NT2D1 were cultured as described above for 24 h. Samples then were fixed in 2.5% glutaraldehyde in cacodylate buffer (0.1 M pH 7.3) overnight, and post-fixed with 1% osmium tetroxide in cacodylate buffer (1 M). Then, samples were dehydrated with increasing ethanol percentage (30–90% in water for 5 min, twice at 100% for 15 min), dried in a critical point dryer (EMITECH K850), sputter coated with platinum–palladium (Denton Vacuum DESKV), and observed with a Supra 40 FE SEM (Zeiss).
Supra 40 fesem
Manufactured by Zeiss
Sourced in Germany
The Supra 40 FESEM is a high-resolution field emission scanning electron microscope (FESEM) manufactured by Zeiss. It is designed to provide detailed images and analysis of a wide range of materials and samples at the nanoscale level. The Supra 40 FESEM utilizes a field emission gun to generate a focused electron beam, enabling high-resolution imaging with minimal sample preparation.
Automatically generated - may contain errors
Lab products found in correlation
Cm10 tem, by Zeiss (2 mentions)
Tga 50 thermogravimetric analyzer, by Shimadzu (1 mentions)
Inova 400 mhz spectrometer, by Agilent Technologies (1 mentions)
D max 2550 x ray diffractometer, by Rigaku (1 mentions)
Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
X pert mpd diffractometer, by Philips (1 mentions)
H7600 tem, by Hitachi (1 mentions)
Desk 5, by Denton (1 mentions)
Microscope glass 12 mm, by Thermo Fisher Scientific (1 mentions)
Cm12 electron microscope, by Philips (1 mentions)
14 protocols using supra 40 fesem
1
SEM Imaging of NT2D1 Cell Morphology
2
Electron Microscopy Sample Preparation
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Samples were fixed in Glutaraldehyde 2.5% in cacodylate buffer 0.1 M pH 7.3 ON and then postfixed with 1% osmium tetroxide in cacodylate buffer 1 M, dehydrated with increasing ethanol percentage (30–90% in water for 5 min, twice 100% for 15 min), treated in Critical Point Dryer (EMITECH K850), sputter coated with platinum-palladium (Denton Vacuum DESKV), and observed with Supra 40 FESEM (Zeiss).
3
Physicochemical Characterization of PAO
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The physicochemical properties of PAO were characterized by Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, scanning electron microscopy (SEM), powder X-ray diffraction (XRD), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and solid-state nuclear magnetic resonance (NMR) spectroscopy in detail. Except for the TGA measurement, all characterizations were performed at room temperature. FT-IR spectroscopy measurement was mounted on a Thermofisher Nicolet IS 50 FT-IR spectrometer. Raman spectroscopy analysis was performed on a LabRam HR Raman spectrometer. SEM images were obtained with a ZEISS SUPRA 40 FE-SEM. Powder XRD patterns were collected on a RigaKu D/max 2550 X-ray Diffractometer with CuKα radiation (λ = 0.15406 nm). TGA curve measurement was examined on a Shimadzu TGA-50 thermogravimetric analyzer from room temperature to 800 °C at a heating rate of 10 °C min−1 with a nitrogen flow rate of 50 mL min−1. XPS spectroscopy was performed on an ESCALab220i-XL surface microanalysis system (VG Scientific) equipped with an Al Kα (hλ = 1486.6 eV) source at a chamber pressure of 3 × 10−9 mbar. The surface charging effect was corrected with C 1s peak at 284.4 eV as a reference. Solid-state 1H and 13C NMR spectra were acquired on a Varian Inova 400 MHz spectrometer.
