG power in the gamma frequency band. The computer’s digital signal was converted into an analog signal for optogenetic stimulation, through a NI-DAQ (NI PCIe-6363; NI BNC-2110, National Instruments, Austin, TX, USA). To drive the laser light stimulation, a simultaneous input was provided to a 473 nm DPSS Laser (Cat. No. 21-01311-05, CNI Laser, Changchun, China). The laser and an optical fiber on the head of a mouse were connected through a mono fiber patch cord (MFP_2m_FC-ZF1.25; 0.22 NA, 200 µm core, Doric Lenses, Quebec, QC, Canada). Using WinWCP software, we made a stimulation protocol. To trigger a gamma signal at 40 Hz, a pulse occurs every 25 ms for 0.5 s, and each pulse has a duration of 10 ms. There were 0.5 s of rest time before and after the stimulation periods. Our protocol gave 200 trains using this 1.5 s train (0.5 s pre-stimulation, 0.5 s stimulation, 0.5 s post-stimulation).
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