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Taqman qpcr reagents

Manufactured by Thermo Fisher Scientific
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TaqMan qPCR reagents are a set of reagents for performing quantitative real-time PCR (qPCR) analysis. The core function of these reagents is to enable the sensitive and accurate detection and quantification of target DNA sequences in a sample.

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Lab products found in correlation

Quantstudio 3 real time pcr system, by Thermo Fisher Scientific (2 mentions) Rneasy plus micro kit, by Qiagen (2 mentions) Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions) Ready to go you prime first strand beads, by Cytiva (1 mentions) Mki67, by Thermo Fisher Scientific (1 mentions) Cenpf, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Superscript reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

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TaqMan reagents (162 citations) TaqMan qPCR (43 citations) QPCR reagents (11 citations)

3 protocols using taqman qpcr reagents

1

RNA Isolation and RT-qPCR Analysis

Cited 2 times
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As previously described,25 (link),26 (link) total RNA was isolated from cells using the RNeasy Plus Micro Kit (QIAGEN, Hilden, Germany) and quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific), and stored at –80°C. The first-strand cDNA was synthesized using Ready-To-Go You-Prime First-Strand Beads (Cytiva Life Sciences, Marlborough, MA, USA), and real-time RT-qPCR was performed using the QuantStudio 3 Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) with TaqMan qPCR reagents (Applied Biosystems). The TaqMan gene expression assays included human glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs99999905-ml), MKI67 (Hs04260396_g1), RRM2 (Hs00357247_g1), TK1 (Hs01062125_m1), CENPF (Hs00193201_m1), NUSAP1 (Hs01006195_m1), UBE2C (Hs00964100_g1), and CDC20 (Hs00415851_g1) (Applied Biosystems). The results were analyzed by the comparative threshold cycle method and normalized by GAPDH.
Li J.M., Kim S., Zhang Y., Bian F., Hu J., Lu R., Pflugfelder S.C., Chen R, & Li D.Q. (2021). Single-Cell Transcriptomics Identifies a Unique Entity and Signature Markers of Transit-Amplifying Cells in Human Corneal Limbus. Investigative Ophthalmology & Visual Science, 62(9), 36.
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2

Gene Expression Analysis of Stem Cell Markers

Cited 1 time
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As previously described [32 ,36 (link)], total RNA was isolated from cells using RNeasy Micro Plus kit (Qiagen), quantified by NanoDrop ND-1000, and stored at −80 °C, the first-strand cDNA was synthesized by using Ready-To-Go You-Prime First-Strand Beads, and the real-time PCR was performed in the Applied Biosystems® QuantStudio® 3 Real-Time PCR System using TaqMan® qPCR reagents with TaqMan gene expression assays including human GAPDH (Hs99999905-ml), TSPAN7 (Hs00190284_m1), SOX17 (Hs00751752_s1), and DCN (Hs00370385_m1) (Applied Biosystems). The results were analyzed by the comparative threshold cycle method and normalized by GAPDH.
Li D.Q., Kim S., Li J.M., Gao Q., Choi J., Bian F., Hu J., Zhang Y., Li J., Lu R., Li Y., Pflugfelder S.C., Miao H, & Chen R. (2021). Single-cell transcriptomics identifies limbal stem cell population and cell types mapping its differentiation trajectory in limbal basal epithelium of human cornea. The ocular surface, 20, 20-32.
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3

Quantitative Expression Analysis of Myogenic Genes

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RNA was extracted from muscle biopsies using TriZol® Reagent (Invitrogen) and from cultured cells using the RNeasy Mini Kit (Qiagen), according to the manufacturers' instructions. cDNA synthesis was performed using random hexamers and SuperScript Reverse Transcriptase (Invitrogen). Quantitative reverse transcriptase PCR (RT-PCR) was performed using Taqman qPCR reagents and an ABI7500 fast system sequencing detection instrument (Applied Biosystems). Primers and probes were assays on demand from Applied Biosystem (Pax7 Hs00242962_m1; MyoD1, Hs00159528_m1; myogenin Hs01072232_m1) and the normalizer gene was glyceraldehyde 3-phosphate dehydrogenase (#4352665, Applied Biosystems).
Franco I., Johansson A., Olsson K., Vrtačnik P., Lundin P., Helgadottir H.T., Larsson M., Revêchon G., Bosia C., Pagnani A., Provero P., Gustafsson T., Fischer H, & Eriksson M. (2018). Somatic mutagenesis in satellite cells associates with human skeletal muscle aging. Nature Communications, 9, 800.
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