To assess the capacity of proliferation, the BMSCs were evenly seeded in six-well plates with 2 mL culture medium. The medium was refreshed every 3 days. After culturing for 14 days, the cells were washed with phosphate buffered saline (PBS) (Beyotime, China), fixed in 4% paraformaldehyde (Solarbio, China), and stained with 1% crystal violet staining solution (Biosharp, China) for 15 min. Subsequently, the cells were rinsed with double distilled water three times. Finally, pictures were taken under an inverted microscope (Nikon, Japan).
Crystal violet staining solution
Manufactured by Biosharp
Sourced in China
Crystal violet staining solution is a common laboratory reagent used for the visualization and identification of bacteria. It is a purple dye that binds to the cell walls of gram-positive bacteria, staining them purple. The solution is typically used in the Gram staining procedure, which is a widely-used technique for the classification of bacteria based on their cell wall structure.
Automatically generated - may contain errors
3 protocols using crystal violet staining solution
1
Quantifying BMSC Proliferation Capacity
2
Cell Migration Assay with ECM
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In brief, 200 μL cells (105 cells/mL) that were resuspended with a free medium were added to the upper compartment of the migration chambers (BD Biosciences, San Jose, CA). The bottom chamber was filled with 500 μL cell culture ECM with 5% FBS as an attractant. After 24 hours, cells were fixed and stained with Crystal Violet Staining Solution (BL802A; Biosharp) and counted under microscopic observation.
3
Chondrocyte Transfection and Staining
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Chondrocytes were inoculated into a six-well plate, transfected with miR-204 mimics or inhibitor, and cultured for 14 days. Then the cells were washed three times with PBS, fixed with 4% paraformaldehyde (Solar Bio, China) for 30 min, and incubated with 1 mL of crystal violet staining solution (Biosharp, China) for 30 min. After washed three times, the cells were observed under an optical microscope (Nikon, Japan) and photographed.
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