Samples were submitted to the California Department of Fish and Wildlife Water Pollution Control Laboratory (Rancho Cordova, CA) for quantification of ibuprofen and caffeine. These samples were analyzed by gas chromatography (Agilent 6890 plus; Agilent Technologies Inc., Santa Clara, CA) with dual columns (DB5 and DB7). The surrogates Gemfibrozil D6 and Sulfamethazine were used with average recoveries of 106.3%, with a range of 83.0% and 123.0% and 84.1% with a range of 71.8% and 94.0%, respectively. The limit of detection for ibuprofen was 32 ng L−1 and 20 ng L−1 for caffeine.
Agilent 6890 plus
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 6890 Plus is a gas chromatography (GC) system designed for analytical and research applications. It features a modular design, advanced temperature control, and precise flow control to deliver reliable performance and accurate results.
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7 protocols using agilent 6890 plus
1
Quantification of Ibuprofen and Caffeine
2
Fatty Acid Profiling for Bacterial Identification
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The selected isolates were cultured in tryptic soy broth medium (TSB) and identified based on the Fatty Acid Methyl Ester (FAME) method based on their fatty acid profiles in the Microbial Identification System (MIDI) of the Sherlock System Software 4.0. The fatty acids were extracted by the four-step method (Mansfeld-Giese, Larsen & Bödker, 2002 ): (i) Saponification, (ii) methylation, (iii) extraction, and (iv) base wash. The fatty acid methyl esters were analyzed in an Agilent 6890 Plus gas chromatograph and identified in the Sherlock System Software 4.0 using the libraries recommended for aerobic heterotrophic bacteria (Parsley, 1996 ).
3
Quantitative Capillary GC Analysis of Residual Crude Oil
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Residual crude oil after extraction (as described in the gravimetric analysis section) at the end of each incubation period was quantified chromatographically via capillary gas chromatography (CGC) using Agilent 6890 plus gas chromatograph equipped with flame ionization detector (FID), split/splitless injector, and fused silica capillary column HP-1 of 30 m length, 0.35 mm internal diameter, and 0.5 μm film thickness. The detector and injector temperatures were maintained at 300°C and 250°C, respectively. The column temperature was programmed to rise from 80°C to 300°C with a rate of 3°C/min and final time 15 min. Nitrogen (free oxygen) was used as a carrier gas at flow rate 2 mL/min.
4
GC-MS Analysis of siAd4BP/SF-1 Knockdown
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Y-1 cells were treated with siAd4BP/SF-1 (#1635) or siControl for 48 h, and then whole cells or mitochondria isolated from the cells were suspended in 0.2 ml methanol at 4 °C. The suspension was sonicated (2 × 20 s pulses, separated by a 30-s interval) on a Bioruptor® Plus (Diagenode). Thereafter, they were centrifuged at 15,000 rpm for 5 min at 4 °C to eliminate methanol-insoluble cellular components. The supernatant (methanol soluble fraction) was recovered and evaporated to remove methanol. Gas chromatography-mass spectrometry analysis was performed using an Agilent 6890 Plus gas chromatograph interfaced with a single-quadrupole Agilent 5975C MSD (Agilent) as previously described49 (link).
5
GC-MS Analysis of Organic Compounds
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Gas chromatography-mass spectrometry (GC-MS) was performed using an Agilent 6890 Plus gas chromatograph interfaced with a single-quadrupole Agilent 5975C MSD (Agilent Technologies, Palo Alto, CA, USA); the electron energy was 70 eV, and the ion source temperature was 230°C. Each sample (2 µL) was injected in split mode (10∶1) at 280°C and separated through a MXT-1 cross-linked dimethylpolysiloxane capillary column (30 m×0.25 mm I.D., 0.25 µm film thickness, Silcosteel-treated stainless steel; Agilent Technologies). The oven temperature was initially set at 260°C for 3 min, increased to 320°C by increasing the temperature at a rate of 10°C/min, increased to 330°C at a rate of 2°C/min and held for 8 min, and finally increased to 380°C at30°C/min rate and held for 3 min. The carrier gas was ultra-high-purity helium at a column head pressure of 75.8 kPa (column flow: 1.1 mL/min, oven temperature: 260°C). For quantitative analysis, the characteristic ions of each compound were determined as their TMS derivatives. Peaks were identified by comparing the retention time and matching the height ratios of characteristic ions.
6
PAHs Extraction and Analysis
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The extraction of PAHs was performed with an acetone/hexane mixture (1:1) through a microwave assisted solvent extraction (Milestone s.r.l., model Ethos D, Sorisole (BG), Italy). The extracted samples were analyzed using an Agilent 6890 PLUS gas chromatograph (Agilent Technologies, Inc., Santa Clara, CA USA) equipped with a programmable temperature vaporization injection system (PTV) and interfaced with a quadrupole mass spectrometer, operating in electron impact ionization (Agilent MS-5973 N). The identification of each PAH (Benzo(a)Anthracene B(a)A, Benzo(b) Fluorene B(b)F, Benzo(jþk)Fluorene B(jþk)F, Benzo(a)Pyrene B(a)P, Benzo(g)Perylene B(g)P, Indeno Pyrene IP and DiBenzoAnthracene DBA) was performed using Perylene-D12 (PrD, 264) as the internal standard (IS). The analytical performance of the whole procedure (extraction recovery, extraction linearity, analytical repeatability, LOD) was verified in our previous study (Bruno et al., 2007) .
7
Fatty acid composition analysis by GC
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The fatty acid composition of the extracted total lipids was determined by gas chromatography (GC) after transmethylation of the samples with 2% H2SO4 in absolute CH3OH at 50°C [25] . Fatty acid methyl esters (FAME) were purified by thin-layer chromatography (TLC) on 20x20 cm plates coated with 0.2 mm silica gel 60 G (Merck) layer with a mobile phase n-hexane: diethyl ether (97: 3, v/ v). Gas chromatographic analysis of the FAME was performed on a GC gas chromatograph Agilent 6890 Plus (Agilent Technologies, Santa Clara, USA) equipped with 5793 mass-selective detectors (Agilent Technologies, Santa Clara CA, USA) and with capillary column SP 2380 (30 mx 0.25 mm x 0.25 µm, Supelco, Bellefonte PA, USA). The column temperature was programmed from 70°C (1 min), at 6°C/min to 190°C (0 min), at 10°C/min to 250°C (0 min); the injector and detector temperatures are maintained at 250°C. Hydrogen was a carrier gas at a 0.8 ml/min flow rate, and the separation was 1: 50. The identification of fatty acids was performed by comparing the retention times with those of a standard mixture of fatty acids subjected to GC under identical experimental conditions [26] .
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