The AAV-GFP virus contained both AAV serotype 2 capsid and expressed GFP. GFP expression which was expressed under the control of the cytomegalovirus (CMV) immediate-early promoter (AAV2-GFP, 1.0 × 1013 viral particles/ml; Vector BioLabs, Philadelphia, USA) was used as a control. Mouse KLF7 was subcloned into an AAV2 vector cassette under the control of the CMV (AAV-m-KLF7; Vector BioLabs, Philadelphia, USA).
Aav2 gfp
Manufactured by Vector Biolabs
Sourced in United States
AAV2-GFP is a recombinant adeno-associated virus (AAV) vector containing the green fluorescent protein (GFP) gene. It is designed for gene delivery and expression studies.
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Lab products found in correlation
9 protocols using aav2 gfp
1
AAV-Mediated Overexpression of KLF7
2
Optic Nerve Labeling in Ank3 Mice
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AAV2-Cre-GFP or AAV2-GFP (Vector Biolabs) was injected into the eyes of P21 Ank3F/F mice using a pulled glass needle. Both Cre and GFP were driven by their own CMV promoters. The optic nerves were dissected 2 months after virus injection, followed by immunostaining.
3
Viral-Mediated Fmr1 Knockdown in NAc
4
AAV-mediated gene delivery to VMH
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Adeno-associated virus (AAV) vectors expressing Cre-GFP (category no. 7016, AAV2-Cre-GFP), Lin28a-GFP (AAV2-CMV-GFP-CMV-mLin28a, customized virus), and GFP (category no. 7004, AAV2-GFP) were purchased from Vector Biolabs and injected bilaterally into the VMH (coordinates, bregma: anterior-posterior, −1.5 mm; lateral, ±0.4 mm; and dorsal-ventral, −5.8 mm) at a rate of 40 nL/min (∼1 × 1012 viral particles/mL) for 15 min, and the injector remained in place for an additional 5 min before removal.
5
Targeted Cre expression in VTA
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Adult male and female floxed Gabrd and Gabrg2 mice were stereotaxically injected with either AAV2-GFP or AAV2-Cre-GFP (Vector Biolabs) into the VTA using the following coordinates: A/P −3.6 mm, M/L ±0.5 mm, D/V 4.5 mm. This approach results in expression of GFP or Cre recombinase in a subset of neurons in the VTA.
6
Stereotaxic Viral Vector Delivery to Dorsal Hippocampus
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Mice were anesthetized by intraperitoneal (i.p.) injection of a ketamine-xylazine mixture (100 mg/kg ketamine, 10 mg/kg xylazine). AAV2-GFP (Vector BioLabs #7004), AAV8-hSyn-DIO-hM3D(Gq)-mCherry (UNC Vectore Core), AAV8-hSyn-DIO-hM4D(Gi)-mCherry (UNC Vector Core), or AAV-FLEX-DTA (CMV-β-globin-DIO-mCherry-DTA-hGH pA generated by Dr. Patrick M. Fuller, Harvard Medical School) was stereotaxically injected into the dorsal hippocampus of each hemisphere (500 nL; posterior 2.0mm, lateral 1.5mm, depth 1.5mm) and the syringe was left in place for 5 minutes before withdrawal. For the EEG studies, mice were outfitted with an EEG/EMG headmount (Pinnacle #8201) utilizing stainless steel screws as two EEG leads, a reference electrode, EMG leads, and an animal ground. All animals were treated with buprenorphine (0.5mg/kg) preoperatively and then administered as needed to alleviate pain and/or distress to the animals during the three day postoperative observation period. Posthoc analysis was performed for each experiment to validate targeting and verify expected manipulations.
7
Optic Nerve Labeling in Ank3 Mice
8
AAV2-GFP Production and Delivery
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Recombinant adeno-associated virus 2 carrying eGFP (AAV2-GFP) with CMV-enhanced chicken b-actin (CAG) promoter was purchased from Vector Biolabs (Malvern, PA; stock #7072) or generated generated by helper virus-free system. 79 (link) 293T cells were grown to 70-80% confluence at which point they were transfected with two packaging plasmids using PEI (Polyethylenimine, linear, MW-25k, Warrington, PA): one carrying the AAV rep, cap genes and another helper plasmid carrying the adenovirus helper functions. Three days after transfection, the cell lysates and the supernatant were harvested and 40% PEG 8000 was added to precipitate crude virus for 2 h. The viruses were then purified by double-centrifugation with cesium chloride and the isolated virus was dialyzed in 0.1M PBS/5% sorbital overnight. AAV2-GFP was microinjected into a DRG using a micropipette pulled to a diameter of 0.05 mm and a nanoinjector (B203XVY Nanoliter 2000; World Precision Instruments, Sarasota, FL). For each injection, the micropipette was introduced 0.5 mm into the DRG and a total volume of AAV (1 mL/DRG) containing >1 3 10 13 GC/mL injected over a 10 min period. The glass needle was left in place for 5 min after each injection.
9
Adeno-Associated Virus Delivery of BDNF
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AAV1/2-IRES-CMV-BDNF-GFP (AAV-BDNF; 1 x 10 12 gc/ml) and AAV1/2-IRES-CMV-GFP (AAV-GFP; 1 x 10 12 gc/ml) were designed as described in [16] and were purchased from the University of North Carolina Viral Vector Core. AAV2-CMV-GFP-mBDNF (AAV2-BDNF; 7.7 x 10 12 gc/ml) and AAV2-CMV-GFP (AAV2-GFP; 1 x 10 13 gc/ml) were purchased from Vector Biolabs (Malverna, PA). AAV-DIO-Ef1a-BDNF-IRES-mCherry (AAV-DIO-BDNF-mCherry; 1 x 10 12 gc/ml) and AAV-DIO-Ef1a -mCherry (AAV-DIO-mCherry; 1 x 10 12 gc/ml) were constructed in conjunction with C&M Biolabs
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