An ELISA quantitative kit for the detection of human IgG and IgM (Bethyl Laboratories, Montgomery, TX) was used to measure the concentrations of total IgG and IgM from culture supernatants and plasma. The ELISA kits of human fatty acid binding protein 2 (FABP2, Cat#: DY3078) and CXCL13 (Cat#: DCX130) were purchased from R&D System Inc (Minneapolis, MN, USA), and were used measure their concentration in the plasma from LC and HC subjects.
Fabp2
Manufactured by R&D Systems
Sourced in United States
FABP2 is a laboratory product manufactured by R&D Systems. It is a Fatty Acid Binding Protein 2 that can be used for research purposes. The core function of FABP2 is to facilitate the intracellular transport and metabolism of long-chain fatty acids.
Automatically generated - may contain errors
Lab products found in correlation
Cxcl13, by R&D Systems (1 mentions)
Lipopolysaccharide lps binding protein lbp, by Hycult Biotech (1 mentions)
Salmonella typhimurium, by InvivoGen (1 mentions)
Endocab, by Hycult Biotech (1 mentions)
Soluble cd14 scd14, by R&D Systems (1 mentions)
Pecam 1, by R&D Systems (1 mentions)
Erbb2, by R&D Systems (1 mentions)
Synergy 4 microplate reader, by Agilent Technologies (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Calprotectin, by Immundiagnostik (1 mentions)
Zonulin, by Immundiagnostik (1 mentions)
4 protocols using fabp2
1
Quantifying Immunoglobulins and Biomarkers
2
Serological Markers of Celiac Disease
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Established serological markers of coeliac disease, including IgA antibody to TG2 and IgG and IgA antibodies to deamidated gliadin, were measured as previously described.24 (link)
25 (link)
Serum IgG, IgA and IgM antibodies to native gliadin were measured separately by ELISA as previously described,24 (link)
26 (link) with the following modification: the secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-human IgG (GE Healthcare), IgA (MP Biomedicals) or IgM (MP Biomedicals). Serum IgG, IgA and IgM antibodies to bacterial flagellin were measured separately using a similar protocol for detecting antibodies to gliadin, with the following modification: plates were coated with a 2 μg/mL solution of highly purified flagellin from Salmonella typhimurium (InvivoGen).
Levels of serum IgG, IgA and IgM endotoxin-core antibodies (EndoCAb) (Hycult Biotech), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems) and fatty acid-binding protein 2 (FABP2) (R&D Systems) were determined by ELISA, according to the manufacturers' protocols.
25 (link)
Serum IgG, IgA and IgM antibodies to native gliadin were measured separately by ELISA as previously described,24 (link)
26 (link) with the following modification: the secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-human IgG (GE Healthcare), IgA (MP Biomedicals) or IgM (MP Biomedicals). Serum IgG, IgA and IgM antibodies to bacterial flagellin were measured separately using a similar protocol for detecting antibodies to gliadin, with the following modification: plates were coated with a 2 μg/mL solution of highly purified flagellin from Salmonella typhimurium (InvivoGen).
Levels of serum IgG, IgA and IgM endotoxin-core antibodies (EndoCAb) (Hycult Biotech), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems) and fatty acid-binding protein 2 (FABP2) (R&D Systems) were determined by ELISA, according to the manufacturers' protocols.
3
Biomarker Quantification by ELISA
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Human-specific ELISA kits were employed to measure the levels of IGFBP-1 (not the complexed form), receptor tyrosine-protein kinase erbB-2 (ErbB2), fatty acid-binding protein 2 (FABP2) and platelet endothelial cell adhesion molecule (PECAM-1) (all from R&D Systems Inc., Abingdon, UK), according the manufacturer’s instructions. The test detection limits were 6.36, 4.86, 3.63, and 21 pg/mL, respectively. The photometric measurements were performed using a Synergy 4 microplate reader (BioTek Instruments Inc., Winooski, VT, USA).
4
Biomarkers of Kidney Transplant Function
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Biochemical parameters (serum creatinine and C-reactive protein (CRP) levels, alanine aminotransferase (ALT), and gammaglutamyltranspeptidase (GGT) activity) were measured using standard laboratory methods. Kidney graft function was assessed using an estimated glomerular filtration rate (eGFR) calculated according to the Modification of Diet in Renal Disease formula.
Plasma concentrations of zonulin and calprotectin (Immundiagnostik AG, Bensheim, Germany) were assessed using commercially available ELISA kits, with the intra-assay and inter-assay coefficients of variability being <5 and <8.5% and <3.3 and <9.0%, respectively. Plasma levels of IL-6, CD-14 (R&D Systems, Minnesota, MN, USA), and bacterial lipopolysaccharides (Uscn Life Sciences Inc., Wuhan, China) were assessed by ELISA, with the intra-assay and inter-assay coefficients of variability being <7.2 and <7.8%, <6.4 and <7.4%, and <10 and <12%, respectively. Plasma FABP-2 (R&D systems, Inc., Minneapolis, MN, USA) and LBP levels (CloudClone Corp, Katy, TX, USA) were measured by ELISA kit with the intra-assay and inter-assay coefficients of variability being <4.1 and 11.1% and <10 and <12%, respectively.
Plasma concentrations of zonulin and calprotectin (Immundiagnostik AG, Bensheim, Germany) were assessed using commercially available ELISA kits, with the intra-assay and inter-assay coefficients of variability being <5 and <8.5% and <3.3 and <9.0%, respectively. Plasma levels of IL-6, CD-14 (R&D Systems, Minnesota, MN, USA), and bacterial lipopolysaccharides (Uscn Life Sciences Inc., Wuhan, China) were assessed by ELISA, with the intra-assay and inter-assay coefficients of variability being <7.2 and <7.8%, <6.4 and <7.4%, and <10 and <12%, respectively. Plasma FABP-2 (R&D systems, Inc., Minneapolis, MN, USA) and LBP levels (CloudClone Corp, Katy, TX, USA) were measured by ELISA kit with the intra-assay and inter-assay coefficients of variability being <4.1 and 11.1% and <10 and <12%, respectively.
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