Cytokine production of treated cells was measured by ELISA using the corresponding ELISA kits according to the manufacturers’ protocols. IL-6 (DKW1210602, Dakewe Biotech, China); TNF-α (DKW1217202 Dakewe Biotech, China); IL-10 (CME0016, 4A Biotech, China); TGF-β (DKW1217102 Dakewe Biotech, China). For NO measurement, culture supernatants were harvested after stimulation of macrophages with EST12 protein for indicated time. The release NO level was determined using the NO assay kit (Beyotime, Shanghai, China). Supernatants were added to an equal volume of Griess reagent in duplicate on a 96-well plate and incubated at room temperature for 15 min. Absorbance (540 nm) was measured and NO concentrations were estimated using a standard NO curve. The experiments were performed in triplicate.
Il 10
Manufactured by 4A Biotech
Sourced in China
IL-10 is a recombinant human interleukin-10 protein. Interleukin-10 is a cytokine that regulates inflammatory and immune responses.
Automatically generated - may contain errors
2 protocols using il 10
1
Cytokine and NO Production Assay
2
Cytokine Profiling in Treated Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The blood samples of the mice were collected after 1, 3, 7 and 14 days of treatment. Then, the blood samples were centrifugated at 3000 rpm/min after incubating at 37 °C for 1 h. The levels of IFN-γ (interferon-γ, 4 A Biotech, Beijing, China), TNF-α (tumor necrosis factor-α, 4 A Biotech, Beijing, China) and IL-10 (interleukin-10, 4 A Biotech, Beijing, China) in the serum were analyzed by the ELISA kits according to the manufacturer's protocols.
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