Renaissance western blot chemoluminescence reagent

Manufactured by PerkinElmer

Sourced in United Kingdom

The Renaissance Western Blot Chemoluminescence Reagent is a laboratory equipment product used for the detection and visualization of proteins in Western blot analysis. It functions by emitting light when a specific enzyme-labeled secondary antibody binds to the target protein, allowing for the identification and quantification of the protein of interest.

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Lab products found in correlation

Biotin streptavidin horseradish peroxidase system, by GE Healthcare (4 mentions) Ps6 ser235 236, by Cell Signaling Technology (1 mentions) Anti gapdh ab8245, by Abcam (1 mentions) Ab15348, by Abcam (1 mentions) Ab109131, by Abcam (1 mentions) Ab8245, by Abcam (1 mentions) Chemostar imager, by Intas (1 mentions) Anti bcl 2, by BD (1 mentions)

4 protocols using renaissance western blot chemoluminescence reagent

Western Blot Analysis of Cell Signaling

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Samples were prepared as described previously^{40 (link)}. Anti AKT (#4691), pAKT (Ser473) (#4060), BAD (#9239), pBAD (Ser136) (#4366), BCL2 (#4223), BCL2A1 (#14093), BCL-W (#2724), BCLXL (#2764), pGSK3B (Ser9) (#9323), MCL1 (#39224), pMCL1 (Ser159/Thr163) (#4579), S6 (#2317) and pS6 (Ser235/236) (#2211) antibodies were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) antibody was from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin–horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

Quentmeier H., Geffers R., Hauer V., Nagel S., Pommerenke C., Uphoff C.C., Zaborski M, & Drexler H.G. (2022). Inhibition of MCL1 induces apoptosis in anaplastic large cell lymphoma and in primary effusion lymphoma. Scientific Reports, 12, 1085.

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Western Blot Protein Detection

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Samples were prepared as described previously^{75 (link)}. Anti BLIMP1 (#9115), CCND2 (#3741S), S100A4 (#13018S), S100A6 (#13162S) Abs were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) and anti S100A2 (ab 109494) Abs were from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A., Nagel S., Steube K., Uphoff C.C, & Drexler H.G. (2019). The LL-100 panel: 100 cell lines for blood cancer studies. Scientific Reports, 9, 8218.

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Western Blot for ACE2, TMPRSS2, and GAPDH

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Antibodies against ACE2 (ab15348), TMPRSS2 (ab109131), and GAPDH (ab8245) were obtained from Abcam (Cambridge, UK). Western blot samples were prepared as described previously [66 (link)]. Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer; Waltham, MA, USA). Documentation was performed using the digital system ChemoStar Imager (INTAS, Göttingen, Germany).

Pommerenke C., Rand U., Uphoff C.C., Nagel S., Zaborski M., Hauer V., Kaufmann M., Meyer C., Denkmann S.A., Riese P., Eschke K., Kim Y., Safranko Z.M., Kurolt I.C., Markotic A., Cicin-Sain L, & Steenpass L. (2021). Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies. PLoS ONE, 16(8), e0255622.

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Western Blot Analysis of Apoptosis Markers

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Samples were prepared as described previously [26 (link)]. Anti BCL2 (1:500 dilution) and anti MCL1 Abs (1:250) were purchased from Becton Dickinson. Abs against the phosphorylated and unphosphorylated forms of p38 and MAP2K3 (1:10,000 each) and against BCL6 (1:1,000), BCLXL (1:1,000) and MYC (1:2,000) were obtained from Cell Signaling Technology (Leiden, The Netherlands), the GAPDH mouse monoclonal Ab (mAb) (1:5,000) was from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

Quentmeier H., Drexler H.G., Hauer V., MacLeod R.A., Pommerenke C., Uphoff C.C., Zaborski M., Berglund M., Enblad G, & Amini R.M. (2016). Diffuse Large B Cell Lymphoma Cell Line U-2946: Model for MCL1 Inhibitor Testing. PLoS ONE, 11(12), e0167599.

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