Calcium chloride dihydrate

Manufactured by Vetec

Sourced in Brazil

Calcium chloride dihydrate is a chemical compound with the formula CaCl2·2H2O. It is a crystalline solid that is highly soluble in water. The primary function of calcium chloride dihydrate is to serve as a desiccant, or a substance that absorbs moisture from the surrounding environment.

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Calcium chloride (6 citations)

5 protocols using calcium chloride dihydrate

Isolated Vascular Tissue Preparation

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Calcium chloride dihydrate (CaCl₂·2H₂O), potassium chloride (KCl), and sodium bicarbonate (NaHCO₃) were purchased from Vetec (Rio de Janeiro, RJ, Brazil). Glucose (C₆H₁₂O₆), magnesium sulfate heptahydrate (MgSO₄·7H₂O), hydrochloric acid (HCl), and monobasic potassium phosphate (KH₂PO₄) were from Nuclear (Porto Alegre, RS, Brazil). Sodium chloride (NaCl) was purchased from Dinâmica (Diadema, SP, Brazil) and acetylcholine chloride (ACh) from Merck (Brazil). Phenylephrine (PHE) was obtained from Pfizer (USA), and Nω-nitro-L-arginine methyl ester (L-NAME) was purchased from Sigma-Aldrich (Brazil). Ethylenediamine tetraacetic acid (EDTA) (1 : 250) came from BioTécnica-Advanced Biotechnology (Brazil), and carbogen mixture (95% O₂ and 5% CO₂) was obtained from White Martins (Brazil). All substances were weighed on an analytical balance, GEHAKA model AG 200 (Sao Paulo, SP, Brazil).

de Freitas Brito A., Silva A.S., de Souza A.A., Ferreira P.B., de Souza I.L., da Cunha Araujo L.C., da Silva Félix G., de Souza Sampaio R., da Conceição Correia Silva M., Tavares R.L., de Andrade Pereira R., Neto M.M, & Silva B.A. (2019). Supplementation with Spirulina platensis Modulates Aortic Vascular Reactivity through Nitric Oxide and Antioxidant Activity. Oxidative Medicine and Cellular Longevity, 2019, 7838149.

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Preparation of Physiological Solutions

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Calcium chloride dihydrate (CaCl₂.2H₂O), magnesium chloride
hexahydrate (MgCl₂.6H₂O), potassium chloride (KCl) and sodium
bicarbonate (NaHCO₃) were purchased from VETEC (Brazil). Monosodium phosphate
1-hydrate (NaH₂PO₄.H₂O), glucose
(C₆H₁₂O₆), magnesium sulfate monohydrate
(MgSO₄.H₂O) and hydrochloric acid (HCl) were purchased from
Nuclear (Brazil). Sodium chloride (NaCl) was purchased from Dinâmica (Brazil).
Carbamylcholine chloride (CCh), aminophylline, trichloroacetic acid, arachidonic acid
(AA), and thiobarbituric acid were purchased from Sigma-Aldrich (Brazil). Carbogenic
mixture (95% O₂ and 5% CO₂) was purchased from White Martins
(Brazil).

Brito A.F., Silva A.S., Souza I.L., Pereira J.C., Martins I.R, & Silva B.A. (2015). Intensity of swimming exercise influences tracheal reactivity in rats. Journal of Smooth Muscle Research, 51, 70-81.

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Rat Aortic Vascular Reactivity

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Calcium chloride dihydrate (CaCl₂.2H₂O), magnesium chloride
hexahydrate (MgCl₂.6H₂O), potassium chloride (KCl), and sodium
bicarbonate (NaHCO₃) were purchased from VETEC (Brazil). Monosodium
phosphate 1-hydrate (NaH₂PO₄.H₂O), glucose
(C₆H₁₂O₆), magnesium sulfate monohydrate
(MgSO₄.H₂O), and hydrochloric acid (HCl PA) were purchased
from Nuclear (Brazil). Sodium chloride (NaCl) was purchased from Dinâmica (Brazil).
Carbamylcholine chloride, acetylcholine (ACh), and phenylephrine (Phe) were purchased
from Sigma-Aldrich (USA). The carbogenic mixture (95% O₂ and 5%
CO₂) was purchased from White Martins (Brazil).

