Rabbit anti mic60

The following antibodies were used in this study: rabbit anti-Myo19 (HPA059715, 1:1000 for western blotting) from Sigma-Aldrich; rabbit anti-Mic60 (A2751, 1:3000 for western blotting), anti-Metaxin2 (A7958, 1:1000 for western blotting) and rabbit anti-Sam50 (A3401, 1:3000 for western blotting, 1:400 for PLA assay) from ABclonal; mouse anti-Mic60 (sc-390707, 1:200 for immunofluorescence staining) from Santa Cruz Biotechnology; rabbit anti-GFP (598, 1:1000 for western blotting, 1:200 for immunofluorescence staining) and mouse anti-GFP (MO48-3, 1:200 for immunofluorescence staining) from MBL; anti-mouse (sc-516102, 1:4000) and anti-rabbit (sc-2004, 1:4000) horseradish peroxidase (HRP)-conjugated secondary antibodies from Santa Cruz Biotechnology; Alexa Fluor488- or Alexa Fluor 555-conjugated secondary antibody from ThermoFisher Scientific. For reagents, MitoTracker^TM Red CMXRos (M7512), MitoTracker^TM Green FM (M7514), TMRE (T669), TMRM (T668) and Prolong Diamond Antifade with DAPI (P36962) were purchased from Thermo Fisher Scientific. Rotenone (HY-B1756), FCCP (HY-100410) and 2-Deoxy-D-glucose (HY-13966) were purchased from MedChemExpress.

The following antibodies were used in this study: rabbit anti-Myo19 (HPA059715, 1:1000 for western blotting and 1:800 for IHC) and mouse anti-α-tubulin (T9026, 1:4000 for western blotting) from Sigma-Aldrich; rabbit anti-Mic60 (A2751, 1:1000 for western blotting), rabbit anti-Src (A19119, 1:1000 for western blotting) rabbit anti- pSrc Y416 (AP0452, 1:1000 for western blotting) from ABclonal; mouse anti-RhoA (ARH04, 1:800 for western blotting) from Cytoskeleton; rabbit anti-GAPDH (ab181602, 1:4000 for western blotting) from Abcam; mouse anti-Nrf2 (sc-365949, 1:800 for western blotting) from Santa Cruz; anti-mouse (sc-516102, 1:4000) and anti-rabbit (sc-2004, 1:4000) horseradish peroxidase (HRP)-conjugated secondary antibodies from Santa Cruz Biotechnology.
For reagents, CellTracker™ Red CMTPX (C34552), CellTracker™ Green CMFDA (C2925) and MitoSOX (M36008) were purchased from ThermoFisher Scientific. 2-Deoxy-D-glucose (HY-13966) was purchased from MedChemExpress. H₂O₂ (H112515), catalase (C163049), and MitoPY (M286913) were purchased from Aladdin. PP2 (SC1234) was purchased from Beyotime. Rho activator II (CN03) was purchased from Cytoskeleton. Dasatinib (S45672) was purchased from Yuanye. MitoTEMPO (S9733) was purchased from Selleck. Ehop-016 (BD559120) was purchased from Bidepharm. AntimycinA (ab141904) was purchased from Abcam.