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Human intesticult organoid differentiation media

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Human IntestiCult organoid differentiation media is a specialized culture medium designed for the differentiation and maintenance of human intestinal organoids. The media supports the growth and development of these three-dimensional tissue models derived from human intestinal stem cells.

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Lab products found in correlation

Human recombinant ifn gamma, by BioLegend (2 mentions)

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Intesticult (16 citations) IntestiCult media (4 citations) Human Intesticult (3 citations)

2 protocols using human intesticult organoid differentiation media

1

Intestinal Organoid Differentiation

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COLON MAP organoids were cultured in appropriately supplemented Human IntestiCult organoid growth media (OGM) for 3 days. They either remained in OGM for control or switched to supplemented Human IntestiCult organoid differentiation media (ODM) (STEMCELL Technologies) for 3 more days. For IFN gamma treatment, human recombinant IFN-gamma (Biolegend) was added to each media condition at 100 ng/mL for 24 hours prior to harvesting.
Chen B., Scurrah C.R., McKinley E.T., Simmons A.J., Ramirez-Solano M.A., Zhu X., Markham N.O., Heiser C.N., Vega P.N., Rolong A., Kim H., Sheng Q., Drewes J.L., Zhou Y., Southard-Smith A.N., Xu Y., Ro J., Jones A.L., Revetta F., Berry L.D., Niitsu H., Islam M., Pelka K., Hofree M., Chen J.H., Sarkizova S., Ng K., Giannakis M., Boland G.M., Aguirre A.J., Anderson A.C., Rozenblatt-Rosen O., Regev A., Hacohen N., Kawasaki K., Sato T., Goettel J.A., Grady W.M., Zheng W., Washington M.K., Cai Q., Sears C.L., Goldenring J.R., Franklin J.L., Su T., Huh W.J., Vandekar S., Roland J.T., Liu Q., Coffey R.J., Shrubsole M.J, & Lau K.S. (2021). Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell, 184(26), 6262-6280.e26.
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2

Intestinal Organoid Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
COLON MAP organoids were cultured in appropriately supplemented Human IntestiCult organoid growth media (OGM) for 3 days. They either remained in OGM for control or switched to supplemented Human IntestiCult organoid differentiation media (ODM) (STEMCELL Technologies) for 3 more days. For IFN gamma treatment, human recombinant IFN-gamma (Biolegend) was added to each media condition at 100 ng/mL for 24 hours prior to harvesting.
Chen B., Scurrah C.R., McKinley E.T., Simmons A.J., Ramirez-Solano M.A., Zhu X., Markham N.O., Heiser C.N., Vega P.N., Rolong A., Kim H., Sheng Q., Drewes J.L., Zhou Y., Southard-Smith A.N., Xu Y., Ro J., Jones A.L., Revetta F., Berry L.D., Niitsu H., Islam M., Pelka K., Hofree M., Chen J.H., Sarkizova S., Ng K., Giannakis M., Boland G.M., Aguirre A.J., Anderson A.C., Rozenblatt-Rosen O., Regev A., Hacohen N., Kawasaki K., Sato T., Goettel J.A., Grady W.M., Zheng W., Washington M.K., Cai Q., Sears C.L., Goldenring J.R., Franklin J.L., Su T., Huh W.J., Vandekar S., Roland J.T., Liu Q., Coffey R.J., Shrubsole M.J, & Lau K.S. (2021). Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell, 184(26), 6262-6280.e26.
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