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2 protocols using thc cooh d9

1

Quantification of Cannabinoids in Serum

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Reference standards for THC-d3, THC-OH, THC-OH-d3, THC-COOH-d9, CBD, CBD-d3, CBDA, CBN-d3, CBNA, CBG, CBGA, CBC, CBCA, CBL, THCV, THCVA, CBDV and CBDVA were purchased from Cerilliant (Round Rock, TX, USA), CBG-d9 and CBC-d9 from Cayman Chemical Co. (Ann Arbor, MI, USA) via LGC Standards (Wesel, Germany), CBN, THC and THC-COOH from LGC Standards and THCAA from THC Pharm (Frankfurt a. M., Germany). All standards were certified reference material and had a purity of at least 99.0%, except for THC-OH-d3, THCAA, CBC, CBC-d9, CBCA, CBL, THCVA, CBDV and CBDVA (97.7%), THC-d3 (96.7%), and CBG-d9 (95.0%). All chemicals were obtained from Carl Roth (Karlsruhe, Germany), except for methanol (from Fisher Scientific (Loughborough, UK)) and acetonitrile (from AppliChem (Darmstadt, Germany)). Chemicals used in LC-MS/MS measurement processes were LC-MS grade. All other chemicals were HPLC grade, except for acetone and dichloromethane, which had a purity of 99.8% and 99.5%, respectively. Blank human serum used for calibration was obtained from voluntary healthy blood donors via blood donation service and was tested to be drug-free.
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2

Quantitative Analysis of Cannabinoids

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THC, 11-OH-THC, THCCOOH, CBD, CBN, THC-d3, 11-OH-THC-d3, THCCOOH-d9, CBD-d3, CBN-d3, and THCCOOH-glucuronide-d3 were purchased from Cerilliant (Round Rock, TX, USA). CBG was from Restek (Bellefonte, PA, USA), THCV was from RTI International (Research Triangle Park, NC, USA), and THCVCOOH and THC-glucuronide were acquired from ElSohly Laboratories (Oxford, MS, USA). Ammonium acetate and acetonitrile (LC-MS grade) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methanol and water (LC-MS grade) and formic acid (ACS-grade) were from Fisher Scientific (Fair Lawn, NY, USA). WAX-S tips (1 mL tip containing 20 mg resin and 40 mg salt) were purchased from DPX Labs (Columbia, SC, USA). Chromatography was performed on a Kinetex® C18 column (Phenomenex® Inc., Torrance CA, USA; 2.1 mm × 50 mm, 2.6 μm) combined with a SecurityGuard™ C18 guard column (4 × 2.0 mm).
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