BALB/c female mice (n = 6 per group, 7-8 weeks old) and DBA/2 female mice (7 to 8 weeks old) were purchased from HFK Bioscience Co. Ltd. (Beijing, China). Animal care and use were in accordance with the institutional guidelines. All animal experiments were approved by the Institutional Animal Care and Use Committee of the National Institute of State Scientific and Technological Commission.
Female balb c mice
Manufactured by Beijing Huafukang Biotechnology
Sourced in China
Female BALB/c mice are a commonly used laboratory mouse strain. They are characterized by their white coat color and susceptibility to certain types of cancer. These mice are widely used in various fields of biomedical research, including immunology, oncology, and genetics.
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Lab products found in correlation
Dba 2 female mice, by Beijing Huafukang Biotechnology (2 mentions)
H11 k18 hace 2 female mice, by Beijing Huafukang Biotechnology (2 mentions)
Female balb c nude mice, by Beijing Huafukang Biotechnology (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
C57bl 6 mice, by Beijing Huafukang Biotechnology (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
ε caprolactone, by Merck Group (1 mentions)
Acid tetrachloroaurate 3 trihydrate haucl4 3h2o, by Sinopharm (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Imject alum adjuvant, by Thermo Fisher Scientific (1 mentions)
Ova grade 5, by Merck Group (1 mentions)
Ct 26 cell line, by National Collection of Authenticated Cell Cultures (1 mentions)
Mhv 3, by American Type Culture Collection (1 mentions)
Be0061, by BioXCell (1 mentions)
Bp0101, by BioXCell (1 mentions)
2 ome 5 chol, by RiboBio (1 mentions)
31 protocols using female balb c mice
1
Evaluating Mouse Strain Responses
2
Breast Cancer Cell Line 4T1 in BALB/c Mice
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The mouse breast cancer (4T1) cell line was purchased from Peking Union Medical College Cell Center and cultured in RPMI-1640 medium containing 10% fetal bovine serum, 100 units of penicillin, and streptomycin under the condition of 37 °C and 5% CO2.
BALB/c female mice (20 ± 2 g) were purchased from Beijing HFK Bioscience Co., Ltd. (Beijing, China). The mice were raised in an SPF-level laboratory animal room, kept on a 12 h light–dark cycle, and provided a standard diet of food and water ad libitum. All the experimental procedures complied with the Guidelines and Policies for Ethical and Regulatory for Animal Experiments and approved by the Animal Ethics Committee of Peking Union Medical College (Beijing, China).
BALB/c female mice (20 ± 2 g) were purchased from Beijing HFK Bioscience Co., Ltd. (Beijing, China). The mice were raised in an SPF-level laboratory animal room, kept on a 12 h light–dark cycle, and provided a standard diet of food and water ad libitum. All the experimental procedures complied with the Guidelines and Policies for Ethical and Regulatory for Animal Experiments and approved by the Animal Ethics Committee of Peking Union Medical College (Beijing, China).
3
Comparative Study of BALB/c and DBA/2 Mice
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BALB/c female mice (n = 6 per group, 7 to 8 weeks old) and DBA/2 female mice (7 to 8 weeks old) were purchased from HFK Bioscience Co. Ltd. (Beijing, China). Animal care and use were conducted in accordance with institutional guidelines, and all animal experiments were approved by the Institutional Animal Care and Use Committee of the National Institute of State Scientific and Technological Commission.
