Serum creatinine, urea, fructosamine and urinary creatinine were analyzed on an ARCHITECT c16000 Clinical Chemistry Analyzer (Abbott Diagnostics, Lake Forest, IL, USA). Glycemia was measured using an AlphaTRAK blood glucose monitoring system (Abbott Laboratories, North Chicago, IL, USA). Serum insulin was assayed by a Rat ultrasensitive ELISA kit (ALPCO, Salem, NH, USA). The presence of glycosuria was defined by a positive Diastix™ (Bayer Corp. Diagnostics, Tarrytown, NY, USA) test (>14mmol/L) on two consecutive mornings.
Architect c16000 clinical chemistry analyzer
Manufactured by Abbott
Sourced in United States
The Architect C16000 Clinical Chemistry Analyzer is a fully automated, high-throughput instrument designed for clinical chemistry analysis. It is capable of performing a wide range of tests, including but not limited to, assays for metabolites, enzymes, proteins, and therapeutic drugs. The analyzer utilizes advanced technologies to provide accurate and reliable results, making it a valuable tool for clinical laboratories and healthcare settings.
Automatically generated - may contain errors
Lab products found in correlation
Nefa kit, by Fujifilm (2 mentions)
Dca vantage analyzer, by Siemens (2 mentions)
Alphatrak blood glucose monitoring system, by Abbott (1 mentions)
Rat ultrasensitive elisa kit, by ALPCO (1 mentions)
Architect system software, by Abbott (1 mentions)
Glycerol standard, by Merck Group (1 mentions)
Triacylglycerol reagent, by Roche (1 mentions)
Xt 4000i automated hematology analyzer, by Sysmex (1 mentions)
S monovette, by Sarstedt (1 mentions)
14 protocols using architect c16000 clinical chemistry analyzer
1
Serum and Urinary Biomarkers in Metabolic Assessment
2
Serum Preparation and Analyte Measurement
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For blood serum preparation collected blood samples were kept at room temperature for 30 min to allow clotting. Serum was collected (top layer) after centrifugation at 2000×g for 10 min at 4 °C. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and triglycerides were measured from serum using an ARCHITECT c16000 Clinical Chemistry Analyzer (Abbott Diagnostics, MA) in the Mount Sinai Clinical Chemistry Laboratory facility according to the manufacturer’s instruction.
3
Biomarkers of Cardiac Dysfunction
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Fasting venous blood was drawn from patients within 24 hours after admission and during the hospitalization and treatment periods. Prealbumin, NT-proBNP, hFABP, and cTnI levels were determined using enzyme-linked immunosorbent assays with commercial kits purchased from Ruilai Bioeng (Beijing, China). The assays were carried out in triplicate according to the manufacturer’s instructions on an ARCHITECT c16000 Clinical Chemistry Analyzer (Abbott, Abbott Park, IL, USA). The detection limit, coefficients of variation, and normal limits for NT-proBNP, hFABP, and cTnI levels were as follows: 3 pg/mL, 5%, and 4000 pg/mL; 1.25 ng/mL, 4%, and 1000 ng/mL; and 25 fg/mL, 4%, and 100 fg/mL; respectively.
4
Creatinine Measurement in Urine
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Measurement of the urinary creatinine concentration was performed at the Mount Sinai Clinical Chemistry Laboratory using an Architect c16000 clinical chemistry analyzer combined with Architect system software (Abbott, IL, USA). The quality control procedure was performed using a liquid unassayed Multiqual control combined with Unity real-time software (Bio-Rad Laboratories, CA, USA). Concentration conversions were performed using Architect system software (Abbott). Urine specific gravity was measured using a J157HA+ automatic refractometer (Rudolph Research Analytical, NJ, USA).
5
Lipid Profile Measurement Protocols
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Serum TC was measured by the colorimetric enzymatic CHOD-PAP method (%CV 134 intra assay: level 1: 0.8, level 2: 0.6; % CV inter assay: level 1: 0.4, level 2: 0.8). HDL-cholesterol was measured by accelerator selective detergent methodology (%CV intra assay: level 1: 1.7, level 136 2: 1; %CV inter assay: level 1: 1.1, level 2: 0.5). TG concentrations were measured by glycerol phosphate oxidase method (% CV intra assay: level 1: 0.7, level 2: 0.8; %CV inter assay: 138 level 1: 0.4, level 2: 0.6). LDL-cholesterol was determined by using Friedewald’s equation: LDL cholesterol = [total cholesterol – (HDL-cholesterol + Triglycerides/5)]. Lipid profile was analyzed by Architect c16000 clinical chemistry analyzer (Abbott, Abbott Park, 130 Illinois, U.S.A.).
