To morphologically characterize the isolated membrane fraction, specimens were diluted 1:1,000 in PBS, and absorbed onto freshly cleaved mica sheets for 20 min. To provide a surface coated with formulations to a suitable density, the mica sheets were rinsed three times with deionized water and then dried with filter paper before detection. Surface morphology was examined under an atomic force microscope (Asylum Research MFP-3D-Bio; Digital Instruments, Santa Barbara, CA, USA) as described by Mu et al. (2014) (link). The particle size distribution of EPDELNs was evaluated using a Light Scattering System (Brookhaven BI-200SM) as previously described (Mu et al., 2014 (link)). Measurements were made in PBS at pH 7.0 at 25 °C after appropriate dilution of each EPDELN sample.
Mfp 3d bio
Manufactured by Digital Instruments
Sourced in United States
The MFP-3D-Bio is a high-performance atomic force microscope (AFM) designed for biological and life science applications. It provides precise topographical imaging and force measurements of delicate biological samples, such as cells, proteins, and DNA. The MFP-3D-Bio delivers high-resolution data with advanced imaging modes and a user-friendly interface, enabling researchers to gain valuable insights into the nanoscale structure and properties of their samples.
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Lab products found in correlation
2 protocols using mfp 3d bio
1
Membrane Fraction Characterization by AFM
2
Isolation and Characterization of Umbilical Cord Blood Exosomes
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An anticoagulant solution of 15% EDTA was prepared, and 2 drops were combined in a 5 ml syringe before collecting umbilical cord blood. At the time of birth, the piglets were placed under a heat lamp, UA and UV blood samples were collected from the umbilical cord. Samples were inverted several times to ensure inhibition of clotting by the EDTA solution. The blood was then transferred to 2 ml centrifuge tubes (1 ml per tube). Exosomes in the UCB were isolated as previously described by Théry et al. 21 . The umbilical cord blood exosomes were prepared as previously described by Palanisamy et al. [22]for atomic force microscopy (AFM) analysis. The exosome suspensions were diluted 1:500 in deionized water, and were then adsorbed to freshly cleaved mica sheets for 10 min. Excess exosome suspension was carefully removed with absorbent filter paper, and the mica was further dried before analysis. Surface morphology was examined under an atomic force microscope (Asylum Research MFP-3D-Bio, Digital Instruments Inc., Santa Barbara, CA).
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