The morphological examination of the optimized NLCs was performed by transmission electron microscopy (TEM) using a JEOL 1010 Instrument (Jeol Inc., Peabody, MA, USA), with a 60,000× magnification. A drop of the sample (without previous dilution) was added onto a copper grid coated with carbon film and negative stained with uranyl acetate solution 2%. The grids were dried around 24 h for later reading [22 (link)].
1010 instrument
Manufactured by JEOL
Sourced in United States, Japan
The JEOL 1010 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a LaB6 electron source, advanced optics, and a digital imaging system. The JEOL 1010 is capable of providing detailed structural and compositional information at the nanometer scale.
Automatically generated - may contain errors
3 protocols using 1010 instrument
1
Nanostructured Lipid Carriers Morphology
2
Characterization of m@V-SiO2-P Nanoparticles
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The morphologies of the m@V-SiO2-P/LB and m@V-SiO2-P nanoparticles were observed via transmission electron microscopy (TEM) and scanning electron microscopy (SEM). For TEM observations, samples were suspended in water, dried on a carbon film-supported copper grid, and observed using a JEOL 1010 instrument (JEOL, Tokyo, Japan) operated at 100 kV. For SEM measurements, samples were dropped onto silicon wafers, dried, and characterized using field-emission scanning electron microscopy (FE-SEM) with a JEOL JSM 7800 microscope (JEOL, Tokyo, Japan). The particle size and zeta potential were measured using dynamic light scattering (DLS) with a Zetasizer Nano-ZS instrument.
3
Synthesis and Characterization of Citrate-Capped Silver Nanoparticles
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Citrate-capped AgNPs were produced in deionized water (a Deionizer Millipore Simplicity system) by the citrate reduction method, adapted from Ranoszek-Soliwoda et al. (2017) . Briefly, 1 mM AgNO 3 (99% purity; Fluka) and sodium citrate solution (C 6 H 5 Na 3 O 7 •2H 2 O, purity 99%; Sigma-Aldrich) were heated in a heating mantle in a round-bottomed flask for approximately 15 min under vigorous stirring. Tannic acid (C 76 H 52 O 46 ; Sigma-Aldrich) was introduced (dropwise), and the mixture was further heated for another 15 min. A constant molar ratio of AgNO 3 to sodium citrate and Tannic acid (1:7:2) was maintained throughout the synthesis. The final concentration of the AgNP dispersion was 100 µg/mL. Transmission electron microscopy analyses were carried out on a JEOL 1010 instrument (Supplemental Data, Figure S1). Dynamic light scattering analysis was carried out to determine the NP size and zeta potential. The particle size ranged from 10 to 50 nm, with an average (±standard deviation [SD] , n = 276) size of 32.88 ± 9.2 nm (Supplemental Data, Figure S1). The zeta potential was approximately -32 mV. The AgNO 3 used as the ionic counterpart in the toxicity tests was purchased from Sigma-Aldrich (≥99% purity).
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