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Coherent opal photoactivator

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The Coherent Opal Photoactivator is a lab equipment product that provides a controlled source of light for photoactivation experiments. It is designed to emit a specific wavelength of light to trigger photochemical reactions or activate photosensitive materials in a laboratory setting.

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Lab products found in correlation

Visudyne, by Lumenis (3 mentions)

7 protocols using coherent opal photoactivator

1

Photodynamic Lymphatic Ablation in Mice

Cited 2 times
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Mice were anesthetized via i.p. ketamine (100 mg/mL)/xylazine (10 mg/mL). Visudyne (Valeant Ophtalmics) was reconstituted following manufacturer instructions and 5 μL was injected i.c.m. After 15 minutes, Visudyne was photoconverted with a nonthermal 689-nm wavelength laser (Coherent Opal Photoactivator, Lumenis) on 5 different spots through the intact skull (1 on the cisterna magna, 2 on the transverse sinuses, 1 on the superior sagittal sinus, and 1 at the confluence of sinuses). Each spot was irradiated with a light dose of 50 J/cm2 at an intensity of 600 mW/cm2 for 83 s. Mice were sutured and allowed to recover on a heating pad followed by postsurgical analgesic administration. Mice were utilized for experiments seven days after lymphatic ablation. Functional parameters following Visudyne treatment and lack of potential off target effects have been thoroughly characterized using an identical paradigm (Da Mesquita et al., 2018 (link); Louveau et al., 2018 (link)).
Rustenhoven J., Drieu A., Mamuladze T., de Lima K.A., Dykstra T., Wall M., Papadopoulos Z., Kanamori M., Salvador A.F., Baker W., Lemieux M., Da Mesquita S., Cugurra A., Fitzpatrick J., Sviben S., Kossina R., Bayguinov P., Townsend R.R., Zhang Q., Erdmann-Gilmore P., Smirnov I., Lopes M.B., Herz J, & Kipnis J. (2021). Functional characterization of the dural sinuses as a neuroimmune interface. Cell, 184(4), 1000-1016.e27.
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2

Selective Meningeal Lymphatic Ablation

Cited 2 times
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Visudyne treatment was adapted from published protocols17 (link),18 (link),43 (link). Selective ablation of the meningeal lymphatic vessels was achieved by i.c.m. injection and transcranial photoconversion of Visudyne (verteporfin for injection, Valeant Ophthalmics). Visudyne was reconstituted following the manufacturer’s instructions and 5 μl was injected i.c.m. following the procedure described above in the intra-cisterna magna injections methods section. After 15 min, a midline incision was created in the skin to expose the skull and visudyne was photoconverted by pointing a 689-nm-wavelength non-thermal red light (Coherent Opal Photoactivator, Lumenis) to five different locations above the intact skull (1 at the injection site, 1 at the SSS, 1 at the confluence of the sinuses and 2 at the TSs). This experimental group is labeled as Ablated or Visudyne + laser. Each location was irradiated with a light dose of 50 J/cm2 at an intensity of 600 mW/cm2 for a total of 83 s. Controls were injected with the same volume of Visudyne (without the photoconversion step; labeled as Visudyne or Not Ablated) or sterile saline plus laser treatment (labeled as Vehicle + laser). The scalp skin was then sutured, after which the mice were subcutaneously injected with ketoprofen (1 mg/kg) and allowed to recover on a heating pad until fully awake.
Bolte A.C., Dutta A.B., Hurt M.E., Smirnov I., Kovacs M.A., McKee C.A., Ennerfelt H.E., Shapiro D., Nguyen B.H., Frost E.L., Lammert C.R., Kipnis J, & Lukens J.R. (2020). Meningeal lymphatic dysfunction exacerbates traumatic brain injury pathogenesis. Nature Communications, 11, 4524.
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3

