Immediately following transfer onto PVDF membrane, membrane was rinsed in PBST and stained using Ponceau-S solution (Sigma-Aldrich) for 1 hour at RT. Membrane was washed 3 times in milliQ H2O and imaged on an Epson-Perfection V600 Photo scanner.
Perfection v600 photo scanner
Manufactured by Epson
Sourced in Japan, United States
The Epson Perfection V600 Photo scanner is a high-performance flatbed scanner designed for digitizing photographs, slides, and film negatives. It features a maximum optical resolution of 6400 dpi and supports a wide range of media formats, including 35mm film, medium format film, and 4x5 inch film.
Automatically generated - may contain errors
Lab products found in correlation
Lysis buffer, by Cell Signaling Technology (2 mentions)
Pvdf membrane, by PerkinElmer (2 mentions)
Pathscan intracellular signaling array kit, by Cell Signaling Technology (2 mentions)
Ivis 200 system, by Novus Biologicals (2 mentions)
Axiocam mrc, by Zeiss (2 mentions)
2 3 5 triphenyltetrazolium chloride, by Merck Group (2 mentions)
Novex colloidal blue staining kit, by Thermo Fisher Scientific (2 mentions)
Ponceau s solution, by Merck Group (1 mentions)
Ae100, by Mettler Toledo (1 mentions)
Digital microscope vhx 500f, by Keyence (1 mentions)
Sw55 rotor, by Beckman Coulter (1 mentions)
Gradient master, by BioComp Instruments (1 mentions)
Fraction collector, by Gilson (1 mentions)
Fc 43, by 3M (1 mentions)
Lb broth with agar, by Merck Group (1 mentions)
Isotemp, by Thermo Fisher Scientific (1 mentions)
Fm4 64, by Thermo Fisher Scientific (1 mentions)
Superfrost plus slide, by Thermo Fisher Scientific (1 mentions)
Gr 200 analytical balance, by A&D Company (1 mentions)
Fluostar omega spectrophotometer, by BMG Labtech (1 mentions)
36 protocols using perfection v600 photo scanner
1
Protein Visualization on PVDF Membrane
2
Quantitative Immunoblotting and Signaling Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunoreactivity studies, tissue samples were diluted to 100 mg/mL in lysis buffer (#7018s, Cell Signaling Technology) and subsequently homogenized, sonicated, and spun at 3.5K RPM for 1 min. Lysate samples were then screened against an array of target-specific capture antibodies using PathScan Intracellular Signaling Array Kits (#7323, Cell Signaling Technology) in accordance with the manufacturer’s instructions and developed using HyBlot CL autoradiography film (#e3012, Denville Scientific Inc.). Processed films were digitized using an Epson Perfection V600 Photo scanner and the relative pixel intensities for each blot quantified using the image processing software ImageJ. Complementary western blot studies were performed by loading 20-40 µg of protein into 7.5-12% SDS PAGE gels, transferred to PVDF membranes (PerkinElmer), and resolved by electrophoresis. Membranes were blocked overnight at 4°C in trisbuffered saline 0.1% Tween-20 (TBST) containing 5% w/v nonfat dry milk powder and subsequently incubated with antibodies to p-ERK 1/2 Thr202/Tyr204 (Cell Signaling, 4370), pS6 (Cell Signaling, #4858) LC3B, (anti LC3B, Cell signaling 2775), or b-tubulin (Novus Biologicals, NB600-936); Membrane chemiluminescence was imaged on a Xenogen IVIS 200 system.
3
Imaging and Statistical Analysis of Plant Root and Rosette
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the end of each experiment, Petri dishes were scanned (Epson Perfection V600 Photo Scanner), and the images were analyzed using image analysis. The primary root length and rosette areas were calculated using the Image J software (Poupin et al., 2016 (link)). The root area and the non-green rosette area were calculated using the Adobe Photoshop software as described in Pinedo et al. (2015) (link). When the experiments considered two factors (bacteria and nutrients), two-way ANOVA was used, followed by a multiple comparisons test. When data was not parametric, Kruskal-Wallis or Mann-Whitney tests were performed to compare subgroups of each data (i.e., comparing bacterial treatments and the non-inoculated group under the same nutritional treatment). The replicate number is indicated in each experiment.
4
Fungal Growth Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Overnight cultures of H99 and the pCTR4-2-FKS1 strain were diluted to an optical density at 600 nm (OD600) of 1, and then 10-fold serial dilutions were prepared in PBS. A 5-μL aliquot of each dilution was spotted on the indicated medium, then incubated for 48 to 72 h at 30°C or 37°C. Plates were imaged using an Epson Perfection V600 Photo Scanner. Contrast was adjusted equally across all images for the best visualization of colonies.
5
Stem Length, Pod, and Seed Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At maturity, stem length was measured as the distance between the node of the first pod to the top of the stem. Pods and seeds were collected from the main stems of each plant and dried at 43 °C for five days. Only the main stem was evaluated in this study. Pods and seeds were scanned with an Epson Perfection V600 Photo Scanner at 600dpi. For image scanning, pod halves, lying flat on the surface, and seeds were spread uniformly so that no individual pods or seeds were in contact. Images for pods and seeds were taken separately and analyzed for morphological traits using the high-throughput seed measurement software SmartGrain [27 (link)]. Within the software, a common scale was defined, and the visible surface area of pods and seeds was extracted and used as phenotypic data. All collected seeds from each plant were weighed using a balance (AE100, Mettler).