6
Comprehensive Cardiometabolic Biomarker Assessment
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Blood samples were drawn from an antecubital vein before coronary angiography after a 12-hour overnight fast and analysed spectrophotometrically on an (auto) Architect C16000 Clinical Chemistry Analyzer (Abbott Inc., Abbott Park, IL, USA) using an enzymatic-colorimetric assay. Fasting blood glucose, blood pressure, hemoglobin A1c (HbA1c), creatinine, fibrinogen, thyroid stimulating hormone (TSH), parathyroid hormone (PTH), hs-CRP, total cholesterol, low-density lipoprotein cholesterol, high-density cholesterol, and triglyceride levels were recorded. 25-hydroxy vitamin D levels were measured using BioSource's 25OH-Vit D3-Ria-CT Kit (BioSource Europe S.A. Rue de L'Industrie, 8, B-1400 Nivelles, Belgium).
For whole blood count (hematocrit, hemoglobin, leukocytes, and platelets), the blood samples were collected in tubes with ethylenediaminetetraacetic acid and analysed on a Coulter LH 780 Hematology Analyzer (Beckman Coulter Ireland Inc., Mervue, Galway, Ireland) device using the impedance and optic scatter method. The body mass index (BMI) was calculated by dividing weight in kg by the square of height in meters (kg/m2).
For whole blood count (hematocrit, hemoglobin, leukocytes, and platelets), the blood samples were collected in tubes with ethylenediaminetetraacetic acid and analysed on a Coulter LH 780 Hematology Analyzer (Beckman Coulter Ireland Inc., Mervue, Galway, Ireland) device using the impedance and optic scatter method. The body mass index (BMI) was calculated by dividing weight in kg by the square of height in meters (kg/m2).
7
Biochemical Markers in Metabolic Health
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Glycosylated hemoglobin (HbA1c) was measured using a DCA Vantage Analyzer (Siemens Medical Solutions Diagnostics, Tarrytown, NY). To measure serum triglycerides, plasma samples as well as glycerol standards (Sigma-Aldrich, St Louis, MO, USA) were incubated with triacylglycerol reagent (Roche Diagnostics) and concentration was determined using the colorimetric method on a Bio-Rad 680 XR (Hercules, CA, USA). Plasma non-esterified fatty acid (NEFA) was measured using a NEFA kit (WAKO, Osaka, Japan). Serum creatinine was measured using the Architect C16000 Clinical Chemistry Analyzer (Abbott Laboratories, Abbott Park, Ill, USA).
8
Comprehensive Metabolic Panel Measurements
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Glycosylated haemoglobin (HbA1c) was measured using a DCA Vantage Analyzer (Siemens Medical Solutions Diagnostics, Tarrytown, NY) [27 (link)]. Serum creatinine and lipid profile including total serum cholesterol, triglycerides and LDL were measured using the Architect C16000 Clinical Chemistry Analyzer (Abbott Laboratories, Abbott Park, Il, USA) available through the affiliated hospital pathology service. Plasma non-esterified fatty acids (NEFA) were measured using a NEFA kit (WAKO, Osaka, Japan) [13 (link)]. Serum insulin was measured using a mouse-specific ELISA method (Merck, Darmstadt, Germany). The density was detected on a Bio-Rad 680 XR (Hercules, CA, USA) [13 (link), 18 (link)]. HOMA-IR was quantified as blood glucose level multiplied by serum insulin level divided by 22.5.
9
Comprehensive Bloodwork Analysis in ED
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Complete blood counts and differentials were studied in the peripheral blood samples: white blood cell (WBC) count, neutrophils, lymphocytes, monocytes, hemoglobin (Hb), platelets, and red cell distribution width (RDW) upon ED admission. Blood samples were taken in calcium-EDTA tubes. CBC were performed with Sysmex XT-4000i-Automated Hematology Analyzer (Sysmex Corporation, Tokyo, Japan). Inflammation-related indexes based on peripheral CBC counts were calculated as follows: the systemic immune inflammation index (SII) = platelet count × neutrophil count/lymphocyte count; the neutrophil‐lymphocyte ratio (NLR) = neutrophil count/lymphocyte count; the monocyte‐lymphocyte ratio (MLR) = monocyte count/lymphocyte count; and the platelet‐lymphocyte ratio (PLR) = platelet count/lymphocyte count. Arterial blood gases, hs-CRP, and other biochemistry parameters were obtained using ABL 90 (Radiometer, Denmark) and ARCHITECT c16000 clinical chemistry analyzer (Abbott Laboratories, Abbott Park, Illinois, USA).
10
Serum Lipid Measurement Protocol
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Venous blood sampling was performed after 12 h of overnight fasting. To obtain serum samples, blood was collected in a S‐Monovette® (Sarstedt AG & Co. KG, Germany) with a silicate clotting activator and polyacrylic gel. After blood collection, the samples were allowed to clot (for 30 min at room temperature) and were then centrifuged (1,500 g; 10 min). Within a maximum of 4 h after blood collection, lipids levels were measured using the automated Architect c16000 Clinical Chemistry Analyzer (Abbott Laboratories, Illinois, USA). Levels of TC (Ref. 7D62‐21), HDL‐C (Ref. 3 K33), LDL‐C (Ref. 1E31‐20), and TG (Ref. 7D74) were measured using an enzymatic spectrophotometric method.
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