Meningeal and Nasal Lymphatic Ablation

Cited 1 time
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Adapted from previous publications28 (link),51 (link),52 (link). Mice were anesthetized with ketamine/xylazine (i.p.). Visudyne® (Valeant Ophtalmics) was reconstituted following manufacturer instructions and 5µl was injected either i.c.m into the cisterna magna (for meningeal lymphatic ablation) or 2µl in each nostril (for nasal lymphatics ablation). After 15 min, Visudyne was photo-converted with a non-thermal 689nm wavelength laser light (Coherent Opal Photoactivator, Lumenis) on 5 different spots through the intact skull (1 on the cisterna magna, 2 on the transverse sinuses, 1 on the superior sagittal sinus and 1 at the junction of all sinuses). For nasal lymphatic ablation, the laser was applied through the soft palate of the mouse’s mouth. Each spot was irradiated with a light dose of 50J/cm2 at an intensity of 600mW/cm2 for 83s. Mice were then sutured and allowed to recover on a heating pad until responsive. Post-surgery, mice were given analgesic subcutaneously.
Louveau A., Herz J., Alme M.N., Salvador A.F., Dong M.Q., Viar K.E., Herod G., Knopp J., Setliff J., Lupi A.L., Mesquita S.D., Frost E.L., Gaultier A., Harris T.H., Cao R., Hu S., Lukens J.R., Smirnov I., Overall C.C., Oliver G, & Kipnis J. (2018). CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature. Nature neuroscience, 21(10), 1380-1391.
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4

Ablation of Meningeal Lymphatic Vessels

Cited 2 times
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Selective ablation of the meningeal lymphatic vessels was achieved by injection of Visudyne (Vis.) and consecutive transcranial photoconversion (photo.) steps following previously described methodology and regimens5 (link),12 (link). Visudyne was reconstituted following manufacturer’s instructions, aliquoted and kept at −20°C until further used. Immediately upon being thawed, Visudyne was injected into the CSF (i.c.m.) and 15 min later, an incision was performed in the skin to expose the skull bone and photoconvert the drug by pointing a 689-nm-wavelength nonthermal red light (Coherent Opal Photoactivator, Lumenis) on 5 different spots above the intact skull (close to the injection site, above the superior sagittal sinus close to the rostral rhinal vein, above the confluence of sinuses and above each transverse sinus). Each spot was irradiated with a light dose of 50 J/cm2 at an intensity of 600 mW/cm2 for a total of 83 s. Controls were injected with the same volume of Visudyne only, without the photoconversion step. The scalp skin was then sutured, the mice were allowed to recover on a heating pad until fully awake and subcutaneously injected with ketoprofen (2 mg/kg).
Da Mesquita S., Papadopoulos Z., Dykstra T., Brase L., Farias F.G., Wall M., Jiang H., Kodira C.D., de Lima K.A., Herz J., Louveau A., Goldman D.H., Salvador A.F., Onengut-Gumuscu S., Farber E., Dabhi N., Kennedy T., Milam M.G., Baker W., Smirnov I., Rich S.S., Benitez B.A., Karch C.M., Perrin R.J., Farlow M., Chhatwal J.P., Holtzman D.M., Cruchaga C., Harari O, & Kipnis J. (2021). Meningeal lymphatics modulate microglial activation and immunotherapy in Alzheimer’s disease. Nature, 593(7858), 255-260.
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5

