Human CCA cell lines, KKU‐213A, KKU‐213B,27 and KKU‐055 were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank, Osaka, Japan. Cell culture conditions have been described previously.15 This study used NAC, which is a ROS inhibitor, to investigate the effect of high glucose associated with ROS signaling,
Kku 213b
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
The KKU-213B is a laboratory equipment product offered by the Japanese Collection of Research Bioresources Cell Bank. It is a device designed for research purposes, but its core function is not provided in a detailed, unbiased, and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Kku 055, by Japanese Collection of Research Bioresources Cell Bank (6 mentions)
Kku 213a, by Japanese Collection of Research Bioresources Cell Bank (5 mentions)
Kku 100, by Japanese Collection of Research Bioresources Cell Bank (5 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Mmnk 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hucct1, by Thermo Fisher Scientific (1 mentions)
Tfk 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hucca 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Huh 28, by Thermo Fisher Scientific (1 mentions)
Kku213c, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Huh28, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Ssp25, by Thermo Fisher Scientific (1 mentions)
Ham s f12 medium, by Thermo Fisher Scientific (1 mentions)
Hucct1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Reversine, by Cayman Chemical (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
8 protocols using kku 213b
1
Investigating ROS Signaling in High Glucose CCA Cells
2
Cholangiocarcinoma Cell Line Maintenance and Anti-THBS1 Treatment
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Cholangiocarcinoma cell lines (KKU‐055, KKU‐100, KKU‐213A, and KKU‐213B) were obtained from the Japanese Collection of Research Bioresources Cell Bank. All CCA cell lines were grown in low glucose (5 mM) DMEM (LG‐DMEM) (FUJIFILM Wako) supplemented with 10% FBS (Corning) and penicillin/streptomycin (Sigma‐Aldrich) at 37°C with 5% CO2.13 For antibody‐mediated neutralization of THBS1, the cells were treated with anti‐THBS1 antibody (mAB133) (MABT879; Merck) at 1 or 5 μg/ml.
3
Cell Culture Protocols for Cancer Research
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The liver cancer cell line, HepG2 was kindly provided by Dr. Porntip Pinlaor, Associate professor at Faculty of Associated Medical Sciences, Khon Kaen University, Thailand. The cell line was cultured in Eagle's Minimum Essential Medium (EMEM) containing 10% fetal bovine serum and penicillin/streptomycin (100 U/ml and 100 µg/ml) and incubated in a humidified incubator of 5% CO2 at 37 °C. For CCA cell lines, KKU-213B (JCRB1556) was developed by Prof. Banchob Sripa at Cholangiocarcinoma Research Institute, Khon Kaen University, Thailand. In our study, KKU-213B cell lines were purchased from Japanese Collection of Research Bioresources (JCRB). For gastric cancer cell lines, AGS was purchased from the American Type Culture Collection (ATCC). A primary normal fibroblast cell line was purchased from American Type Culture Collection (ATCC). KKU-213B, AGS and normal fibroblast were cultured in Ham’s F-12 containing 10% fetal bovine serum and penicillin/streptomycin (100 U/ml and 100 µg/ml) and incubated in a humidified incubator of 5% CO2 at 37 °C.
4
Immortalized Cholangiocyte and CCA Cell Lines
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The immortalized cholangiocyte cell line (MMNK1) and three human CCA cell lines (KKU-213B, KKU-100, and KKU-055) were obtained from the Japanese Collection of Research Bio-Resources (JCRB) Cell Bank, Japan. All the cells were maintained in DMEM (Dulbecco's Modified Eagle Medium) with high glucose (4.5 g/L) (Gibco, Thermo Fisher, USA) containing 10% fetal bovine serum and 1% penicillin–streptomycin and then kept in an incubator with a 5% CO2 environment at 37 °C. Frozen cell pellets were taken from all cell lines when cell confluency was between 50% and 70% to observe the expression levels of designated miRNAs and expected target genes.
5
Construction of Resistant Cancer Cell Lines
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TFK1, RBE, SSP25, HuCCT1, HuCCA1, HuH28, KKU213A, KKU213B, KKU213C, KKU068, KKU131, KKU138, KKU055, KKU100, and YSCCC cell lines were obtained from the Japanese Collection of Research Bioresources (JCRB; Osaka, Japan). TFK1, RBE, SSP25, and YSCCC cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI 1640) (Gibco; Thermo Fisher Scientific, Waltham, MA, USA). HuCCA1 and KKU100 cells were maintained in Ham’s F-12 medium (Gibco; Thermo Fisher Scientific). HuCCT1, HuH28, KKU213A, KKU213B, KKU213C, KKU068, KKU131, KKU138, and KKU055 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco; Thermo Fisher Scientific). All cell lines were routinely tested for mycoplasma. For the construction of resistant cells, parental cells (KKU213C, TFK1, KKU068, and SSP25) were seeded in 12-well plates at 5 x 104 cells/well in 1 milliliter (ml) of growth medium. Cells were treated with GEM/CIS using a stepwise dose-induction protocol (16 (link)).
6
Culturing CCA and Cholangiocyte Cell Lines
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CCA cell lines (KKU-100, KKU-213A and KKU-213B) were obtained from the Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan and established as previously described (Sripa et al., 2005 (link); Sripa et al., 2020 (link)). An immortalized cholangiocyte cell line, MMNK1 was previously characterized (Maruyama et al., 2004 (link)). Cells were cultured in high glucose (25 mM) Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (FBS) and a 1% antibiotic-antimycotic (Gibco, USA). Cells were incubated at 37 °C in a humidified 5% CO2 atmosphere. At 70–80% confluence, cells were detached from the culture flask using 0.25% w/v trypsin/EDTA and processed according to the particular assay. Reversine was purchased from Cayman Chemical (Michigan, USA) and prepared as a 100 mM stock in DMSO.
7
Establishment and Characterization of Cholangiocarcinoma Cell Lines
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Four CCA cell lines, KKU-055, KKU-100, KKU-213A, and KKU-213B were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) (Sripa et al., 2005 (link); Sripa et al., 2020 (link)). Two metastatic cell lines, KKU-213L5 and KKU-214L5, were established as previously described (Saentaweesuk et al., 2018 (link); Uthaisar et al., 2016 (link)). Cells were maintained in DMEM containing 10% FBS with 1% antibiotic-antimycotic in humidified 5% CO2 at 37 °C. All cell culture-related reagents were obtained from Gibco (NY, USA).
8
Generating Palbociclib-Resistant Cell Lines
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KKU-055 and KKU-213B cell lines were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). The culture methods were previously described (10 (link)). Cell lines were routinely tested for mycoplasma. Palbociclib-resistant cell lines and single-resistant clones were generated as previously described (16 (link), 17 (link)).
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