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Calcein am

Manufactured by Calibre Scientific
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Sourced in Germany

Calcein-AM is a cell-permeant fluorescent dye used to detect viable cells. It is non-fluorescent until the acetomethoxy group is cleaved by intracellular esterases, resulting in a fluorescent compound that is retained within live cells.

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Lab products found in correlation

Tariquidar, by MedChemExpress (3 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (3 mentions) Ku 60019, by LC Laboratories (1 mentions) Annexin 5 fitc, by BD (1 mentions) Complete easypacks protease inhibitors cocktail, by Roche (1 mentions)

3 protocols using calcein am

1

Cytotoxicity Assay with Cell Viability Markers

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MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany), annexin-V-FITC from BD Bioscience (San Jose, CA, USA); tariquidar (p-glycoprotein inhibitor) from MedChemExpress (Monmouth Junction, NJ, USA); calcein-AM from BIOZOL (Eching, Germany); VE-822 (ATR inhibitor), LY2603618 (Chk1 inhibitor), KU-60019 (ATM inhibitor), and PV1019 (Chk2 inhibitor) from LC Laboratories (Woburn, MA, USA); Anisomycin—from NeoCorp (Weilheim, Germany). Docetaxel, and doxorubicin—from a Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany). Primary and secondary antibodies used are listed in Supplementary Table S1.
Dyshlovoy S.A., Mansour W.Y., Ramm N.A., Hauschild J., Zhidkov M.E., Kriegs M., Zielinski A., Hoffer K., Busenbender T., Glumakova K.A., Spirin P.V., Prassolov V.S., Tilki D., Graefen M., Bokemeyer C, & von Amsberg G. (2024). Synthesis and new DNA targeting activity of 6- and 7-tert-butylfascaplysins. Scientific Reports, 14, 11788.
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2

Cytotoxicity Evaluation Methodology

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Calcein-AM was purchased from BIOZOL (Eching, Germany); tariquidar - from MedChemExpress (Monmouth Junction, NJ, USA); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) - from Sigma (Taufkirchen, Germany); cOmplete™ EASYpacks protease inhibitors cocktail and PhosphoSTOP™ EASYpacks - from Roche (Mannheim, Germany); docetaxel and cisplatin - from the Pharmaceutical Department of the University Hospital Hamburg-Eppendorf (Hamburg, Germany). Primary and secondary antibodies used are listed in Supplementary Table S1.
Dyshlovoy S.A., Shubina L.K., Makarieva T.N., Hauschild J., Strewinsky N., Guzii A.G., Menshov A.S., Popov R.S., Grebnev B.B., Busenbender T., Oh-Hohenhorst S.J., Maurer T., Tilki D., Graefen M., Bokemeyer C., Stonik V.A, & von Amsberg G. (2022). New diterpenes from the marine sponge Spongionella sp. overcome drug resistance in prostate cancer by inhibition of P-glycoprotein. Scientific Reports, 12, 13570.
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3

Cytotoxicity Assay with Calcein-AM

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Calcein-AM was purchased from BIOZOL (Eching, Germany), Tariquidar was purchased from MedChemExpress (Monmouth Junction, NJ, USA), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany) and docetaxel was purchased from a Pharmacy of the University Medical Center Hamburg-Eppendorf (Hamburg, Germany).
Leshchenko E.V., Antonov A.S., Borkunov G.V., Hauschild J., Zhuravleva O.I., Khudyakova Y.V., Menshov A.S., Popov R.S., Kim N.Y., Graefen M., Bokemeyer C., von Amsberg G., Yurchenko A.N, & Dyshlovoy S.A. (2023). New Bioactive β-Resorcylic Acid Derivatives from the Alga-Derived Fungus Penicillium antarcticum KMM 4685. Marine Drugs, 21(3), 178.
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