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Pharmacia ultrospec 2000 uv vis spectrophotometer

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The Pharmacia Ultrospec 2000 UV-Vis spectrophotometer is a laboratory instrument used for the measurement of the absorbance of light by samples in the ultraviolet and visible light spectrum. It is designed to analyze the optical properties of a wide range of liquid samples.

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Ultrospec 2000 UV/VIS Ultrospec 2000 spectrophotometer UV-Vis spectrophotometer Ultrospec 2000 UV spectrophotometer

3 protocols using pharmacia ultrospec 2000 uv vis spectrophotometer

1

Photosynthetic Pigment Quantification

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Cell samples were harvested by centrifugation (8000 g, 15 min), and the extraction of photosynthetic pigments was performed with absolute methanol according to [21 (link)]. The extracts were manipulated under dim green light to avoid pigment photo-degradation and were measured with a Pharmacia Ultrospec 2000 UV-Vis spectrophotometer (1-nm bandwidth; Amersham Biosciences, Piscataway, NJ, USA) at 665 nm (Chl a), 653 nm (Chl b), 470 nm (Cars) and 750 nm (background disturbance). Pigment concentrations were quantified as reported in Wellburn [91 (link)] and expressed as μgpigment 10−6 cells.
Baldisserotto C., Demaria S., Accoto O., Marchesini R., Zanella M., Benetti L., Avolio F., Maglie M., Ferroni L, & Pancaldi S. (2020). Removal of Nitrogen and Phosphorus from Thickening Effluent of an Urban Wastewater Treatment Plant by an Isolated Green Microalga. Plants, 9(12), 1802.
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2

Quantifying Photosynthetic Pigments in Leaves

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Photosynthetic pigments were extracted from pre-weighted small round pieces of leaflets and stipules with a surface area of 28.27 mm 2 . Extraction was performed with 100% methanol for 10 min at 80 °C and pigment concentration was determined according to Wellburn [53] using a Pharmacia Ultrospec 2000 UV-Vis spectrophotometer (1 nm bandwidth) (Amersham Biosciences, Piscataway, NJ, USA). Pigments were quantified both on surface area unit and on fresh weight unit basis. At least four replicates were analysed for both leaflets and stipules.
Giovanardi M., Pantaleoni L., Ferroni L., Pagliano C., Albanese P., Baldisserotto C, & Pancaldi S. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochimica et biophysica acta. Bioenergetics, 1859(10).
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3

Photosynthetic Pigment Quantification

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For photosynthetic pigment analysis, cell samples were col-lected by centrifugation after 0, 7, 17, 21, and 25 days of cultivation. Extraction of photosynthetic pigments was per-formed according to Baldisserotto et al. (2014) . The extracts were measured with a Pharmacia Ultrospec 2000 UV-Vis spectrophotometer (1-nm bandwidth; Amersham Biosciences, Piscataway, NJ, USA) at 666 (chlorophyll a (Chla)), 653 (chlorophyll b (Chlb)), and 470 nm (carotenoids (Car)). Quantification was performed according to equations reported in Wellburn (1994) .
Sabia A., Baldisserotto C., Biondi S., Marchesini R., Tedeschi P., Maietti A., Giovanardi M., Ferroni L, & Pancaldi S. (2015). Re-cultivation of Neochloris oleoabundans in exhausted autotrophic and mixotrophic media: the potential role of polyamines and free fatty acids. Applied microbiology and biotechnology, 99(24).
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