4
Mouse Model for SARS-CoV-2 Studies
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Specific pathogen-free (SPF) BALB/c female mice (15 to 19 g) and H11-K18-hACE-2 female mice (5 weeks) were purchased from Beijing HFK Bioscience Co., Ltd., and Gempharmatech Co., Ltd., and were kept under standard pathogen-free conditions at Yikang (Beijing) Pharmaceutical Technology Co., Ltd., and the animal experimental center in the BSL-3 at the Chinese National Institute for Viral Disease Control and Prevention, China CDC, respectively. All mouse experiments were approved by the Animal Care Welfare Committee of Yikang (Beijing) Pharmaceutical Technology Co., Ltd. (Animal Experimental Ethical Inspection Form YK-2021-005), National Institute for Viral Disease Control and Prevention (IVDC), China CDC (Animal Experimental Ethical Inspection Form 20210830068 and BSL-3 Animal Experimental Ethical Inspection Form 121051018) and were conducted according to the Guide for Care and Use of Laboratory Animals.
5
Mouse Model for SARS-CoV-2 Studies
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Specific pathogen-free (SPF) BALB/c female mice (15 to 19 g) and H11-K18-hACE-2 female mice (5 weeks) were purchased from Beijing HFK Bioscience Co., Ltd., and Gempharmatech Co., Ltd., and were kept under standard pathogen-free conditions at Yikang (Beijing) Pharmaceutical Technology Co., Ltd., and the animal experimental center in the BSL-3 at the Chinese National Institute for Viral Disease Control and Prevention, China CDC, respectively. All mouse experiments were approved by the Animal Care Welfare Committee of Yikang (Beijing) Pharmaceutical Technology Co., Ltd. (Animal Experimental Ethical Inspection Form YK-2021-005), National Institute for Viral Disease Control and Prevention (IVDC), China CDC (Animal Experimental Ethical Inspection Form 20210830068 and BSL-3 Animal Experimental Ethical Inspection Form 121051018) and were conducted according to the Guide for Care and Use of Laboratory Animals.
6
4T1 Tumor Induction in Balb/c Mice
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Animals received care under the instruction of the Guidance Suggestions for the Care and Use of Laboratory Animals. Balb/c female mice (6 weeks) were purchased (Beijing HuaFuKang Bioscience Co. Ltd, China). To obtain tumor-bearing mice, hairs on the upper limb were removed. Then, 1 × 107 4T1 cells were subcutaneously injected into the right upper limb of each mouse. The tumor bearing mice was used for further experiments when the tumor volume reached 60–250 mm3.
7
Nude Mice Xenograft Model for CCDC7-shRNA Therapy
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Four- to six-week-old nude BALB/c female mice were obtained from HFK Bioscience (Beijing, China) and maintained at the Sichuan University, Chengdu. Animals were inoculated subcutaneously (s.c.) with of a total of 100 μL containing 1 × 107 HeLa cells into the right shoulder. The interval between the first and the final inoculation into the mice was less than 1 h.
When the mean diameter of the tumors reached 5 mm (5 days after inoculation), the mice were separated at random into three groups (n = 8 per group): the NS group, receiving 100 μL saline; the control-shRNA group, receiving 10 μg of the HK plasmid and 50 μg liposome complexes (final volume = 100 μL); and the CCDC7-shRNA group, receiving 10 μg of the shRNA plasmid and 50 μg liposome complexes (final volume = 100 μL). Nude mice were treated once daily for 10 days via injections around the tumor.
Tumor size was monitored by measuring the largest diameter and the diameter perpendicular to the largest diameter using a caliper once every 3 days. Tumor volume was calculated according to the following formula: V = length × width2 × 0.52.19 (link) All of the data are represented as means ± standard errors of the means (SEMs). After 25 days of treatment, the mice were sacrificed, and tumors were isolated and fixed in 4% neutral formalin for histological analysis.
When the mean diameter of the tumors reached 5 mm (5 days after inoculation), the mice were separated at random into three groups (n = 8 per group): the NS group, receiving 100 μL saline; the control-shRNA group, receiving 10 μg of the HK plasmid and 50 μg liposome complexes (final volume = 100 μL); and the CCDC7-shRNA group, receiving 10 μg of the shRNA plasmid and 50 μg liposome complexes (final volume = 100 μL). Nude mice were treated once daily for 10 days via injections around the tumor.