Brito A.F., Silva A.S., Souza I.L., Pereira J.C, & da Silva B.A. (2015). Intensity of swimming exercise influences aortic reactivity in rats. Brazilian Journal of Medical and Biological Research, 48(11), 996-1003.

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Vascular Reactivity Assay Reagents

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Calcium chloride dihydrate (CaCl₂·2H₂O), magnesium sulfate heptahydrate (MgSO₄·7H₂O), and glucose (C₆H₁₂O₆) were purchased from Vetec (Rio de Janeiro, RJ, Brazil). Sodium bicarbonate (NaHCO₃), sodium chloride (NaCl), and potassium chloride (KCl) were purchased from Nuclear (Porto Alegre, RS, Brazil). Monobasic potassium phosphate (KH₂PO₄), monobasic sodium phosphate (NaH₂PO₄), sodium hydroxide (NaOH), and hydrochloric acid (HCl) were purchased from Dinâmica (Diadema, SP, Brazil).
Phenylephrine (PHE) was purchased from Pfizer (USA). ACh, R-(-)-apomorphine, Nω-nitro-L-arginine methyl ester (L-NAME), indomethacin, tempol, apocinin, ethylenediamine tetraacetic acid (EDTA), MDA, and 1,1-diPHEnyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (Brazil). To obtain the stock solutions, substances used in the functional experiments were dissolved and diluted in distilled water (indomethacin and apocinin were dissolved in absolute alcohol 96° GL), being, respectively, kept at 4 or -20°C. The carbogen mixture (95% O₂ and 5% CO₂) was obtained from White Martins (Brazil). All substances were weighed on an analytical balance, GEHAKA model AG 200 (Sao Paulo, SP, Brazil).

Diniz A.F., de Souza I.L., dos Santos Ferreira E., de Lima Carvalho M.T., Barros B.C., Ferreira P.B., da Conceição Correia Silva M., Júnior F.F., de Lima Tavares Toscano L., Silva A.S., de Andrade Cavalcante F, & da Silva B.A. (2020). Potential Therapeutic Role of Dietary Supplementation with Spirulina platensis on the Erectile Function of Obese Rats Fed a Hypercaloric Diet. Oxidative Medicine and Cellular Longevity, 2020, 3293065.

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Curcumin and Quercetin Topical Formulation

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Curcumin (CUR), quercetin (QU), cetalkonium chloride, deacetylated gellan gum (Gelzan™ CM), polyethylene glycol stearate 660 (Solutol HS15^®), castor oil, and porcine stomach mucin (type II) were bought from Sigma–Aldrich (St. Louis, MO, USA). Egg phospholipids with 80% phosphatidylcholine (Phospholipon E80^®) and purified fish oil (DHA/EPA) were obtained from Lipoid (Ludwigshafen, Germany). Acetonitrile of high-performance liquid chromatography (HPLC) grade was purchased from Panreac (Barcelona, Spain), and the pure water was achieved with a Milli-Q^® water system (Millipore, Burlington, MA, USA). Polyethylene glycol 400 (PEG400), potassium chloride, and sodium chloride were obtained from Synth (São Paulo, SP, Brazil). Monobasic sodium phosphate and dibasic sodium phosphate were purchased from Alphatec (Porto Alegre, RS, Brazil). The calcium chloride dihydrate was purchased from Vetec (Rio de Janeiro, RJ, Brazil). Ethanol, acetone, phosphoric acid, and other reagents were of analytical grade.

Vaz G.R., Carrasco M.C., Batista M.M., Barros P.A., Oliveira M.D., Muccillo-Baisch A.L., Yurgel V.C., Buttini F., Soares F.A., Cordeiro L.M., Fachel F., Teixeira H.F., Bidone J., de Oliveira P.D., Sonvico F, & Dora C.L. (2022). Curcumin and Quercetin-Loaded Lipid Nanocarriers: Development of Omega-3 Mucoadhesive Nanoemulsions for Intranasal Administration. Nanomaterials, 12(7), 1073.

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