Dl lacz

Manufactured by Bloomington Drosophila Stock Center

The Dl-LacZ is a reporter construct that contains the lacZ gene under the control of the Drosophila Distal-less (Dl) enhancer region. This construct is used to visualize the expression pattern of the Dl gene during Drosophila development.

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Lab products found in correlation

Delta lacz, by Bloomington Drosophila Stock Center (2 mentions) Uas nlsgfp, by Bloomington Drosophila Stock Center (1 mentions) Uas notchrnai, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using dl lacz

Fly Genetics: Lineage Tracing

Cited 1 time

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Fly stocks used in this study: w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP ; UAS-Flp, Act>CD2>Gal4 (Esg FO) (Jiang et al., 2009 (link)), w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP (Esg^ts) (Jiang and Edgar, 2009 (link)), Delta-LacZ (Dl-LacZ, Bloomington 11651), Gbe+Su(H)-lacZ (Su(H)-LacZ, Furriols and Bray, 2001 (link)). Flies were maintained at 25°C, on medium containing agar 0.6% (w/v), malt 6.5% (w/v), semolina 3.2% (w/v), baker’s yeast 1.8% (w/v), nipagin 2.4%, propionic acid 0.7%.

Viitanen A., Gullmets J., Morikka J., Katajisto P., Mattila J, & Hietakangas V. (2021). An image analysis method for regionally defined cellular phenotyping of the Drosophila midgut. Cell Reports Methods, 1(5), 100059.

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Genetic Tools for Drosophila Muscle Development

Cited 1 time

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The sns and hbs double mutant sns^ZF1.4 hbs⁴⁵⁹ was generated for this work by recombining sns^ZF1.4 and hbs⁴⁵⁹, a loss-of-function allele of sns and hbs, respectively, onto the second chromosome. N^fa-g, UAS-Notch RNAi, Dl-lacZ, y w hsFLP, UAS-nlsGFP and Act5C>y+>Gal4 UAS-GFP were provided by the Bloomington Stock Center. rst-Gal4 was obtained from National Institute of Genetics Fly Stock Center (Japan). Other flies used: rst^F6-lacZ[31] (link), spa-Gal4[25] (link), UAS-N-cadherin[12] (link), sns^ZF1.4 and UAS-sns (gift of Susan Abmayr), UAS-N^ICD (gift of Cedric Wesley), UAS-N^ICD-lexA (gift of Toby Lieber), P[w+]36.1 and hbs⁴⁵⁹ (gift of Mary Baylies), UAS-hbs (gift of Helen Sink), GBE-Su(H)_m8-lacZ (N-lacZ) [22] (link), Gal-54[23] (link), UAS-rst (gift of Karl-F. Fischbach), UAS-kirre/duf (gift of Marc Ruiz-Gomez), UAS-Dl (gift of Marek Mlodzik) and hsFLP MKRS (gift of Matthew Freeman).

, & Bao S. (2014). Notch Controls Cell Adhesion in the Drosophila Eye. PLoS Genetics, 10(1), e1004087.

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Drosophila Intestinal Stem Cell Assay

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Fly stocks used in this study: w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP ; UAS-Flp, Act>CD2>Gal4 (Esg FO) (Jiang et al., 2009) , w; Esg-Gal4, Tub-Gal80-ts, UAS-GFP (Esg ts ) (Jiang and Edgar, 2009) , Delta-LacZ (Dl-LacZ, Bloomington 11651), Gbe+Su(H)-lacZ (Su(H)-LacZ, Furriols and Bray, 2001) . Flies were maintained at 25°C, on medium containing agar 0.6% (w/v), malt 6.5% (w/v), semolina 3.2% (w/v), baker's yeast 1.8% (w/v), nipagin 2.4%, propionic acid 0.7%.

Viitanen A., Gullmets J., George J., Katajisto P., Mattila J., & Hietakangas V. (2021). LAM – an image analysis method for regionally defined organ-wide cellular phenotyping of the Drosophila midgut.

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