Selective Meningeal Lymphatic Ablation

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Selective ablation of the meningeal lymphatic vessels was achieved by i.c.m. injection and transcranial photoconversion of Visudyne® (verteporfin for injection, Valeant Ophtalmics). Visudyne was reconstituted following manufacturer instructions and 5 μL was injected i.c.m. following the previously described procedure. After 15 min, an incision was performed in the skin to expose the skull bone and Visudyne was photoconverted by pointing a 689-nm wavelength non-thermal red light (Coherent Opal Photoactivator, Lumenis) on 5 different spots above the intact skull (1 on the injection site, 1 on the superior sagittal sinus, 1 at the junction of all sinuses and 2 on the transverse sinuses). Each spot was irradiated with a light dose of 50 J/cm2 at an intensity of 600 mW/cm2 for a total of 83 s. Controls were injected with the same volume of Visudyne (without the photoconversion step) or sterile saline plus photoconversion (vehicle/photoconversion). The scalp skin was then sutured, the mice were subcutaneously injected with ketoprofen (2 mg/Kg) and allowed to recover on a heating pad until fully awake.
Da Mesquita S., Louveau A., Vaccari A., Smirnov I., Cornelison R.C., Kingsmore K.M., Contarino C., Onengut-Gumuscu S., Farber E., Raper D., Viar K.E., Powell R.D., Baker W., Dabhi N., Bai R., Cao R., Hu S., Rich S.S., Munson J.M., Lopes M.B., Overall C.C., Acton S.T, & Kipnis J. (2018). Functional aspects of meningeal lymphatics in aging and Alzheimer’s disease. Nature, 560(7717), 185-191.
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6

Meningeal and Nasal Lymphatic Ablation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Adapted from previous publications28 (link),51 (link),52 (link). Mice were anesthetized with ketamine/xylazine (i.p.). Visudyne® (Valeant Ophtalmics) was reconstituted following manufacturer instructions and 5µl was injected either i.c.m into the cisterna magna (for meningeal lymphatic ablation) or 2µl in each nostril (for nasal lymphatics ablation). After 15 min, Visudyne was photo-converted with a non-thermal 689nm wavelength laser light (Coherent Opal Photoactivator, Lumenis) on 5 different spots through the intact skull (1 on the cisterna magna, 2 on the transverse sinuses, 1 on the superior sagittal sinus and 1 at the junction of all sinuses). For nasal lymphatic ablation, the laser was applied through the soft palate of the mouse’s mouth. Each spot was irradiated with a light dose of 50J/cm2 at an intensity of 600mW/cm2 for 83s. Mice were then sutured and allowed to recover on a heating pad until responsive. Post-surgery, mice were given analgesic subcutaneously.
Louveau A., Herz J., Alme M.N., Salvador A.F., Dong M.Q., Viar K.E., Herod G., Knopp J., Setliff J., Lupi A.L., Mesquita S.D., Frost E.L., Gaultier A., Harris T.H., Cao R., Hu S., Lukens J.R., Smirnov I., Overall C.C., Oliver G, & Kipnis J. (2018). CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature. Nature neuroscience, 21(10), 1380-1391.
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7

Selective Meningeal Lymphatic Ablation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Selective ablation of the meningeal lymphatic vessels was achieved by i.c.m. injection and transcranial photoconversion of Visudyne® (verteporfin for injection, Valeant Ophtalmics). Visudyne was reconstituted following manufacturer instructions and 5 μL was injected i.c.m. following the previously described procedure. After 15 min, an incision was performed in the skin to expose the skull bone and Visudyne was photoconverted by pointing a 689-nm wavelength non-thermal red light (Coherent Opal Photoactivator, Lumenis) on 5 different spots above the intact skull (1 on the injection site, 1 on the superior sagittal sinus, 1 at the junction of all sinuses and 2 on the transverse sinuses). Each spot was irradiated with a light dose of 50 J/cm2 at an intensity of 600 mW/cm2 for a total of 83 s. Controls were injected with the same volume of Visudyne (without the photoconversion step) or sterile saline plus photoconversion (vehicle/photoconversion). The scalp skin was then sutured, the mice were subcutaneously injected with ketoprofen (2 mg/Kg) and allowed to recover on a heating pad until fully awake.
Da Mesquita S., Louveau A., Vaccari A., Smirnov I., Cornelison R.C., Kingsmore K.M., Contarino C., Onengut-Gumuscu S., Farber E., Raper D., Viar K.E., Powell R.D., Baker W., Dabhi N., Bai R., Cao R., Hu S., Rich S.S., Munson J.M., Lopes M.B., Overall C.C., Acton S.T, & Kipnis J. (2018). Functional aspects of meningeal lymphatics in aging and Alzheimer’s disease. Nature, 560(7717), 185-191.
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