6
Detailed Seed Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 20 DAP (Days After Pollination) siliques were harvested once they had turned brown before dropping seeds and stored in paper envelopes before seed size analysis. Dried silique material and aborted seeds were removed using forceps, seeds were spread onto an EPSON Perfection V600 Photo Scanner and evenly separated using a painting brush to ensure further single-seed measurements. Images were taken at a resolution of 900 dpi in a black and white format. ImageJ was used to measure seed area (Abramoff et al., 2004 ), with a range of 0.08–0.3 mm2 for diploid and paternal-excess triploid seeds and 0.03–0.2 mm2 for maternal-excess triploid seeds to exclude any non-seed material.
7
Fungal Growth Behavior Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To define growth behavior and sexual development, S. macrospora strains were grown on solid SWG medium over 10 d and documented with a VHX-500F Digital Microscope (Keyence, Osaka, Japan). For growth-rate determination, six replicates each of S. macrospora wt strain, Sm::Smarp1-TagRFP-T and Sm::Smarp1-mNG, were grown in 30-cm race tubes filled with solid SWG medium over 7 d under continuous light at 27 °C. After 3 d, the growth front was marked every day at the same time to determine the growth velocity in cm/day.
For the determination of the C. graminicola growth rate, defined mycelial plugs of CgM2, CgM2::Arp1-TagRFP-T, and CgM2::SmArp1-mNG from pre-cultures were transferred to fresh CM plates without selection. After 3 d, the growth area was recorded using an Epson Perfection V600 Photo scanner in four subsequent days. The optical evaluation of the growth area was done using the measuring tool of Fiji [71 (link)] on scaled images. The growth rate of four replicates was determined by the difference of the growth area of two subsequent days in cm2.
For the determination of the C. graminicola growth rate, defined mycelial plugs of CgM2, CgM2::Arp1-TagRFP-T, and CgM2::SmArp1-mNG from pre-cultures were transferred to fresh CM plates without selection. After 3 d, the growth area was recorded using an Epson Perfection V600 Photo scanner in four subsequent days. The optical evaluation of the growth area was done using the measuring tool of Fiji [71 (link)] on scaled images. The growth rate of four replicates was determined by the difference of the growth area of two subsequent days in cm2.
8
Fractionation of DNAJB6 Oligomers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The polydispersity of the DNAJB6 oligomers prevented single-particle reconstruction on the samples earlier analyzed by negative stain EM26 (link) hence in order to improve the specimen for EM studies we here fractionated the DNAJB6 oligomers using the GraFix procedure to reduce the sample complexity by a gradient fixation protocol72 (link),73 , in which the sample is subjected to weak intramolecular chemical crosslinking during density gradient ultracentrifugation. A 4 ml linear 5–30% (w/w) sucrose and 0–0.2% (w/w) glutaraldehyde gradient in buffer (20 mM NaPO4, 150 mM NaCl, pH 8.0) was prepared using a gradient master (Biocomp, Canada) mixer. In total, 200 µl of DNAJB6 solution (0.4 mg/ml) was added on top of the gradient and run for 16 h at 4 °C at a speed of 30,000 rpm in a Beckman SW 55 rotor. Fractions of approx. 250 µl were collected from the bottom of the tube using a fraction collector (Gilson, USA) and analyzed by blue native-PAGE on precast 4–16% Bis-Tris Gels (Life Technologies, Sweden) and scanned with an Epson Perfection V600 photo scanner.
9
Wheat Leaf Rust Inoculation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
P. triticina isolates B9414, 93012, 94015, 95012, 96007 and 96237 are avirulent on Lr9 (refs. 52 ,53 (link)). Isolate MNPSD is virulent on Lr9 (ref. 54 (link)). Isolates were propagated on seedlings of the susceptible wheat cultivar Thatcher. Freshly collected urediniospores were used for inoculation experiments. Inoculations were performed using a high-pressure air sprayer or a settling tower. For the spray inoculation, urediniospores were suspended in FC-43 oil (3M Fluorinert FC-43) and sprayed onto plants using a glass sprayer connected to a high-pressure air pipe. For the settling tower inoculation, plants were grown for 12 d. Then, the second leaf was removed and the first leaf was pinned to the soil with the adaxial side facing upward. A mixture of 10 mg urediniospores and 300 mg lycopodium powder (Sigma-Aldrich, 19108) was blown over the plants in a settling tower55 (link). Inoculated plants were placed in an inoculation box equipped with a humidifier overnight and transferred to a walk-in Conviron growth room (16 h day/8 h night, 18 °C/16 °C). Symptoms were evaluated 12 d after inoculation. Infected leaves were scanned using an Epson Perfection V600 Photo scanner.
10
Doxycycline Impacts Wound Bacterial Burden
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!