K562 cells (ATCC® CCL-243™) were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM) (Gibco) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco) and 1% penicillin-streptomycin (Gibco). HepG2 cells (ATCC® HB-8065™) were cultured in low glucose (1 g/l) DMEM (Gibco), 1% Glutamax (Gibco), 1% non-essential amino acids (NEAA) (Gibco), 10% FBS (Gibco) and 1% penicillin–streptomycin (Gibco). H1 ES cells was a gift from John Chua's lab. The cells were harvested, and the DNA was extracted using DNeasy blood and tissue kit (QIAGEN) for the low-input assays. DNA concentrations were quantified using Qubit dsDNA HS Assay Kit (Thermo Scientific) in Qubit 3.0 (Invitrogen). The cells were stained with CellTrace Calcein Red-Orange AM (Life Technologies), diluted in Phosphate Buffered Saline (PBS) (Gibco) to 1 cell/μl concentration and isolated in 0.2 ml PCR tubes for the single-cell assay. The tubes were observed under the microscope to confirm the presence of a single fluorescently stained cell.
Hb 8065
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HB-8065 is a laboratory centrifuge designed for general-purpose applications. It features a high-capacity rotor that can accommodate a variety of sample sizes and tube types. The centrifuge operates at a maximum speed of 8,000 rpm and can generate a maximum relative centrifugal force (RCF) of 12,100 x g. The unit is equipped with digital speed and time controls for precise operation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Qubit dsdna hs assay kit, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Celltrace calcein red orange am, by Thermo Fisher Scientific (1 mentions)
Ccl 243, by Thermo Fisher Scientific (1 mentions)
Qubit 3, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Dneasy blood and tissue kit, by Qiagen (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Rmpi 1640, by Thermo Fisher Scientific (1 mentions)
Crl 4031, by American Type Culture Collection (1 mentions)
Tryple express, by Thermo Fisher Scientific (1 mentions)
5 protocols using hb 8065
1
Single-Cell DNA Extraction and Staining
2
Culturing Human Cell Lines
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HEK293T and HepG2 cells were purchased from the American Type Culture Collection (ATCC) (CRL11268 for HEK293T and HB8065 for HepG2) and were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 11995) supplemented with 10% (v/v) fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco, 10378) and grown at 37°C with 5% CO2. K562 cells were purchased from ATCC (CCL243) and were cultured in RPMI 1640 (Gibco, 11875) supplemented with 10% (v/v) fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco) and grown at 37°C with 5% CO2. All cell lines were routinely checked to be free of mycoplasma.
3
Antibody Blocking Assay in Human Cell Lines
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Human hepatocellular carcinoma cells HepG2 (ATCC# HB-8065), human embryonic hepatic cells HL-7702 (L02)10 (link), human cervical carcinoma cell HeLa(ATCC#CCL-2), human leukemic monocyte lymphoma cell U937(ATCC# CRL-1593.2), and HUVECs (ATCC# CRL-1730) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, USA) containing 10% fetal bovine serum (FBS; Hyclone, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin. The cells were maintained in cell culture flask (Thermo Fisher Scitific, USA) in a humidified chamber at 37°C with 5% CO2.
For antidody blocking procedure, cells (80,000/well) were seeded in a 24-well plate 24h before the antibodies were added to culture. 50 μg/ml mouse monoclonal anti-VASN antibody, nonspecific mouse IgG, or no treatment (mock group) were added to the cell culture and further incubated at 37°C. 24 hours later, cells were washed with 1 mL of PBS and harvested for further experiments.
For antidody blocking procedure, cells (80,000/well) were seeded in a 24-well plate 24h before the antibodies were added to culture. 50 μg/ml mouse monoclonal anti-VASN antibody, nonspecific mouse IgG, or no treatment (mock group) were added to the cell culture and further incubated at 37°C. 24 hours later, cells were washed with 1 mL of PBS and harvested for further experiments.
4
Culturing and Maintenance of Diverse Liver Cell Lines
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Human normal cells LO2 was obtained from laboratory preservation and cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco, USA) added with 100 units/mL of streptomycin and 100 units/mL of penicillin. Purchased from American Type Culture Collection (ATCC HB-8065, Rockville, MD, USA), HepG2 was cultured in DMEM, containing 10% fetal bovine serum (FBS, Gibco, Life Technologies, Melbourne, Australia). Human hepatoma cell line (Huh7) and Human HCC cell line SMMC-7721 were preserved in laboratory. Huh7 was cultured in DMEM and supplemented with 10% FBS, 10 mM HEPES, 100 units/mL streptomycin and 100 units/mL of penicillin. SMMC-7721 was grown in RMPI 1640(1640, Gibco, USA), which contains 10% FBS, 100 units/mL of streptomycin and 100 units/mL of penicillin. All cell lines were cultured in a 5% CO2-humidified incubator at 37 °C.
5
Culture of Diverse Renal Cell Lines
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