Cellsens dimensionr

Manufactured by Olympus

The CellSens DimensionR is a high-performance imaging software solution designed for advanced microscopy applications. It provides a comprehensive set of tools for image acquisition, processing, and analysis, enabling researchers to capture, manage, and interpret complex visual data with precision and efficiency.

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Lab products found in correlation

Dp72 digital camera, by Olympus (4 mentions)

4 protocols using cellsens dimensionr

Histological Analysis of Tissue Samples

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Following collection, tissues were handled as described previously [63 (link)]. All tissues were fixed in 4% paraformaldehyde for 24 hours and transferred into 70% ethanol until processed and embedded in paraffin. Paraffin sections (5 μm) were stained with hematoxylin and eosin (H&E). Pathologists were blinded to vRNA findings when tissue sections were evaluated microscopically. Photomicrographs were obtained using a bright light microscope Olympus BX43 and Olympus BX46 (Olympus Inc., Center Valley, PA) with attached Olympus DP72 digital camera (Olympus Inc.) and Spot Flex 152 64 Mp camera (Spot Imaging) and captured using commercially available image-analysis software (cellSens DimensionR, Olympus Inc. and spot software 5.2).

Crooks C.M., Weiler A.M., Rybarczyk S.L., Bliss M.I., Jaeger A.S., Murphy M.E., Simmons H.A., Mejia A., Fritsch M.K., Hayes J.M., Eickhoff J.C., Mitzey A.M., Razo E., Braun K.M., Brown E.A., Yamamoto K., Shepherd P.M., Possell A., Weaver K., Antony K.M., Morgan T.K., Newman C.M., Dudley D.M., Schultz-Darken N., Peterson E., Katzelnick L.C., Balmaseda A., Harris E., O’Connor D.H., Mohr E.L., Golos T.G., Friedrich T.C, & Aliota M.T. (2021). Previous exposure to dengue virus is associated with increased Zika virus burden at the maternal-fetal interface in rhesus macaques. PLoS Neglected Tropical Diseases, 15(7), e0009641.

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Pathology Assessment of Tissue Samples

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For general pathology, tissues were fixed in 4% PFA, routinely processed and embedded in paraffin. Paraffin sections (5 μm) were stained with hematoxylin and eosin (H&E) or Gram stain using standard methods. Two veterinary pathologists were blinded to vRNA findings when tissue sections were evaluated microscopically. Lesions in each tissue were described and assigned morphologic diagnoses as described previously [55 (link)]. Photomicrographs were obtained using brightfield microscopes Olympus BX43 and Olympus BX46 (Olympus Inc., Center Valley, PA) with attached Olympus DP72 digital camera (Olympus Inc.) and Spot Flex 152 64 Mp camera (Spot Imaging, Sterling Heights, MI), and captured using commercially available image-analysis software (cellSens DimensionR, Olympus Inc. and Spot software 5.3). Uteroplacental pathology was specifically performed by an experienced placental pathologist (T.K.M.).

Mohr E.L., Block L.N., Newman C.M., Stewart L.M., Koenig M., Semler M., Breitbach M.E., Teixeira L.B., Zeng X., Weiler A.M., Barry G.L., Thoong T.H., Wiepz G.J., Dudley D.M., Simmons H.A., Mejia A., Morgan T.K., Salamat M.S., Kohn S., Antony K.M., Aliota M.T., Mohns M.S., Hayes J.M., Schultz-Darken N., Schotzko M.L., Peterson E., Capuano S I.I.I., Osorio J.E., O’Connor S.L., Friedrich T.C., O’Connor D.H, & Golos T.G. (2018). Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection. PLoS ONE, 13(1), e0190617.

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Histological Analysis of Tissue Samples

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Tissues (except neural tissues) were fixed in 4% paraformaldehyde for 24 hours and transferred into 70% ethanol until alcohol processed and embedded in paraffin. Neural tissues were fixed in 10% neutral buffered formalin for 14 days until routinely processed and embedded in paraffin. Paraffin sections (5 µm) were stained with hematoxylin and eosin (H&E). Pathologists were blinded to vRNA findings when tissue sections were evaluated microscopically. Photomicrographs were obtained using a bright light microscope Olympus BX43 and Olympus BX46 (Olympus Inc., Center Valley, PA) with attached Olympus DP72 digital camera (Olympus Inc.) and Spot Flex 152 64 Mp camera (Spot Imaging) and captured using commercially available image-analysis software (cellSens DimensionR, Olympus Inc. and spot software 5.2).

Aliota M.T., Dudley D.M., Newman C.M., Weger-Lucarelli J., Stewart L.M., Koenig M.R., Breitbach M.E., Weiler A.M., Semler M.R., Barry G.L., Zarbock K.R., Haj A.K., Moriarty R.V., Mohns M.S., Mohr E.L., Venturi V., Schultz-Darken N., Peterson E., Newton W., Schotzko M.L., Simmons H.A., Mejia A., Hayes J.M., Capuano S I.I.I., Davenport M.P., Friedrich T.C., Ebel G.D., O’Connor S.L, & O’Connor D.H. (2018). Molecularly barcoded Zika virus libraries to probe in vivo evolutionary dynamics. PLoS Pathogens, 14(3), e1006964.

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Histopathological Evaluation of Tissue Sections

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Tissues were fixed in 4% PFA other than cerebrum, cerebellum and one eye for histology which were fixed in 10% neutral buffered formalin. Tissues were sectioned (~5 mm), routinely processed and embedded in paraffin. Paraffin sections (5 μm and 8 μm for the brain) were stained with HE or Gram stain using standard methods. The pathologists were blinded to vRNA findings when evaluating and describing tissue sections and assigning morphologic diagnoses. Photomicrographs were obtained using brightfield microscopes Olympus BX43 and Olympus BX46 (Olympus Inc., Center Valley, PA) with attached Olympus DP72 digital camera (Olympus Inc.) and Spot Flex 152 64 Mp camera (Spot Imaging, Sterling Heights, MI), and captured using commercially available image-analysis software (cellSens DimensionR, Olympus Inc. and Spot 5.3 Software). The ear diagram used to describe where ear sections were obtained from is a composite of multiple histologic sections of infant 020501 with the addition of an external pinna based on gross images.

Koenig M.R., Razo E., Mitzey A., Newman C.M., Dudley D.M., Breitbach M.E., Semler M.R., Stewart L.M., Weiler A.M., Rybarczyk S., Bach K.M., Mohns M.S., Simmons H.A., Mejia A., Fritsch M., Dennis M., Teixeira L.B., Schotzko M.L., Nork T.M., Rasmussen C.A., Katz A., Nair V., Hou J., Hartman A., Ver Hoeve J., Kim C., Schneider M.L., Ausderau K., Kohn S., Jaeger A.S., Aliota M.T., Hayes J.M., Schultz-Darken N., Eickhoff J., Antony K.M., Noguchi K., Zeng X., Permar S., Prabhakaran V., Capuano S I.I.I., Friedrich T.C., Golos T.G., O’Connor D.H, & Mohr E.L. (2020). Quantitative definition of neurobehavior, vision, hearing and brain volumes in macaques congenitally exposed to Zika virus. PLoS ONE, 15(10), e0235877.

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