Phloretin

Manufactured by Yuanye Bio-Technology

Sourced in China

Phloretin is a laboratory reagent used in biochemical research. It functions as a selective inhibitor of glucose transporters.

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Lab products found in correlation

4 protocols using phloretin

Antioxidant and Cytotoxicity Evaluation

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Phloretin (Phl, purity ≥ 98%), isoniazid (Inz, purity = 98%), 2-picolinic acid (99%), theophylline (99%), 4-aminobenzamide (98%), proline (99%), nicotinic acid (99%) and 4-picolinic acid (99%) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Phloretin (HPLC ≥ 98%) and maleic (99%) acid were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was purchased from Sigma Life Science (St. Louis, MO, USA). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Methanol was obtained from Guangzhou Reagent Co., Ltd. (Guangzhou, China). The A549 cell line and HepG2 cell line were purchased from the Institute of Biochemistry and Cell Biology Sciences, Chinese Academy of Sciences (Shanghai, China).

Lu Z., Chen H., Mo J., Yuan X., Wang D., Zheng X, & Zhu W. (2023). Cocrystal of phloretin with isoniazid: preparation, characterization, and evaluation. RSC Advances, 13(16), 10914-10922.

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Preparation of Polyphenol Standard Solutions

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Chlorogenic acid (CAS: 327-97-9), neoChlorogenic acid (CAS: 906-33-2), trans-cinnamic acid (CAS: 140-10-3), ferulic acid (CAS: 1135-24-6), p-coumaric acid (CAS: 501-98-4), caffeic acid (CAS: 331-39-5), p-hydroxybenzoic acid (CAS: 99-96-7), epicatechin (CAS: 490-46-0), (+)-catechin (CAS: 154-23-4), (−)-epigallocatechin (CAS: 970-74–1), (−)-gallocatechin (CAS: 3371-27-5), phloretin (CAS: 60-82-2) and phlorizin (CAS: 60-81-1) were purchased from Shanghai Yuanye Bio-technology Co., Ltd (Shanghai, China). All phenolic chemicals were analytical standards, with HPLC purity ≥ 98%.
Stock solutions of polyphenols were obtained by dissolving each polyphenol in methanol containing 5% dimethyl sulfoxide (DMSO). Subsequently, each polyphenol was diluted with sterilized NB medium to the appropriate concentration. All solutions were prepared fresh and filter-sterilized (pore size, 0.22 μm) prior to use.

Zhang Y., Wei J., Qiu Y., Niu C., Song Z., Yuan Y, & Yue T. (2019). Structure-Dependent Inhibition of Stenotrophomonas maltophilia by Polyphenol and Its Impact on Cell Membrane. Frontiers in Microbiology, 10, 2646.

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Quantifying Plant Flavonoid Levels

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The total flavonoid levels of leaf samples were then extracted and measured by the NaNO₂-Al(NO₃)₃-NaOH system according to prior research [43 (link)], with rutin (Yuanye, Weifang, China) as reference. Three representative DHCs (phlorizin, trilobatin, and phloretin) were identified and quantified through HPLC analysis based on the study of Wei et al. (2020) [44 (link)], with minor modification. In brief, chromatographic analysis was performed using the Shimadzu LC-20 instrument with an SPD-M20A diode array detector, on an InertSustain C18 analytical column (250 mm × 4.6 mm, 5 µm) at 40 °C. Before measurement, the DHC extracts were dissolved in 30 mL of 70% ethanol using an ultrasonic method, and the supernatant was filtered through a 0.22 μm membrane filter. The mobile phase was gradient-eluted with acetonitrile (solvent A) and 0.04% (v/v) formic acid (solvent B). The flow rate was set at 0.8 mL min⁻¹, and the detection wavelength was 285 nm. Phlorizin, trilobatin, and phloretin standards (Yuanye, China) were used as the standard calibration curves.

Zhang W., Sun Y., Wang H., Xu M., He C., Wang C., Yu Y., Zhang Z, & Su L. (2024). Exogenous Melatonin Enhances Dihydrochalcone Accumulation in Lithocarpus litseifolius Leaves via Regulating Hormonal Crosstalk and Transcriptional Profiling. International Journal of Molecular Sciences, 25(9), 4592.

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Profiling Pears and Kiwifruits' Organic Acids and Polyphenols

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Zaosu pears and Xuxiang kiwifruits were from a local supermarket (Lanzhou, Gansu, China) and were selected for optimal ripeness and the absence of damage or spoilage. The analytical standards used in the organic acid and polyphenol analysis, oxalic, tartaric, l-malic, lactic, citric, succinic, quinic, protocatechuic, chlorogenic, and gallic acids, as well as epi catechin, catechin, caffeic acid, and phloretin, were from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The aromatic compound standards, ethyl acetate, ethyl caproate, ethyl caprylate, isoamyl acetate, hexyl acetate, n-pentanol, phenylethanol, n-hexanol, n-heptanol, β-citronellol, geraniol, linalool, nerol, β-damascenone, and 2-octanol (internal standard), were from Sigma-Aldrich (St. Louis, MO, USA).

Yang X., Song X., Yang L., Zhao J, & Zhu X. (2022). Effect of Deacidification Treatment on the Flavor Quality of Zaosu Pear–Kiwifruit Wine. Foods, 11(14), 2007.

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