Tumor size was monitored by measuring the largest diameter and the diameter perpendicular to the largest diameter using a caliper once every 3 days. Tumor volume was calculated according to the following formula: V = length × width2 × 0.52.19 (link) All of the data are represented as means ± standard errors of the means (SEMs). After 25 days of treatment, the mice were sacrificed, and tumors were isolated and fixed in 4% neutral formalin for histological analysis.
8
Xenograft Tumor Models in BALB/c Mice
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Female BALB/c nude mice (4 weeks old, 15–17 g) and female BALB/c mice (6 weeks old, 22–25 g) (Beijing HFK Bioscience Co., Ltd., Beijing, China) were kept in a specific pathogen-free barrier system room with constant ambient temperature under a 12 h/12 h light/dark cycle. All animal experiments were performed according to the guidelines approved by the Institutional Animal Care and Use Committee of Tongji Medical College, Huazhong University of Science and Technology. For xenograft tumor models, BALB/c nude mice were subcutaneously injected in the right upper front leg with either MDA-MB-231 cells (1 × 107 in 150 µL PBS) or MCF-7 cells (5 × 106 in 150 µL PBS). Tumors were grown 14 to 21 days until the maximal dimension reached 0.8∼1.0 cm for PET imaging.
9
Anti-IL-17A Antibody Inhibits CXCL1 in Mice
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Example 6
Female BALB/c mice, 6 to 8 weeks old, purchased from Beijing Huafukang Biotechnology Co., Ltd., were used as experimental animals. One week after the mice were acclimated to the environment, the mice were randomly divided into groups, 6 per group. Each group was given anti-human IL-17A chimeric monoclonal antibody or control monoclonal antibody Secukinumab, at three doses of 0.7 nmol/kg, 7 nmol/kg or 70 nmol/kg, intravenous injection, single administration. One hour after the administration, human IL-17A was injected subcutaneously, 10 μg per mouse. Two hours later, retro-orbital blood sample was collected without anticoagulation, and the blood sample was allowed to stand at room temperature for 30 minutes to 1 hour. After the coagulation of blood, the sample was centrifuged at 3000 rpm for 10 minutes to obtain a serum sample. The concentration of mouse CXCL1 (C-X-C Motif Chemokine Ligand, also known as KC) in the serum was detected according to the instructions using a mouse CXCL1 ELISA kit (RayBiotech, Catalog Number ELM-KC).
The result is shown in
10
Immunomodulatory Effects of Polysaccharides in Mice
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Female Balb/c mice (6–8 weeks old) were obtained from the Beijing HFK Bioscience Co. Ltd. (HFK). The animal studies were approved by the Animal Experimentation Ethics Committee of College of Life Science and Technology, Huazhong University of Science and Technology. All procedures were carried out in strict compliance with the animal care guidelines of the Science and Technology Department of Hubei Province.
The Balb/c mice were randomly divided into 6 groups—control group, model group, and polysaccharides groups (lentinan, tremellan, pachymaran, or mixture of three). The mice were given polysaccharides (200 mg/kg) for 30 days in the groups pretreated with polysaccharides, while the mice in the control and model groups were served with the same volume of PBS. From days 21 to 23, cyclophosphamide (80 mg/kg) was injected intraperitoneally to induce the immunosuppression in the model group and polysaccharides groups (Scheme 1 ). The mice of each group were weighed every day after treatment with cyclophosphamide. Sera, thymus, and spleens of mice were collected at the end of experiments.
The Balb/c mice were randomly divided into 6 groups—control group, model group, and polysaccharides groups (lentinan, tremellan, pachymaran, or mixture of three). The mice were given polysaccharides (200 mg/kg) for 30 days in the groups pretreated with polysaccharides, while the mice in the control and model groups were served with the same volume of PBS. From days 21 to 23, cyclophosphamide (80 mg/kg) was injected intraperitoneally to induce the immunosuppression in the model group and polysaccharides groups (
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