Recombinant human bmp9

Manufactured by R&D Systems

Sourced in United Kingdom, United States, Germany

Recombinant human BMP9 is a protein produced in E. coli cells. It is a member of the bone morphogenetic protein family, which are involved in bone and cartilage development. The core function of Recombinant human BMP9 is to induce osteoblast differentiation and bone formation.

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Lab products found in correlation

Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Romidepsin, by Selleck Chemicals (1 mentions) Rgfp966, by Selleck Chemicals (1 mentions) Tubacin, by Merck Group (1 mentions) Recombinant human bmp6, by R&D Systems (1 mentions) Pci 34051, by Selleck Chemicals (1 mentions) Lmk 235, by Selleck Chemicals (1 mentions) Ldn193189, by Merck Group (1 mentions) Normal goat igg, by R&D Systems (1 mentions) Ldn193189, by Axon Medchem (1 mentions) Trypsin edta treatment, by Thermo Fisher Scientific (1 mentions) Cd31 microbead kit, by Miltenyi Biotec (1 mentions) Rlt buffer, by Qiagen (1 mentions) Sew2871, by Cayman Chemical (1 mentions) Nibr 0213, by Cayman Chemical (1 mentions) Sphingosine 1 phosphate (s1p), by Cayman Chemical (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Sch772984, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Recombinant BMP9 Human BMP9

6 protocols using recombinant human bmp9

Isolation and Culture of Human BOECs

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Human blood outgrowth endothelial cells (BOECs) were isolated from peripheral
blood of PAH patients and healthy controls as previously described.^{28 (link)–30 (link, link)} All blood donors provided
informed consent in accordance with the human study protocol – 07/H0306/134
(Cambridgeshire 3 Research Ethics Committee). Cells were cultured and expanded
in Endothelial Cell Growth Medium-2 (EGM-2) (minus heparin) (Lonza, Slough, UK)
with 10% Foetal Bovine Serum (FBS) (Thermo Fisher Scientific, Hemel Hempstead,
UK). All experiments were performed with passage 5–7 cells. Human or mouse
pulmonary artery smooth muscle cells (PASMCs) were isolated as previously
described and cultured in DMEM (Invitrogen) containing 20% (v/v) FBS and A/A
(DMEM/20% FBS).^{31 (link)} The Royal Papworth Hospital ethical review committee approved the use of
the human tissues (Ethics Ref 08-H0304-56 + 5), and informed consent was
obtained from all subjects. Recombinant human BMP9 was purchased from R&D
Systems (Oxfordshire, UK). PTC124 was a kind gift from PTC Therapeutics (South
Plainfield, NJ, USA) and was used at 100 µM overnight in all in vitro
experiments.

Long L., Yang X., Southwood M., Moore S., Crosby A., Upton P.D., Dunmore B.J, & Morrell N.W. (2020). Targeting translational read-through of premature termination mutations in BMPR2 with PTC124 for pulmonary arterial hypertension. Pulmonary Circulation, 10(3), 2045894020935783.

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Preparation of HDAC Inhibitors and BMPs

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PB (LC Laboratories, Woburn Massachusetts, USA) was reconstituted in DMSO and, for mouse experiments was diluted in vehicle (H₂O with tween 5%, PEG 5%). Specific HDAC inhibitors were reconstituted in DMSO: Romidepsin (Selleck), RGFP966 (Selleck), LMK235 (Selleck), Tasinquimod (Selleck), Tubacin (Sigma), PCI-34051 (Selleck). Recombinant Human BMP6 (R&D systems) and Recombinant Human BMP9 (R&D systems) were reconstituted in sterile 4 mM HCl containing 0.1% bovine serum albumin. LDN193189 (Sigma) was diluted in H₂O.

Pasricha S.R., Lim P.J., Duarte T.L., Casu C., Oosterhuis D., Mleczko-Sanecka K., Suciu M., Da Silva A.R., Al-Hourani K., Arezes J., McHugh K., Gooding S., Frost J.N., Wray K., Santos A., Porto G., Repapi E., Gray N., Draper S.J., Ashley N., Soilleux E., Olinga P., Muckenthaler M.U., Hughes J.R., Rivella S., Milne T.A., Armitage A.E, & Drakesmith H. (2017). Hepcidin is regulated by promoter-associated histone acetylation and HDAC3. Nature Communications, 8, 403.