4
Structural and Compositional Analysis of Nanoparticles
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Sizes and morphologies of ASNs and MSNs were investigated by field emission scanning electron microscopy (FESEM) using a Zeiss Supra 40 FESEM unit at an accelerating voltage of 5 to 10 kV and by transmission electron microscopy (TEM) using a Hitachi H-7600 TEM operating at an accelerating voltage of 80 kV. High-resolution TEM (HRTEM) and scanning TEM (STEM) analyses were performed on an FEI TALOS F200X TEM operating at an accelerating voltage of 200 kV. The chemical compositions of ASNs and MSNs were analyzed simultaneously using an energy dispersive X-ray spectroscopy (EDS) detector attached to Zeiss Supra 40 FESEM. X-ray powder diffraction (XRD) patterns were produced using a Philips X’Pert-MPD Diffractometer equipped with a monochromatic X-ray of Cu Kα1 radiation (λ = 0.15405 nm) at 1.6 kW (40 kV, 30 mA).
5
S. epidermidis Colonization on Coated Threads
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Samples of threads gauge 2 (Coated, Braided Lactomer 9-1) colonized by S. epidermidis, were fixed in paraformaldehyde (4%) in phosphate saline buffer over night and then dehydrated with increasing ethanol percentage (30–90% in water for 5 min, twice 100% for 15 min). The samples were then treated in a Critical Point Dryer (EMITECH K850, Quorum technologies Ltd., Laughton, East Sussex, UK) sputter coated with platinum-palladium (Denton Vacuum DESKV, Denton Vacuum, Moorestown, NJ, USA) and observed with Supra 40 FE-SEM (Zeiss, Germany).
6
Glutaraldehyde-fixed Sample Preparation
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Glutaraldehyde-fixed samples were rinsed with a cacodylate buffer and then dehydrated with an increasing ethanol percentage (30–90% in water for 5 min, twice 100% for 15 min), treated in a Critical Point Dryer (EMITECH K850), sputter coated with platinum-palladium (Denton Vacuum DESKV), and observed with Supra 40 FESEM (Zeiss).
7
Ultrastructural Mapping of C. elegans Neuron Connectivity
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Four samples were fixed by chemical fixation or high pressure freezing and freeze substitution as previously described 49 (link). Ultrathin sections were cut using a RMC Powertome XL, collected onto grids, and imaged using either a Philips CM10 TEM or Zeiss Supra 40 FE-SEM. Sections were elastically aligned 50 and volumetrically reconstructed using TrakEM2 51 (link) . The MCM cell bodies were identified in the EM sections based on position and morphology and in comparison to similar hermaphrodite sections. This identity was further confirmed by the identification of a posterior projection, as no other cell with its soma in the same region is known to project posteriorly. This was followed by serial tracing of the projections to establish their morphology and connectivity. Synaptic connectivity and skeleton diagrams were determined using Elegance 52 (link) . Circuit diagrams of connectivity were generated using Cytoscape 53 (link). We estimated the anatomical strength of synaptic connectivity between two neurons by summing the number of serial sections where we observed the ultrastructural morphology presynaptic components using the same criteria as 14
8
FESEM Imaging of α Rifaximin Powder
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Images of the powder of four batches of α rifaximin were recorded with a SUPRA™ 40 FESEM (Zeiss, Germany) using a voltage of 1 kV.
Each sample was placed on a small piece of double-sided conductive tape that had been previously applied on a metallic sample holder. A weak nitrogen flow was used to remove the particles in excess. Images were recorded at magnification ranging from 300× to 5000×.
Each sample was placed on a small piece of double-sided conductive tape that had been previously applied on a metallic sample holder. A weak nitrogen flow was used to remove the particles in excess. Images were recorded at magnification ranging from 300× to 5000×.
9
Ultrastructural Mapping of C. elegans Neuron Connectivity
10
Morphology and Composition Analysis of PDDA/CNT HYAM
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A cross-sectional section of the yarn was prepared using a Focused Ion Beam (FEI Nova 200 NanoLab DualBeam FIB/SEM). This cross-section of the coiled PDDA/CNT HYAM was imaged using a scanning electron microscope (Zeiss SUPRA 40 FE-SEM) and the elemental composition was analyzed using an EDAX Lithium detector. The morphology of the PDDA/CNT HYAM was observed by FE-SEM using a Low Vacuum & Bio Application Technology instrument (Quanta 250 FEG).
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