Triglycerides and total cholesterol concentrations were determined by enzymatic colorimetric methods using available commercial kits (Wiener lab Group, Argentina). The high-density lipoprotein cholesterol (HDLc) was determined after precipitation of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDLc) with polyanions (dextran sulphate and magnesium chloride). LDL-cholesterol concentration was determined by a two-step homogeneous assay without precipitation. Free fatty acids were determined using available commercial kits (ab65341, Abcam, USA).
Circulating tumor necrosis factor-α (TNF-α) (RAB0479), interleukin-1β (IL-1β) (RAB0277), insulin (RAB0904) (all from SIGMA Aldrich, St. Louis, MO, USA), leptin (ab100773), and adiponectin (ab108784) (Abcam, USA) were performed using commercially available kits according to the manufacturer′s instructions.
Blood glucose concentrations were measured using a glucose meter (Roche Diagnostics GmbH, Mannheim, Germany). The HOMA-IR (Homeostasis model assessment of insulin resistance) index was calculated as [fasting glucose (mg/dl) × fasting insulin (ng/ml)/405] to assess insulin resistance [38 (link)].
Circulating tumor necrosis factor-α (TNF-α) (RAB0479), interleukin-1β (IL-1β) (RAB0277), insulin (RAB0904) (all from SIGMA Aldrich, St. Louis, MO, USA), leptin (ab100773), and adiponectin (ab108784) (Abcam, USA) were performed using commercially available kits according to the manufacturer′s instructions.
Blood glucose concentrations were measured using a glucose meter (Roche Diagnostics GmbH, Mannheim, Germany). The HOMA-IR (Homeostasis model assessment of insulin resistance) index was calculated as [fasting glucose (mg/dl) × fasting insulin (ng/ml)/405] to assess insulin resistance [38 (link)].