Ecfp ssh1δc

Manufactured by Addgene

ECFP-SSH1ΔC is a plasmid vector that expresses the Enhanced Cyan Fluorescent Protein (ECFP) fused to the C-terminal deletion mutant of the SSH1 (Slingshot-1) protein. The core function of this product is to enable the visualization and study of the subcellular localization of the SSH1 protein in living cells through fluorescent microscopy.

Automatically generated - may contain errors

Lab products found in correlation

Poptn egfp, by Addgene (2 mentions) Pbabe puro mcherry egfp lc3b, by Addgene (1 mentions) Pn3 3xflag control, by Addgene (1 mentions) Pbabe egfp, by Addgene (1 mentions) Mkeima red mito 7, by Addgene (1 mentions) Ha ubiquitin, by Addgene (1 mentions) Pmrfp lc3, by Addgene (1 mentions) Pmxs puro gfp p62, by Addgene (1 mentions) Pegfp n1 human cofilin wt, by Addgene (1 mentions)

2 protocols using ecfp ssh1δc

Autophagy and Actin Dynamics Regulation

Cited 3 times

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pN3-3xFlag-Control (Addgene, 107717, Guntram Suske Lab [75 (link)]); pmRFP-LC3 (Addgene, 21075, Tamotsu Yoshimori Lab [43 (link)]); pMXs-puro GFP-Sqstm1/p62 (Addgene, 38277, Noboru Mizushima Lab [49 (link)]); pBABE-puro mCherry-EGFP-LC3B (Addgene, 22418, Jayanta Debnath Lab [76 (link)]); pBABE-EGFP (Addgene, 36999, Debu Chakravarti Lab [77 (link)]); ECFP-SSH1ΔC (N461) (Dr. Mizuno lab [51 (link)]); EGFP-SSH1 (Dr. Storz lab [69 (link)]); mKeima-Red-Mito-7 (Addgene, 56018); pOPTN-EGFP (Addgene, 27052Beatrice Yue Lab [78 (link)]); pMXs-puro GFP-Sqstm1/p62ΔC (Addgene, 38282, Noboru Mizushima Lab [49 (link)]); pMXs-puro GFP-Sqstm1/p62 D337, 338, 339A (GFP-SQSTM1-LIR) (Addgene, 38280, Noboru Mizushima Lab [49 (link)]); HA-Ubiquitin (Addgene, 18712, Edward Yeh Lab [79 (link)]); pDR125 (Addgene, 37150, Dale Ramsden Lab); pN3-3xFlag-SSH1, mCherry-EGFP-Sqstm1/p62, mCherry-EGFP-Sqstm1^S403A, mCherry-EGFP-Sqstm1^S403E, pN3-Flag-SSH1ΔC, pN3-Flag-SSH1ΔN and pN3-Flag-SSH1ΔN^C393S were generated in this work (see methods below).

Fang C., Woo J.A., Liu T., Zhao X., Cazzaro S., Yan Y., Matlack J., Kee T., LePochat P, & Kang D.E. (2020). SSH1 impedes SQSTM1/p62 flux and MAPT/Tau clearance independent of CFL (cofilin) activation. Autophagy, 17(9), 2144-2165.

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Plasmid Constructs for Autophagy and Cytoskeleton Research

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

pMXs-puro GFP-p62 (Addgene, 38277) (Itakura and Mizushima, 2011 (link)), pMXs-puro GFP-p62ΔC (addgene, 38282) (Itakura and Mizushima, 2011 (link)), ECFP-SSH1ΔC (Kurita et al., 2008 (link)), pEGFP-N1 human cofilin WT (addgene, 50859) (Garvalov et al., 2007 (link)), pEGFP-N1 human cofilin S3A (addgene, 50860) (Garvalov et al., 2007 (link)), pEGFP-N1 human cofilin S3E (addgene, 50861) (Garvalov et al., 2007 (link)), pOPTN-EGFP (addgene, 27052) (Park et al., 2006 (link)), pOPTN E478G-EGFP (addgene, 68848) (Turturro et al., 2014 (link)) were obtained from corresponding sources. Plasmids p3xFlag-SSH1 and p3xFlag-SSH1ΔN, and GFP-p62 403E were generated in the Kang lab as previously documented (Fang et al., 2021 (link)).

Cazzaro S., Fang C., Khan H., Witas R., Kee T.R., Woo J.A, & Kang D.E. (2022). Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Frontiers in Aging Neuroscience, 14, 933979.

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