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Cpi 613

Manufactured by Merck Group
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CPI-613 is a laboratory reagent produced by Merck Group. It is a pyruvate dehydrogenase complex inhibitor used in research applications. The core function of CPI-613 is to inhibit the pyruvate dehydrogenase complex, a key enzyme involved in cellular metabolism.

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Lab products found in correlation

Sodium dichloroacetate, by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Hibepic, by ScienCell (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Oleic acid, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (1 mentions) Oligomycin a, by Merck Group (1 mentions) L glutamine, by Welgene (1 mentions) Rotenone, by Merck Group (1 mentions) Bptes, by Merck Group (1 mentions) Etomoxir sodium salt hydrate, by Merck Group (1 mentions) Nile red, by Merck Group (1 mentions) Fibroblast growth factor (fgf), by Thermo Fisher Scientific (1 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (1 mentions) Bone morphogenetic protein 4 (bmp4), by Thermo Fisher Scientific (1 mentions) Poly 1 c, by InvivoGen (1 mentions)

3 protocols using cpi 613

1

Cholangiocarcinoma Cell Culture Conditions

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The cells were maintained at 37°C with 5% CO2. Three human CCA cell lines were used: HIBEpic (ScienCell, Carlsbad, CA, USA), HuCCT1 (JCRB, Osaka, Japan) and RBE (The Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China). The cells were maintained in RPMI-1640 medium (Invitrogen, Waltham, MA, USA) containing 10% fetal bovine serum (Biological Industries, Cromwell, CT, USA), penicillin (Invitrogen) (100 U/ml), and streptomycin (Invitrogen) (100 U/ml). N-acetyl-L-cysteine (NAC, A7250) and CPI-613 (S2776) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Selleck (Houston, TX, USA), respectively. Tm (MCE, Monmouth Junction, NJ, USA) was commercially purchased.
Xu L., Wang L., Zhou L., Dorfman R.G., Pan Y., Tang D., Wang Y., Yin Y., Jiang C., Zou X., Wu J, & Zhang M. (2019). The SIRT2/cMYC Pathway Inhibits Peroxidation-Related Apoptosis In Cholangiocarcinoma Through Metabolic Reprogramming. Neoplasia (New York, N.Y.), 21(5), 429-441.
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2

Metabolic Modulation: A Comprehensive Protocol

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Reagents used for experiments: 2-deoxy-d-glucose (D0051, TCI), l-phenylalanine (P0134, TCI), oligomycin A (75351, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), FCCP (C2920, Sigma-Aldrich), sodium pyruvate (11360070, Gibco), sodium dichloroacetate (347795, Sigma-Aldrich), palmitic acid (P0500, Sigma-Aldrich), oleic acid (O1008, Sigma-Aldrich), l-glutamine (LS002-01, Welgene), CPI-613 (SML0404, Sigma-Aldrich), (+)-Etomoxir sodium salt hydrate (E1905, Sigma-Aldrich), BPTES (SML0601, Sigma-Aldrich), nicotinamide riboside chloride (SMB00907, Sigma-Aldrich),
Lee S.R., Roh J.Y., Ryu J., Shin H.J, & Hong E.J. (2022). Activation of TCA cycle restrains virus-metabolic hijacking and viral replication in mouse hepatitis virus-infected cells. Cell & Bioscience, 12, 7.
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3

Metabolic Regulation in Cellular Models

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FBPase-1 inhibitor was purchased from Alfa Aescar, (2E)-3-(3-Pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3-PO) was purchased from Calbiochem, 2-Deoxy-D-glucose (2-DG), Sodium Oxamate, Sodium dichloroacetate (DCA), Dispase I and Nile Red were purchased from Sigma, CPI613, Anacardic Acid (ANAC), BMS303141, SB204990, 8-Br-cAMP and SB431542 were purchased from Tocris Bioscience. PolyI:C was from InvivoGen, VEGF, FGF, BMP4 were from Pepro Tech. U13C-glucose was from Cambridge Isotope Laboratories. Human specific-CD31 was purchase from BD, ACL antibody used for IF was from Thermo Fisher, SLC25A1 antibody was from Proteintech, and the other antibodies were from Cell Signaling Technology. TMRM was from Invitrogen. siControl and siACL were from Dharmacon. Lipofectamine 3000 transfection reagents were from Invitrogen. Oxidative stress and Glycolytic stress assays and were purchased from Seahorse Bioscience. Growth factor reduced Matrigel was from BD. Glucose uptake and lactake production assay kit were from Cayman Chemical. Nuclear/Cytosol fraction kit was from BioVision.
HAT activity assay kit was from EpiGentek. QBT fatty acid uptake assay kit was from Molecular Devices.
Lai L., Reineke E., Hamilton D.J, & Cooke J.P. (2019). A Glycolytic Switch is Required for Transdifferentiation to Endothelial Lineage. Circulation, 139(1), 119-133.
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