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Investigating ALK1 Receptor Fusion Protein

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ALK1Fc (de Vinuesa et al., 2016 (link)) is a fusion protein comprised of the extracellular domain (ECD) of human ALK1 fused to the Fc region of IgG and was obtained from Acceleron Pharma, Cambridge, USA. As a control we used either the Fc domain of IgG₁ (MOPC-21; Bio Express, West Lebanon NH) or normal goat IgG from R&D System.
Recombinant human BMP9 was obtained from R&D System, whereas the chemical inhibitor LDN193189 was purchased from Axon Medchem. The final concentration for the in vitro experiments was 1 nM for BMP9 and 120 nM for LDN193189.

Astrologo L., Zoni E., Karkampouna S., Gray P.C., Klima I., Grosjean J., Goumans M.J., Hawinkels L.J., van der Pluijm G., Spahn M., Thalmann G.N., ten Dijke P, & Kruithof-de Julio M. (2017). ALK1Fc Suppresses the Human Prostate Cancer Growth in in Vitro and in Vivo Preclinical Models. Frontiers in Cell and Developmental Biology, 5, 104.

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Isolation of Endothelial Cells from Feeder Cell Co-cultures

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Feeder cells (fibroblasts, MSCs, SMCs and pericytes) were seeded into a 6-well plate and cultured until confluent. Then, 1.5×10⁵ endothelial cells (HUVECs or HAECs) were seeded into these feeder cells or into a collagen-coated Boyden chamber (0.4 µm pore, BD). The next day, the culture media were replaced with HuMedia and the cells were treated with recombinant human BMP9 (10 ng/ml) (R&D systems). After an 18-22 h incubation with BMP9, the cells were harvested by trypsin/EDTA treatment (Life Technologies) and HUVECs were separated from fibroblasts or MSCs by magnetic cell sorting (MACS) using a CD31 MicroBead Kit (Miltenyi Biotech) following the manufacturer's instructions. For Notch pathway inhibition, γ-secretase inhibitor IX (1 µM) (Calbiochem) was added 15 min before BMP9 treatment. The collected cells were lysed in buffer RLT (Qiagen) for RNA purification.

Tachida Y., Izumi N., Sakurai T, & Kobayashi H. (2017). Mutual interaction between endothelial cells and mural cells enhances BMP9 signaling in endothelial cells. Biology Open, 6(3), 370-380.

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Signaling Pathway Regulation Protocol

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The reagents and chemicals were mostly purchased from Thermo Fisher Scientific (Shanghai, China) unless otherwise indicated. S1P, SEW2871, NIBR0213, FTY720, and W146 were purchased from Cayman Chemical (Ann Arbor, MI, USA). Recombinant human BMP9 was purchased from R&D systems (Minneapolis, MN, USA). The ERK inhibitor SCH772984 and the AKT (protein kinase-B, PKB, also called AKT) inhibitor MK-2206 were purchased from Selleck (Shanghai, China).

Wang B., Dong N., Wu D., Fang Y., Chen J., Lin Y., Bellusci S., Zhang J.S., Dai K, & Chen C. (2022). Sphingosine 1-phosphate receptor 1 governs endothelial barrier function and angiogenesis by upregulating endoglin signaling. Annals of Translational Medicine, 10(3), 136.

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Growth Factor Purification and Preparation

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Recombinant human BMP-9, BMP-2, BMP-6 and mouse hepatocyte growth factor (HGF) were from R&D Systems (Wiesbaden-Nordenstadt, Germany) and recombinant human TGF-β1 and platelet derived growth factor (PDGF)-B were from Peprotech (Rocky Hill, USA).

Breitkopf-Heinlein K., Meyer C., König C., Gaitantzi H., Addante A., Thomas M., Wiercinska E., Cai C., Li Q., Wan F., Hellerbrand C., Valous N.A., Hahnel M., Ehlting C., Bode J.G., Müller-Bohl S., Klingmüller U., Altenöder J., Ilkavets I., Goumans M.J., Hawinkels L.J., Lee S.J., Wieland M., Mogler C., Ebert M.P., Herrera B., Augustin H., Sánchez A., Dooley S, & Ten Dijke P. (2017). BMP-9 interferes with liver regeneration and promotes liver fibrosis. Gut, 66(5).

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