Sc 72956 sh

Small interfering RNAs (siRNAs) directed against human COL11A1 (sc-72956-SH), and a non-targeting negative control target (sc-108060) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). COL11A1 cDNA plasmid (BC117697, GE Healthcare) was cloned into a pCMV6-AC-GFP vector (PS100010, OriGene), followed by verification via sequencing. SUMO-3 (HG12782-UT) cDNA plasmid was purchased from Sino Biological (Beijing, China). miR-509-3p mimics (MC12984), mimics negative control (4464058), miR-509-3p inhibitor (MH12984), and inhibitor negative control (4,464,076) were purchased from Ambion (Foster City, CA, USA). A2780CP70 or OVCAR-8 cells were transfected with miR-509-3p mimics and SUMO-3 in combination using Lipofectamine 3000 (Thermo Fisher Scientific).

The siRNAs directed against human COL11A1 (COL11A1 shRNA, sc-72956-SH, pools of three target-specific 19–25 nt siRNAs) and the non-targeting negative control shRNA plasmid (sc-108060-SH) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). To establish stable clones, the COL11A1 knockdown plasmids were transfected into A2780CP70 cells using the Lipofectamine transfection reagent (Invitrogen). Twenty-four hours after transfection, stable transfectants were selected in G418 (Sigma) at a concentration of 800 μg/mL. Thereafter, the selection medium was replaced every 3 days. After 2 weeks of selection in G418, clones of resistant cells were isolated and allowed to proliferate in medium containing G418 at 800 μg/mL.

Small interfering RNAs (siRNAs) directed against human COL11A1 (sc-72956-SH), and a non-targeting negative control target (sc-108060) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). COL11A1 cDNA plasmid (BC117697, GE Healthcare) was cloned into a pCMV6-AC-GFP vector (PS100010, OriGene), followed by verification via sequencing. SUMO-3 (HG12782-UT) cDNA plasmid was purchased from Sino Biological (Beijing, China). miR-509–3p mimics (MC12984), mimics negative control (4464058), miR-509–3p inhibitor (MH12984), and inhibitor negative control (4464076) were purchased from Ambion (Foster City, CA, USA). A2780CP70 or OVCAR-8 cells were transfected with miR-509–3p mimics and SUMO-3 in combination using Lipofectamine 3000 (Thermo Fisher Scientific).