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Anti jun

Manufactured by Proteintech
Sourced in United States

The Anti-JUN product is a laboratory reagent that detects the JUN protein, which is a transcription factor involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to identify and quantify the JUN protein in biological samples.

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2 protocols using anti jun

1

Whole Cell Lysate Protein Analysis

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Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer (Beyotime, P0013E) with a freshly added protease inhibitor tablet (Thermo Scientific, 88265). Western blot analyses were performed with anti-β-actin (Kangcheng, KC-5A08), anti-α-Tubulin (Proteintech, 11224-1-AP), anti-JUN (Proteintech, 24909-1-AP), anti-CEBPB (Proteintech, 23431-1-AP), anti-HDAC3 (Proteintech, 10255-1-AP), anti-HIF-1α(Proteintech, 20960-1-AP), and anti-MGMT (Proteintech, 17195-1-AP) antibodies.
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2

Fbw7 Regulation of Stat3 Signaling

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The following antibodies were used in the study: anti-Fbw7 (for IHC) (H00055294-M02, Abnova, Taipei City, Taiwan), anti-Fbw7 (for western blots) (ab109617, Abcam, Cambridge, UK), anti-Stat3, 12640; anti-phospho-Stat3Tyr705, 4113; anti-Ubiquitin, 3936 (Cell Signaling Technology, Beverly, MA, USA), anti-Myc tag, 16286–1-AP; anti-Flag tag, 66008–2-Ig; and anti-β-actin, 60008–2-Ig; anti-Myc, 10828–1-AP; anti-Notch, 10062–2-AP; anti-Jun, 10024–2-AP; anti-DEK, 16448–1-AP; anti-Mcl1, 16225–1-AP(Proteintech, Rosemont, IL, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Amresco (Dallas, TX, USA).
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